Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HFR56_RS03875 Genome accession   NZ_CP053055
Coordinates   800748..801728 (+) Length   326 a.a.
NCBI ID   WP_001619985.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Saintpaul strain CVM 22543     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795748..806728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFR56_RS03845 (HFR56_03845) - 796325..797332 (+) 1008 WP_052944280.1 DUF1202 family protein -
  HFR56_RS03850 (HFR56_03850) hemW 797424..798560 (-) 1137 WP_023214414.1 radical SAM family heme chaperone HemW -
  HFR56_RS03855 (HFR56_03855) - 798553..799146 (-) 594 WP_052944281.1 XTP/dITP diphosphatase -
  HFR56_RS03860 (HFR56_03860) yggU 799154..799444 (-) 291 WP_001277204.1 DUF167 family protein YggU -
  HFR56_RS03865 (HFR56_03865) - 799441..800007 (-) 567 WP_001094848.1 YggT family protein -
  HFR56_RS03870 (HFR56_03870) - 800026..800730 (-) 705 WP_052944282.1 YggS family pyridoxal phosphate-dependent enzyme -
  HFR56_RS03875 (HFR56_03875) pilT 800748..801728 (+) 981 WP_001619985.1 type IV pilus twitching motility protein PilT Machinery gene
  HFR56_RS03880 (HFR56_03880) - 801861..802547 (+) 687 WP_052944283.1 IclR family transcriptional regulator domain-containing protein -
  HFR56_RS03885 (HFR56_03885) ruvX 802594..803010 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  HFR56_RS03890 (HFR56_03890) - 803010..803573 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  HFR56_RS03895 (HFR56_03895) gshB 803789..804736 (-) 948 WP_000593243.1 glutathione synthase -
  HFR56_RS03900 (HFR56_03900) rsmE 804756..805487 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HFR56_RS03905 (HFR56_03905) endA 805564..806271 (-) 708 WP_000286133.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36149.35 Da        Isoelectric Point: 7.5116

>NTDB_id=442511 HFR56_RS03875 WP_001619985.1 800748..801728(+) (pilT) [Salmonella enterica subsp. enterica serovar Saintpaul strain CVM 22543]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRAHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=442511 HFR56_RS03875 WP_001619985.1 800748..801728(+) (pilT) [Salmonella enterica subsp. enterica serovar Saintpaul strain CVM 22543]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAGCGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGTGCTACTGGCTGCGGAAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

46.395

97.853

0.454
  pilT Pseudomonas stutzeri DSM 10701

46.203

96.933

0.448
  pilT Pseudomonas aeruginosa PAK

46.203

96.933

0.448
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.858

100

0.374