Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HJ586_RS21255 Genome accession   NZ_CP053014
Coordinates   4745298..4746320 (+) Length   340 a.a.
NCBI ID   WP_236305833.1    Uniprot ID   -
Organism   Dickeya zeae strain MS_2018     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4740298..4751320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HJ586_RS21225 (HJ586_21240) hemW 4741027..4742166 (-) 1140 WP_236305831.1 radical SAM family heme chaperone HemW -
  HJ586_RS21230 (HJ586_21245) - 4742159..4742752 (-) 594 WP_236305832.1 XTP/dITP diphosphatase -
  HJ586_RS21235 (HJ586_21250) yggU 4742800..4743090 (-) 291 WP_012886029.1 DUF167 family protein YggU -
  HJ586_RS21240 (HJ586_21255) - 4743087..4743641 (-) 555 WP_023640669.1 YggT family protein -
  HJ586_RS21245 (HJ586_21260) proC 4743685..4744506 (-) 822 WP_102802290.1 pyrroline-5-carboxylate reductase -
  HJ586_RS21250 (HJ586_21265) - 4744539..4745276 (-) 738 WP_329610151.1 YggS family pyridoxal phosphate-dependent enzyme -
  HJ586_RS21255 (HJ586_21270) pilT 4745298..4746320 (+) 1023 WP_236305833.1 type IV pilus twitching motility protein PilT Machinery gene
  HJ586_RS21260 (HJ586_21275) - 4746463..4748535 (-) 2073 WP_236305834.1 EAL domain-containing protein -
  HJ586_RS21265 (HJ586_21280) codA 4748813..4750054 (-) 1242 WP_236305839.1 cytosine deaminase -
  HJ586_RS21270 (HJ586_21285) - 4750057..4751028 (-) 972 WP_236305841.1 PucR family transcriptional regulator -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37150.25 Da        Isoelectric Point: 6.1191

>NTDB_id=442126 HJ586_RS21255 WP_236305833.1 4745298..4746320(+) (pilT) [Dickeya zeae strain MS_2018]
MDVSEWVNASVKQNVSDLHLCSGHPPVWRIDGQLVTDVTQLPLQHTDLEQWSQSWLDPECEQQLRRCGQTDCALTLAGGQ
RLRANLFMQRHGLSAALRLIPHGQPDLQTLRAPAVVAALAERTDGLILVTGATGSGKSTTLAAMIAALNQRSARHIITLE
DPIEFLHTSQRSLIQQREIGRHSEGFSSALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDVFPAEEKAFVRTQLAASLQAVVAQKLLPGAQGGRVALFEVLLATPAVSNLIREGKTHQLPSLLQTGSQVGMQTFEQS
YQQRCQEGLLADDCADDSWL

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=442126 HJ586_RS21255 WP_236305833.1 4745298..4746320(+) (pilT) [Dickeya zeae strain MS_2018]
ATGGATGTGAGTGAATGGGTGAACGCTAGTGTAAAACAGAATGTATCCGATCTGCACCTGTGCAGCGGTCATCCCCCGGT
GTGGCGTATCGACGGGCAACTGGTGACGGATGTTACCCAACTGCCGTTACAGCACACCGACCTGGAACAGTGGAGCCAGA
GCTGGCTGGACCCGGAGTGTGAGCAGCAGTTGCGCCGGTGCGGGCAGACCGACTGTGCGTTGACGCTGGCGGGCGGGCAG
CGGTTGCGGGCCAATCTGTTTATGCAGCGCCACGGTTTATCGGCAGCGCTACGCCTGATCCCACACGGCCAGCCGGATTT
GCAGACGCTGCGGGCACCTGCGGTCGTCGCGGCATTGGCTGAGCGTACCGACGGACTGATTCTGGTGACCGGGGCGACCG
GCAGCGGTAAATCGACCACCCTGGCGGCGATGATCGCGGCACTGAACCAGCGTAGTGCCCGCCATATTATTACGCTGGAA
GACCCGATTGAATTCCTGCACACCAGCCAACGTAGCCTGATCCAACAGCGGGAAATCGGTCGGCACAGTGAAGGATTTTC
CAGCGCTTTGCGGGCCGCATTGCGTGAAGACCCGGATGTGCTGTTACTGGGGGAACTGCGCGATAGCGAGACTATTCGGC
TAGCGCTGACGGCGGCGGAGACCGGCCATTTGGTACTGGCGACGTTGCATACCCGACGGGCGGTGCAGGCGGTGGATAGG
TTAGTGGATGTGTTCCCGGCCGAAGAGAAAGCCTTTGTGCGCACACAACTGGCAGCGAGTTTGCAGGCGGTAGTGGCGCA
GAAATTATTGCCGGGTGCGCAGGGGGGGCGTGTGGCGTTATTTGAGGTACTGCTTGCCACACCGGCGGTCAGTAACCTGA
TCCGCGAAGGTAAAACCCACCAGTTGCCGAGCCTGTTGCAAACCGGCAGCCAGGTGGGGATGCAGACCTTTGAGCAGAGC
TACCAACAGCGGTGTCAGGAAGGCCTGTTAGCGGATGATTGCGCAGACGATAGCTGGCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

48.529

100

0.485
  pilT Vibrio cholerae strain A1552

49.394

97.059

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

97.059

0.479
  pilT Neisseria meningitidis 8013

47.788

99.706

0.476
  pilT Neisseria gonorrhoeae MS11

47.493

99.706

0.474
  pilT Pseudomonas aeruginosa PAK

47.463

98.529

0.468
  pilT Legionella pneumophila strain Lp02

45.882

100

0.459
  pilT Legionella pneumophila strain ERS1305867

45.882

100

0.459
  pilT Acinetobacter baylyi ADP1

46.667

97.059

0.453
  pilT Acinetobacter baumannii D1279779

45.97

98.529

0.453
  pilT Acinetobacter nosocomialis M2

45.97

98.529

0.453
  pilT Acinetobacter baumannii strain A118

45.97

98.529

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.828

93.824

0.421
  pilU Pseudomonas stutzeri DSM 10701

37.971

100

0.385
  pilU Vibrio cholerae strain A1552

37.391

100

0.379