Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J417_RS17510 Genome accession   NZ_CP053012
Coordinates   3900950..3901972 (-) Length   340 a.a.
NCBI ID   WP_019843499.1    Uniprot ID   -
Organism   Dickeya zeae MS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3895950..3906972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J417_RS17495 (J417_17515) - 3896290..3897261 (+) 972 WP_019843502.1 PucR family transcriptional regulator -
  J417_RS17500 (J417_17520) codA 3897264..3898505 (+) 1242 WP_026357762.1 cytosine deaminase -
  J417_RS17505 (J417_17525) - 3898783..3900855 (+) 2073 WP_236377648.1 EAL domain-containing protein -
  J417_RS17510 (J417_17530) pilT 3900950..3901972 (-) 1023 WP_019843499.1 type IV pilus twitching motility protein PilT Machinery gene
  J417_RS17515 (J417_17535) - 3901994..3902731 (+) 738 WP_019843498.1 YggS family pyridoxal phosphate-dependent enzyme -
  J417_RS17520 (J417_17540) proC 3902764..3903585 (+) 822 WP_019843497.1 pyrroline-5-carboxylate reductase -
  J417_RS17525 (J417_17545) - 3903629..3904183 (+) 555 WP_019843496.1 YggT family protein -
  J417_RS17530 (J417_17550) yggU 3904180..3904470 (+) 291 WP_026357760.1 DUF167 family protein YggU -
  J417_RS17535 (J417_17555) - 3904518..3905111 (+) 594 WP_019843494.1 XTP/dITP diphosphatase -
  J417_RS17540 (J417_17560) hemW 3905104..3906243 (+) 1140 WP_019843493.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37166.31 Da        Isoelectric Point: 6.1185

>NTDB_id=442076 J417_RS17510 WP_019843499.1 3900950..3901972(-) (pilT) [Dickeya zeae MS1]
MDVSEWVNASVKQNVSDLHLCSGHPPVWRIDGQLVTDVTQLPLQHTDLEQWSQSWLDPECEQQLRRCGQTDCALTLAGGQ
RLRANLFMQRHGLSAALRLIPHGQPDLQTLRAPAVVAALAERTDGLILVTGATGSGKSTTLAAMIAALNQRCARHIITLE
DPIEFLHTSQRSLIQQREIGRHSEGFSSALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDVFPAEEKAFVRTQLAASLQAVVAQKLLPGAQGGRVALFEVLLATPAVSNLIREGKTHQLPSLLQTGSQVGMQTFEQS
YQQRCQEGLLADDCADDSWL

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=442076 J417_RS17510 WP_019843499.1 3900950..3901972(-) (pilT) [Dickeya zeae MS1]
ATGGATGTGAGTGAATGGGTGAACGCTAGTGTAAAACAGAATGTATCCGATCTGCACCTGTGCAGCGGTCATCCCCCGGT
GTGGCGTATCGACGGGCAACTGGTGACGGATGTTACCCAACTGCCGTTACAGCACACCGACCTGGAACAGTGGAGCCAGA
GCTGGCTGGACCCGGAGTGTGAGCAGCAGTTGCGCCGGTGCGGGCAGACCGACTGTGCGTTGACGCTGGCGGGCGGGCAG
CGGTTGCGGGCCAATCTGTTTATGCAGCGCCACGGTTTATCGGCAGCGCTACGCCTGATCCCACACGGCCAGCCGGATTT
GCAGACGCTGCGGGCACCTGCGGTCGTCGCGGCATTGGCTGAGCGTACCGACGGACTGATTCTGGTGACCGGGGCGACCG
GTAGCGGTAAATCGACCACCCTGGCGGCGATGATCGCGGCACTGAACCAGCGTTGTGCCCGCCATATTATTACGTTGGAA
GACCCGATTGAATTCCTGCACACCAGCCAACGTAGCCTGATCCAACAGCGGGAAATCGGTCGGCACAGTGAAGGATTTTC
CAGCGCTTTGCGGGCCGCATTGCGTGAAGATCCGGATGTGCTGTTACTGGGGGAACTGCGCGATAGCGAGACTATTCGGC
TAGCGCTGACGGCGGCGGAGACCGGCCATTTGGTACTGGCGACGTTGCATACCCGACGGGCGGTGCAGGCGGTGGATAGG
TTAGTGGATGTGTTCCCGGCCGAAGAGAAAGCCTTTGTGCGCACACAACTGGCAGCGAGTTTGCAGGCGGTAGTGGCGCA
GAAATTATTGCCGGGTGCGCAGGGTGGGCGTGTGGCGTTATTTGAGGTACTGCTTGCCACACCGGCGGTCAGTAACCTGA
TCCGCGAAGGTAAAACCCACCAGTTGCCGAGCCTGTTGCAAACCGGCAGCCAGGTGGGGATGCAGACCTTTGAGCAGAGC
TACCAACAGCGGTGTCAGGAAGGCCTGTTAGCGGATGACTGCGCAGACGATAGCTGGCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

48.529

100

0.485
  pilT Vibrio cholerae strain A1552

49.394

97.059

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

97.059

0.479
  pilT Neisseria meningitidis 8013

47.788

99.706

0.476
  pilT Neisseria gonorrhoeae MS11

47.493

99.706

0.474
  pilT Pseudomonas aeruginosa PAK

47.463

98.529

0.468
  pilT Legionella pneumophila strain Lp02

45.882

100

0.459
  pilT Legionella pneumophila strain ERS1305867

45.882

100

0.459
  pilT Acinetobacter baylyi ADP1

46.667

97.059

0.453
  pilT Acinetobacter baumannii D1279779

45.97

98.529

0.453
  pilT Acinetobacter nosocomialis M2

45.97

98.529

0.453
  pilT Acinetobacter baumannii strain A118

45.97

98.529

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.828

93.824

0.421
  pilU Pseudomonas stutzeri DSM 10701

37.681

100

0.382
  pilU Vibrio cholerae strain A1552

37.391

100

0.379