Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HFS24_RS03435 Genome accession   NZ_CP051368
Coordinates   725923..726903 (+) Length   326 a.a.
NCBI ID   WP_219827110.1    Uniprot ID   -
Organism   Salmonella enterica subsp. houtenae serovar 43:z4 strain CVM 24399     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 720923..731903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFS24_RS03405 (HFS24_03400) ansB 721457..722503 (+) 1047 WP_219827109.1 L-asparaginase 2 -
  HFS24_RS03410 (HFS24_03405) hemW 722599..723735 (-) 1137 WP_114049534.1 radical SAM family heme chaperone HemW -
  HFS24_RS03415 (HFS24_03410) - 723728..724321 (-) 594 WP_001174765.1 XTP/dITP diphosphatase -
  HFS24_RS03420 (HFS24_03415) yggU 724329..724619 (-) 291 WP_001277202.1 DUF167 family protein YggU -
  HFS24_RS03425 (HFS24_03420) - 724616..725182 (-) 567 WP_001094848.1 YggT family protein -
  HFS24_RS03430 (HFS24_03425) - 725201..725905 (-) 705 WP_000997811.1 YggS family pyridoxal phosphate-dependent enzyme -
  HFS24_RS03435 (HFS24_03430) pilT 725923..726903 (+) 981 WP_219827110.1 type IV pilus twitching motility protein PilT Machinery gene
  HFS24_RS03440 (HFS24_03435) - 727034..727720 (+) 687 WP_000098330.1 IclR family transcriptional regulator -
  HFS24_RS03445 (HFS24_03440) ruvX 727768..728184 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  HFS24_RS03450 (HFS24_03445) - 728184..728747 (-) 564 WP_001053170.1 YqgE/AlgH family protein -
  HFS24_RS03455 (HFS24_03450) gshB 728960..729907 (-) 948 WP_023187481.1 glutathione synthase -
  HFS24_RS03460 (HFS24_03455) rsmE 729927..730658 (-) 732 WP_001222490.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HFS24_RS03465 (HFS24_03460) endA 730734..731441 (-) 708 WP_002954459.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36062.29 Da        Isoelectric Point: 7.7571

>NTDB_id=437760 HFS24_RS03435 WP_219827110.1 725923..726903(+) (pilT) [Salmonella enterica subsp. houtenae serovar 43:z4 strain CVM 24399]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLFNWLNDAQQCQWRTHGQLDFAVSLAGARRLRA
SAFTHQQGTSLALRLLPERCPELAEIQTPPIVPALLASENGLILVTGATGSGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYASQRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEADRQNGRVALFELLINTPATGNLIREGKLHQLTHVIQTGQQQGMMTFAQCAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=437760 HFS24_RS03435 WP_219827110.1 725923..726903(+) (pilT) [Salmonella enterica subsp. houtenae serovar 43:z4 strain CVM 24399]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGGATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGTTTAATTGGCTTAATG
ACGCGCAGCAATGTCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGGCTGGCGCGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTCTTGCCCGAGCGTTGTCCTGAACTGGCGGAAATCCA
GACGCCGCCGATAGTGCCAGCGCTGCTTGCCAGTGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCAGCGGTAAAT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAACCAACATGCCGATAAACATATTCTGACGCTGGAAGATCCTATTGAG
TACCGTTACGCCAGCCAGCGTTGCCTGATACAACAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCAGGTAGCCTGCGCGCCGTACTATCGCAAAAACTGGAGGC
CGACAGGCAGAATGGGCGCGTAGCGTTGTTTGAATTATTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGTA
AACTTCATCAGTTGACCCATGTAATACAAACCGGGCAGCAGCAGGGGATGATGACGTTCGCCCAGTGTGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.446

100

0.371