Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HFS63_RS03925 Genome accession   NZ_CP051358
Coordinates   806527..807507 (+) Length   326 a.a.
NCBI ID   WP_001055652.1    Uniprot ID   A0A5U3JPW7
Organism   Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 28322     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801527..812507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HFS63_RS03895 (HFS63_03900) - 802104..803111 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  HFS63_RS03900 (HFS63_03905) hemW 803203..804339 (-) 1137 WP_001096521.1 radical SAM family heme chaperone HemW -
  HFS63_RS03905 (HFS63_03910) - 804332..804925 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  HFS63_RS03910 (HFS63_03915) yggU 804933..805223 (-) 291 WP_001277204.1 DUF167 family protein YggU -
  HFS63_RS03915 (HFS63_03920) - 805220..805786 (-) 567 WP_001094848.1 YggT family protein -
  HFS63_RS03920 (HFS63_03925) - 805805..806509 (-) 705 WP_000997787.1 YggS family pyridoxal phosphate-dependent enzyme -
  HFS63_RS03925 (HFS63_03930) pilT 806527..807507 (+) 981 WP_001055652.1 type IV pilus twitching motility protein PilT Machinery gene
  HFS63_RS03930 (HFS63_03935) - 807640..808326 (+) 687 WP_000098325.1 IclR family transcriptional regulator -
  HFS63_RS03935 (HFS63_03940) ruvX 808373..808789 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  HFS63_RS03940 (HFS63_03945) - 808789..809352 (-) 564 WP_001053164.1 YqgE/AlgH family protein -
  HFS63_RS03945 (HFS63_03950) gshB 809568..810515 (-) 948 WP_000593248.1 glutathione synthase -
  HFS63_RS03950 (HFS63_03955) rsmE 810535..811266 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HFS63_RS03955 (HFS63_03960) endA 811343..812050 (-) 708 WP_000286126.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36167.41 Da        Isoelectric Point: 7.8163

>NTDB_id=437692 HFS63_RS03925 WP_001055652.1 806527..807507(+) (pilT) [Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 28322]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRKHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=437692 HFS63_RS03925 WP_001055652.1 806527..807507(+) (pilT) [Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 28322]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAAAGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTTGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5U3JPW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

45.455

97.853

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365