Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HGD76_RS23925 Genome accession   NZ_CP051206
Coordinates   5008886..5009986 (+) Length   366 a.a.
NCBI ID   WP_148765085.1    Uniprot ID   -
Organism   Dolichospermum flos-aquae CCAP 1403/13F     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5003886..5014986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGD76_RS23915 (HGD76_23920) grpE 5005305..5005979 (-) 675 WP_148765081.1 nucleotide exchange factor GrpE -
  HGD76_RS23920 (HGD76_23925) pilB/pilB1 5006821..5008821 (+) 2001 WP_168697242.1 GspE/PulE family protein Machinery gene
  HGD76_RS23925 (HGD76_23930) pilT 5008886..5009986 (+) 1101 WP_148765085.1 type IV pilus twitching motility protein PilT Machinery gene
  HGD76_RS23930 (HGD76_23935) - 5009978..5010127 (-) 150 WP_168204467.1 hypothetical protein -
  HGD76_RS23935 (HGD76_23940) - 5010159..5011382 (+) 1224 WP_148765087.1 type II secretion system F family protein -
  HGD76_RS23940 (HGD76_23945) - 5011576..5013468 (+) 1893 WP_168697243.1 GTPase family protein -
  HGD76_RS23945 (HGD76_23950) - 5013710..5014564 (+) 855 WP_168697244.1 bile acid:sodium symporter family protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40161.45 Da        Isoelectric Point: 7.3523

>NTDB_id=436934 HGD76_RS23925 WP_148765085.1 5008886..5009986(+) (pilT) [Dolichospermum flos-aquae CCAP 1403/13F]
MEMMIEDLMEELITRGGSDLHLSAGLPPYFRISGRLTPIGENILTADECQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMTEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPVEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETIALAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPSEKQTQVRVQLSNSLVAVFSQTLVPKKNVKSGEYGRVMAQEIMIITPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAISKTSKPDEVQRLIGATAPAGAKLR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=436934 HGD76_RS23925 WP_148765085.1 5008886..5009986(+) (pilT) [Dolichospermum flos-aquae CCAP 1403/13F]
ATGGAAATGATGATTGAAGACTTGATGGAAGAACTGATTACTAGAGGTGGGTCAGACTTGCACCTATCCGCTGGATTGCC
TCCCTATTTTCGGATTAGTGGACGTCTGACACCTATTGGTGAAAACATATTGACGGCTGATGAATGTCAAAGGCTAATTT
TCAGTATGCTCAATAACACTCAGCGCAAAACTTTAGAGCAAACATGGGAATTAGATTGTTCTTACGGTGTCAAAGGATTA
GCTAGGTTCCGGGTGAATGTCTATAAAGAACGTGGTGCTTATGCTGCTTGTTTACGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTTTGCCAGATATAGTCCGGGAAATGACGGAAAAACCTAGAGGACTAATTCTCGTCACAGGTCCAA
CTGGTTCTGGGAAGACTACTACCCTAGCGGCAATGATTGACTTAATTAACCGCACTAGGGCAGAACATATTTTAACAGTT
GAAGACCCTGTGGAATTTGTTTATGAACCAATCAAAAGCCTAGTTCACCAACGCCAATTAGGAGAAGATACGAAGAGTTT
TGCTAATGCTTTAAAAGCAGCTTTACGAGAAGATCCCGATATTATTCTGGTAGGAGAAATGCGGGACTTGGAAACCATTG
CGTTGGCGATTTCCGCCGCAGAAACAGGTCACTTGGTATTTGGTACACTACATACAAGTTCTGCCGCACAAACCGTTGAC
CGGATTATTGACGTTTTTCCCTCAGAAAAACAAACTCAGGTGCGGGTGCAGTTGTCTAACTCCTTAGTCGCAGTTTTTAG
TCAGACTTTAGTACCTAAGAAAAATGTCAAGTCAGGTGAATATGGGCGAGTAATGGCTCAAGAAATTATGATTATCACTC
CCGCTATTTCTAACTTAATTCGGGAAGGAAAAACAGCACAAATTTACTCAGCTATTCAGACGGGTGGTAAATTGGGAATG
CAAACTCTAGAAAAAGTTTTAGCTGATTTGTACAAAGCTGGAACTATTTCTTTTGAAGCAGCAATATCTAAAACTTCTAA
ACCAGATGAAGTCCAGCGTCTAATTGGTGCAACAGCCCCAGCAGGAGCTAAATTACGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.755

94.809

0.519
  pilT Pseudomonas stutzeri DSM 10701

54.467

94.809

0.516
  pilT Legionella pneumophila strain ERS1305867

54.179

94.809

0.514
  pilT Acinetobacter baylyi ADP1

54.179

94.809

0.514
  pilT Legionella pneumophila strain Lp02

54.179

94.809

0.514
  pilT Acinetobacter baumannii strain A118

53.026

94.809

0.503
  pilT Acinetobacter nosocomialis M2

53.026

94.809

0.503
  pilT Acinetobacter baumannii D1279779

53.026

94.809

0.503
  pilT Neisseria gonorrhoeae MS11

51.327

92.623

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.585

93.443

0.473
  pilT Vibrio cholerae strain A1552

50.585

93.443

0.473
  pilT Neisseria meningitidis 8013

51.032

92.623

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.571

95.628

0.464
  pilU Acinetobacter baylyi ADP1

37.705

100

0.377