Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HGD76_RS23920 Genome accession   NZ_CP051206
Coordinates   5006821..5008821 (+) Length   666 a.a.
NCBI ID   WP_168697242.1    Uniprot ID   A0A6H2C4L9
Organism   Dolichospermum flos-aquae CCAP 1403/13F     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5001821..5013821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGD76_RS23910 (HGD76_23915) - 5002911..5004548 (+) 1638 WP_168697441.1 IS1634 family transposase -
  HGD76_RS23915 (HGD76_23920) grpE 5005305..5005979 (-) 675 WP_148765081.1 nucleotide exchange factor GrpE -
  HGD76_RS23920 (HGD76_23925) pilB/pilB1 5006821..5008821 (+) 2001 WP_168697242.1 GspE/PulE family protein Machinery gene
  HGD76_RS23925 (HGD76_23930) pilT 5008886..5009986 (+) 1101 WP_148765085.1 type IV pilus twitching motility protein PilT Machinery gene
  HGD76_RS23930 (HGD76_23935) - 5009978..5010127 (-) 150 WP_168204467.1 hypothetical protein -
  HGD76_RS23935 (HGD76_23940) - 5010159..5011382 (+) 1224 WP_148765087.1 type II secretion system F family protein -
  HGD76_RS23940 (HGD76_23945) - 5011576..5013468 (+) 1893 WP_168697243.1 GTPase family protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74597.51 Da        Isoelectric Point: 6.1195

>NTDB_id=436933 HGD76_RS23920 WP_168697242.1 5006821..5008821(+) (pilB/pilB1) [Dolichospermum flos-aquae CCAP 1403/13F]
MTYSSPQRRSTALTTRTEFSPFGNKLVTSGYVNNEQMKQAVIASRKSGKPLTEVLESITERKLSPELVTEYKKQHLFELK
ILYGVESLDPEIYEIDTTNVGNLIQTLIPVDICRRHHLIPLSRNDNQSPPYVLVAMVDPDNLDASDDLNQILRSQGLSFQ
RRVITQKDYQDIINKFLNEQAARRKILDRQKNYDVKGDLDNLGTLDIDESSDNDVDLDNAMKGADHAPIIKLVNRILLKA
LTEGVSDIHIEPQEEYLRIRFRKDGVLHEAFDNMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRIFAGRKVDFRVNT
LPSRYGEKVVVRILDNSTTQLGLDKLITDPETLNIVKDMFSKPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFSTVLRAFLRQDPDVLLVGETRDRETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMISSSLIGVLAQRLVRRVCAECRIAYTPTIKELARYGLSVSQEAQVTFYKANTIPPEEIASHKNLCQKCGGVGYK
GRCGVYEVMRITEELQTLINKEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTLTDKGLEAELKAKRKSGL
TCLTCSGELQPEWLDCPYCMTPCFTD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=436933 HGD76_RS23920 WP_168697242.1 5006821..5008821(+) (pilB/pilB1) [Dolichospermum flos-aquae CCAP 1403/13F]
ATGACTTACTCATCACCACAACGGCGTAGCACTGCGCTGACTACAAGAACTGAGTTTTCGCCTTTCGGTAATAAATTAGT
AACGTCTGGCTATGTCAATAATGAACAAATGAAGCAGGCTGTAATTGCCAGCCGCAAGTCCGGTAAACCACTGACAGAAG
TATTAGAATCAATTACTGAGCGAAAATTATCACCTGAGTTGGTCACCGAATACAAAAAACAACATTTATTTGAACTCAAA
ATTTTATACGGCGTTGAGTCTCTTGATCCCGAAATCTACGAAATTGATACAACAAACGTGGGGAATTTAATTCAAACCCT
CATTCCTGTTGATATCTGTCGTCGGCATCACTTAATCCCACTGTCAAGAAATGATAACCAAAGCCCACCTTATGTGTTGG
TGGCTATGGTTGATCCAGATAATTTAGATGCTTCTGATGATCTTAATCAGATTTTGCGTTCCCAAGGCTTGTCTTTTCAA
CGCAGGGTAATTACCCAGAAAGATTATCAGGACATCATTAACAAATTTTTAAATGAACAAGCGGCACGCCGAAAGATATT
AGACCGGCAAAAAAATTATGATGTCAAGGGTGATTTAGACAATCTAGGGACTCTGGACATAGATGAAAGTAGTGATAATG
ATGTTGATTTAGATAATGCTATGAAGGGTGCTGATCATGCACCAATCATTAAACTTGTGAATCGGATTTTGTTGAAGGCT
TTAACTGAGGGTGTTTCGGATATTCACATTGAACCGCAGGAAGAATATTTACGGATTCGTTTCCGTAAGGATGGGGTATT
ACATGAGGCATTTGATAATATGCCGAAAAAAATTATCCCAGCGGTAACAGCCCGATTTAAAATTATTTCTAACCTGGACA
TTGCTGAAAGACGTTTACCTCAAGATGGCCGGATTCGCCGGATATTTGCCGGAAGAAAGGTAGATTTCCGGGTAAATACT
CTACCTAGTCGCTATGGAGAAAAGGTTGTTGTACGGATTTTAGATAACTCTACAACTCAATTAGGTTTGGATAAGTTAAT
TACTGACCCAGAAACCTTAAATATTGTTAAGGATATGTTTAGTAAGCCATTCGGGTTGATTTTAGTGACGGGACCAACTG
GTTCGGGTAAAACTACTTCGTTGTATTCGGCACTATCAGAAAAGAATGATCCGGGGATTAATATCAGTACAGTAGAAGAT
CCAATTGAGTATAGTTTACCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAGGGGTTGGATTTCTCGACGGTCTTGCG
GGCTTTCTTGCGTCAAGATCCAGATGTGTTGCTGGTGGGGGAAACACGGGATCGGGAAACTGCGAAAACGGCGATTGAGG
CAGCATTAACCGGTCACTTGGTATTAACTACTTTACACACTAATGATGCACCAGGAGCGATCGCTCGCTTGGGAGAAATG
GGTATTGAACCTTTCATGATTTCCAGTTCTTTAATTGGTGTTTTAGCACAACGTCTAGTTCGTCGTGTCTGTGCTGAATG
TCGTATAGCATACACTCCGACCATTAAAGAACTAGCTCGCTATGGTCTATCCGTTTCCCAAGAAGCTCAGGTAACTTTCT
ACAAAGCCAATACTATTCCACCAGAAGAAATAGCCAGCCATAAAAATCTTTGTCAAAAATGTGGTGGTGTTGGCTACAAG
GGGCGTTGTGGGGTTTATGAAGTAATGCGGATTACTGAAGAATTACAAACTCTGATCAACAAGGAAGCACCTACAGAAAG
GATTAAAGAAGTAGCTGTTGAAGAGGGGATGAAAACCTTACTAGCTTACAGTTTGGACTTAGTTCGTCAAGGAGCAACTA
CCTTAGAAGAAGTAGAGCGAGTAACACTTACAGATAAAGGTTTAGAGGCTGAATTAAAAGCTAAACGCAAAAGTGGTCTG
ACTTGTCTGACTTGTAGTGGGGAATTACAACCAGAATGGTTAGATTGTCCTTACTGTATGACACCTTGCTTTACAGATTA
G

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-181)

Type II/IV secretion system protein (T2SSE)

(235-502)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H2C4L9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.353

100

0.641