NTDB

Overview

Name	pilA/pilAI	Type	Machinery gene
Locus tag	HER31_RS18945	Genome accession	NZ_CP051180
Coordinates	3870478..3870897 (-)	Length	139 a.a.
NCBI ID	WP_168662620.1	Uniprot ID	A0A6H1UIW2
Organism	Ferrimonas lipolytica strain S7
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3865478..3875897

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HER31_RS17565 (HER31_17565)	coaE	3866068..3866670 (-)	603	WP_168662612.1	dephospho-CoA kinase	-
HER31_RS17570 (HER31_17570)	pilD	3866667..3867548 (-)	882	WP_168662614.1	prepilin peptidase	Machinery gene
HER31_RS17575 (HER31_17575)	pilC	3867582..3868865 (-)	1284	WP_168662616.1	type II secretion system F family protein	Machinery gene
HER31_RS17580 (HER31_17580)	pilB	3868889..3870409 (-)	1521	Protein_3357	type IV-A pilus assembly ATPase PilB	-
HER31_RS18945 (HER31_17585)	pilA/pilAI	3870478..3870897 (-)	420	WP_168662620.1	pilin	Machinery gene
HER31_RS17590 (HER31_17590)	nadC	3871175..3872023 (-)	849	WP_168662622.1	carboxylating nicotinate-nucleotide diphosphorylase	-
HER31_RS17595 (HER31_17595)	-	3872028..3872537 (-)	510	WP_168662624.1	retropepsin-like aspartic protease family protein	-
HER31_RS17600 (HER31_17600)	ampD	3872599..3873165 (+)	567	WP_168662626.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
HER31_RS17605 (HER31_17605)	ampE	3873162..3874001 (+)	840	WP_168662628.1	beta-lactamase regulator AmpE	-
HER31_RS17610 (HER31_17610)	pdhR	3874290..3875048 (+)	759	WP_168662630.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-

Sequence

Protein

Download Length: 139 a.a. Molecular weight: 14680.76 Da Isoelectric Point: 4.5408

                                >NTDB_id=436678 HER31_RS18945 WP_168662620.1 3870478..3870897(-) (pilA/pilAI) [Ferrimonas lipolytica strain S7]

MKRNLQQGFTLIELMIVVAIIGILAAIAIPAYQDYTVKSQVNAALAEVTPYKTAYEIAVNEGETVTTTLGNTISGTYCTI

TVAAPSTTSAAANSLDCKLDSGTVNALVKDKNITLTRDGDGVWDCTSTIPAEFKPKNCS

Nucleotide

Download Length: 420 bp

                                >NTDB_id=436678 HER31_RS18945 WP_168662620.1 3870478..3870897(-) (pilA/pilAI) [Ferrimonas lipolytica strain S7]

ATGAAACGGAACTTACAACAAGGCTTTACCCTGATTGAATTGATGATCGTGGTAGCCATTATCGGTATTTTGGCAGCAAT

CGCGATTCCTGCTTATCAGGATTACACAGTGAAGTCGCAAGTTAATGCTGCGCTTGCTGAAGTCACTCCATATAAAACTG

CATATGAAATTGCAGTAAATGAGGGTGAAACTGTAACCACCACTCTTGGAAACACTATTAGTGGAACCTACTGCACAATT

ACAGTCGCTGCTCCTAGTACAACAAGTGCCGCAGCAAACTCGTTAGATTGTAAGCTAGATTCAGGAACTGTGAACGCTTT

AGTTAAAGATAAAAATATCACTCTAACTAGAGATGGCGATGGTGTTTGGGATTGCACTTCTACAATCCCAGCTGAATTTA

AGCCAAAAAATTGTAGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6H1UIW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	50.345	100	0.525
pilA	Acinetobacter baumannii strain A118	49.645	100	0.504
pilA	Pseudomonas aeruginosa PAK	44.371	100	0.482
pilA/pilAII	Pseudomonas stutzeri DSM 10701	47.826	99.281	0.475
comP	Acinetobacter baylyi ADP1	40.541	100	0.432
pilA	Vibrio cholerae C6706	40.136	100	0.424
pilA	Vibrio cholerae strain A1552	40.136	100	0.424
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	40.136	100	0.424
pilA2	Legionella pneumophila strain ERS1305867	41.176	97.842	0.403
pilA2	Legionella pneumophila str. Paris	41.176	97.842	0.403
pilE	Neisseria gonorrhoeae strain FA1090	40.458	94.245	0.381
pilA	Vibrio parahaemolyticus RIMD 2210633	39.695	94.245	0.374
pilE	Neisseria gonorrhoeae MS11	36.957	99.281	0.367

Detailed information NTG0436678