Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HCG51_RS05005 Genome accession   NZ_CP050440
Coordinates   1266618..1267745 (+) Length   375 a.a.
NCBI ID   WP_167719435.1    Uniprot ID   A0A6G9SAD9
Organism   Tolypothrix sp. PCC 7910     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1261618..1272745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCG51_RS04995 (HCG51_04990) grpE 1263391..1264137 (-) 747 WP_167719429.1 nucleotide exchange factor GrpE -
  HCG51_RS05000 (HCG51_04995) pilB/pilB1 1264550..1266565 (+) 2016 WP_167719432.1 GspE/PulE family protein Machinery gene
  HCG51_RS05005 (HCG51_05000) pilT 1266618..1267745 (+) 1128 WP_167719435.1 type IV pilus twitching motility protein PilT Machinery gene
  HCG51_RS05010 (HCG51_05005) - 1267849..1269069 (+) 1221 WP_167719438.1 type II secretion system F family protein -
  HCG51_RS05015 (HCG51_05010) - 1269173..1269730 (+) 558 WP_167719442.1 hypothetical protein -
  HCG51_RS05020 (HCG51_05015) - 1269868..1270890 (-) 1023 WP_167719445.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 40993.36 Da        Isoelectric Point: 7.4775

>NTDB_id=432704 HCG51_RS05005 WP_167719435.1 1266618..1267745(+) (pilT) [Tolypothrix sp. PCC 7910]
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKDRGSYAACLRALSSKIPNFDKLGLPDVVREMTEKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMVITPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEIQRLIGGAPQAAGAKPGANGVAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=432704 HCG51_RS05005 WP_167719435.1 1266618..1267745(+) (pilT) [Tolypothrix sp. PCC 7910]
ATGGAGATGATGATTGAAGACTTGATGGAGCAGATGATTGAAATGGGGGGTTCGGATATGCATTTATCCGCAGGTTTACC
ACCCTATTTCCGGATTAGTGGCAAACTCACTCCCATAGGCGAGCATGTATTGACTGCAGATCAGTGTCAGAGACTGATTT
TTAGTATGCTCAACAATACTCAGCGTAAAACTTTAGAACAGACCTGGGAACTAGATTGTTCTTATGGTGTAAAAGGCTTG
GCTCGGTTCCGCGTCAATGTTTATAAGGATCGTGGTTCCTACGCTGCTTGTTTACGTGCTCTCAGCTCCAAAATTCCTAA
CTTTGACAAATTAGGGTTGCCAGATGTAGTCCGGGAAATGACAGAAAAGCCCAGAGGATTGATTTTGGTGACAGGGCCCA
CTGGTTCTGGTAAGACAACTACCTTGGCGGCAATGATTGACTTGATTAACCGCACCAAGGCAGAGCATATCTTGACGGTG
GAAGATCCAATTGAATTTGTGTATGAACCAATTAAAAGCTTGGTTCACCAGCGACAACTTGGTGAAGATACCAAGAGTTT
TGCTAATGCTTTAAAAGCAGCTTTGCGGGAAGATCCAGATATTATTCTGGTTGGGGAAATGCGGGATTTAGAAACGATTT
CTTTGGCTATTTCTGCTGCGGAAACAGGGCACTTAGTATTTGGAACTTTGCACACCAGTTCTGCTGCACAGACTGTTGAC
CGGATTATTGACGTTTTCCCCCACGAAAGACAAACCCAGGTACGGGTTCAGTTGTCAAACTCTCTAGTAGCTGTGTTTAG
CCAAACTTTAGTACCCAAGAAAAATCCCAAACCAGGTGAATATGGTCGGGTGATGGCTCAAGAAATTATGGTTATTACTC
CTGCTATTTCTAACTTAATTCGAGAAGGGAAAACATCTCAAATTTACTCCGCAATCCAGACTGGGGGTAAGTTGGGTATG
CAAACCCTAGAAAAAGTTTTAGCTGATTTGTATAAAGCGGGAACTATCTCTTTTGAAGCTGCTATGTCTAAAACTTCTAA
GCCTGATGAAATTCAACGTCTCATTGGTGGAGCACCACAAGCAGCAGGAGCAAAACCTGGTGCTAATGGTGTTGCAAAAG
CTCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9SAD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.441

93.067

0.507
  pilT Pseudomonas stutzeri DSM 10701

54.155

93.067

0.504
  pilT Legionella pneumophila strain Lp02

53.026

92.533

0.491
  pilT Legionella pneumophila strain ERS1305867

53.026

92.533

0.491
  pilT Acinetobacter baylyi ADP1

52.45

92.533

0.485
  pilT Acinetobacter baumannii strain A118

52.161

92.533

0.483
  pilT Acinetobacter baumannii D1279779

52.161

92.533

0.483
  pilT Acinetobacter nosocomialis M2

52.161

92.533

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.967

98.4

0.472
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.708

91.2

0.453
  pilT Vibrio cholerae strain A1552

49.708

91.2

0.453
  pilT Neisseria gonorrhoeae MS11

50.147

90.4

0.453
  pilT Neisseria meningitidis 8013

48.424

93.067

0.451
  pilU Acinetobacter baylyi ADP1

37.838

98.667

0.373