Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DSUI_RS02935 Genome accession   NC_016616
Coordinates   600038..601174 (+) Length   378 a.a.
NCBI ID   WP_014235716.1    Uniprot ID   A0A5H2Z0L2
Organism   Azospira oryzae PS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595038..606174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSUI_RS02900 (Dsui_0598) cowN 595192..595494 (-) 303 WP_014235709.1 N(2)-fixation sustaining protein CowN -
  DSUI_RS02905 (Dsui_0599) - 595631..596344 (+) 714 WP_014235710.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -
  DSUI_RS02910 (Dsui_0600) - 596387..596677 (-) 291 WP_014235711.1 DUF167 domain-containing protein -
  DSUI_RS02915 (Dsui_0601) - 596677..597249 (-) 573 WP_014235712.1 YggT family protein -
  DSUI_RS02920 (Dsui_0602) proC 597289..598101 (-) 813 WP_014235713.1 pyrroline-5-carboxylate reductase -
  DSUI_RS02925 (Dsui_0603) - 598115..598819 (-) 705 WP_014235714.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSUI_RS02930 (Dsui_0604) pilT 598978..600021 (+) 1044 WP_014235715.1 type IV pilus twitching motility protein PilT Machinery gene
  DSUI_RS02935 (Dsui_0605) pilU 600038..601174 (+) 1137 WP_014235716.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSUI_RS02940 (Dsui_0606) - 601259..602431 (-) 1173 WP_014235717.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  DSUI_RS02945 (Dsui_0607) - 602443..602859 (-) 417 WP_014235718.1 CopD family protein -
  DSUI_RS02950 (Dsui_0608) - 602853..603758 (-) 906 WP_014235719.1 NAD-dependent epimerase/dehydratase family protein -
  DSUI_RS02955 (Dsui_0609) - 603809..604822 (+) 1014 WP_014235720.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  DSUI_RS02960 (Dsui_0610) - 604844..606016 (+) 1173 WP_014235721.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42070.34 Da        Isoelectric Point: 6.5935

>NTDB_id=43124 DSUI_RS02935 WP_014235716.1 600038..601174(+) (pilU) [Azospira oryzae PS]
MERDQALKFIQDLLRLMVQKKGSDLFITAGFPPAIKVDGKMTPVSNQALTPQHTAELARSIMNDRQAAEFESTKECNFAI
SPAGIGRFRVNAFVQQGRIGVICRTINTTIPRFEDLGLPPVLKDVAMTKRGLVLFVGGTGSGKSTSLAAMIGYRNENSYG
HIITIEDPVEYVHEHKNCIVTQREVGVDTDSWEMALKNTLRQAPDVILIGEIRDRETMEHAVAFAETGHLAMGTLHANST
NQALDRIINFFPEERRQQLLMDLSLNLRGIVSQRLIPLKDGKGRAAAIEVMLNSPLISDLIFKGEVTEIKEIMKKSRELG
MQTFDQSLFDLYEAGRISYEDALRNADSVNDLRLQIKLHGKESKDRDLAAGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=43124 DSUI_RS02935 WP_014235716.1 600038..601174(+) (pilU) [Azospira oryzae PS]
ATGGAAAGAGATCAGGCACTCAAATTCATCCAGGACCTGCTGCGCCTCATGGTGCAGAAGAAGGGCTCGGACCTCTTCAT
CACCGCCGGCTTCCCCCCCGCCATCAAGGTGGACGGCAAGATGACCCCGGTCTCCAACCAGGCCCTGACGCCCCAGCACA
CGGCGGAACTGGCCCGCTCCATCATGAACGACCGGCAGGCGGCGGAATTCGAATCCACCAAGGAGTGCAACTTCGCCATC
TCCCCCGCCGGCATCGGCCGCTTCCGGGTCAATGCCTTCGTCCAGCAGGGCCGCATCGGCGTCATCTGCCGTACCATCAA
CACCACCATTCCCCGTTTCGAGGACCTGGGCCTGCCGCCGGTGCTGAAGGACGTGGCCATGACCAAACGGGGCCTGGTGC
TGTTCGTCGGCGGCACCGGCTCCGGCAAGTCCACCTCCCTGGCGGCCATGATCGGCTACCGCAACGAAAACTCCTACGGC
CACATCATCACCATCGAGGACCCGGTGGAGTACGTGCACGAGCACAAGAACTGCATCGTCACCCAGCGCGAGGTGGGCGT
CGATACGGACTCCTGGGAAATGGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ACCGGGAGACCATGGAGCATGCCGTGGCCTTCGCCGAGACCGGCCACCTGGCCATGGGCACCCTGCACGCCAACTCCACC
AACCAGGCCCTCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGCTGATGGACCTCTCCCTCAACCT
GCGCGGCATCGTCTCCCAGCGCCTCATCCCCCTCAAGGACGGCAAGGGCCGGGCGGCGGCCATCGAGGTCATGCTCAACT
CGCCCCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCACCGAGATCAAGGAGATCATGAAGAAGTCCCGGGAGCTGGGC
ATGCAGACCTTCGACCAGTCCCTCTTCGACCTCTACGAGGCCGGCCGCATCAGCTACGAGGACGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTGCACGGCAAGGAATCGAAGGACCGGGACCTGGCGGCCGGCATCGGCC
ACCTGGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2Z0L2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.928

95.503

0.63
  pilU Acinetobacter baylyi ADP1

60.224

94.444

0.569
  pilU Vibrio cholerae strain A1552

57.478

90.212

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Legionella pneumophila strain Lp02

44.745

88.095

0.394
  pilT Legionella pneumophila strain ERS1305867

44.745

88.095

0.394
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Acinetobacter baylyi ADP1

43.068

89.683

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368

Multiple sequence alignment