Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GTCCBUS3UF5_RS13490 Genome accession   NC_016593
Coordinates   2701519..2702574 (-) Length   351 a.a.
NCBI ID   WP_014196435.1    Uniprot ID   U2WMR4
Organism   Geobacillus thermoleovorans CCB_US3_UF5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2696519..2707574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTCCBUS3UF5_RS13460 (GTCCBUS3UF5_29480) - 2696701..2697420 (-) 720 WP_014196429.1 hypothetical protein -
  GTCCBUS3UF5_RS13465 (GTCCBUS3UF5_29490) - 2697417..2697992 (-) 576 WP_014196430.1 fimbrial protein -
  GTCCBUS3UF5_RS13470 (GTCCBUS3UF5_29500) pilM 2697982..2698920 (-) 939 WP_014196431.1 type IV pilus biogenesis protein PilM -
  GTCCBUS3UF5_RS13475 (GTCCBUS3UF5_29510) - 2698942..2699688 (-) 747 WP_014196432.1 prepilin peptidase -
  GTCCBUS3UF5_RS13480 (GTCCBUS3UF5_29520) - 2699717..2700235 (-) 519 WP_014196433.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GTCCBUS3UF5_RS13485 (GTCCBUS3UF5_29530) - 2700321..2701532 (-) 1212 WP_014196434.1 type II secretion system F family protein -
  GTCCBUS3UF5_RS13490 (GTCCBUS3UF5_29540) pilT 2701519..2702574 (-) 1056 WP_014196435.1 type IV pilus twitching motility protein PilT Machinery gene
  GTCCBUS3UF5_RS13495 (GTCCBUS3UF5_29550) - 2702587..2704251 (-) 1665 WP_014196436.1 GspE/PulE family protein -
  GTCCBUS3UF5_RS13500 (GTCCBUS3UF5_29560) - 2704248..2705618 (-) 1371 WP_014196437.1 VanW family protein -
  GTCCBUS3UF5_RS13505 (GTCCBUS3UF5_29570) - 2705635..2706414 (-) 780 WP_014196438.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38467.46 Da        Isoelectric Point: 6.7270

>NTDB_id=42896 GTCCBUS3UF5_RS13490 WP_014196435.1 2701519..2702574(-) (pilT) [Geobacillus thermoleovorans CCB_US3_UF5]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVNGELRAYGHERLHPEETEQMAKAVIPAPLWQQFQETGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPVRIPTLEELGLPDVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNRHKTGRTAALEILVNNAAVANLIRNEKEHQIINIMQTSRSLGMQTME
MSVKELAADGRIDPLAAEVYLAGERGDDGAV

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=42896 GTCCBUS3UF5_RS13490 WP_014196435.1 2701519..2702574(-) (pilT) [Geobacillus thermoleovorans CCB_US3_UF5]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCGGCCTTTGAATGCCGCGCTTCCGACGTGCATTTGACCGTCGGTGCGCC
GCCGATTTTTCGCGTGAACGGGGAGTTGCGAGCTTACGGCCATGAGCGCCTGCACCCGGAGGAAACAGAGCAAATGGCGA
AAGCCGTCATTCCGGCGCCGCTTTGGCAGCAGTTTCAGGAAACCGGAGAGCTTGATTTTTCGTATGGCATTGCCGGCGTT
TCCCGCTTTCGCTTAAACGTGTTTAAACAGCGGTCATGCGTGTCGCTCGCCGTGCGCCTCATCCCGGTGCGCATCCCGAC
GCTGGAGGAACTTGGCCTGCCGGATGTTTTAAAGCGCATCGCCGAAAAGCCGCACGGACTCGTGCTTGTCACCGGACCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAACATATCATCACGCTG
GAAGATCCAATCGAGTATTTGCATAAACACGGGAAATCGATCATCGACCAACGCGAGGTGGGATTCGACACTGGCAGTTT
TGCCGCCGGATTGCGTGCTGCGCTTCGTCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACAATCC
AAACGGCAATCACCGCCGCGGAAACGGGCCATTTAGTGCTTGGCACGCTTCATACGGCGAGCGCGCCGGCGGCGATTGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCGCAAATCCGGCTGCAACTTGCTTCCGTGCTCGTGGCCATCATCGC
TCAGCGCTTGTTTCCAAACAGACACAAAACCGGGCGGACGGCAGCGCTGGAAATTTTAGTGAACAATGCGGCCGTCGCCA
ACTTAATCCGCAACGAAAAAGAACATCAAATCATCAACATTATGCAAACGAGCCGCAGCCTCGGCATGCAGACGATGGAA
ATGAGCGTTAAGGAGCTTGCAGCCGATGGGCGCATCGATCCGCTGGCGGCTGAAGTGTATTTGGCGGGGGAGCGTGGGGA
CGATGGCGCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2WMR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.994

89.174

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.08

92.877

0.456
  pilT Vibrio cholerae strain A1552

49.08

92.877

0.456
  pilT Neisseria gonorrhoeae MS11

48.182

94.017

0.453
  pilT Neisseria meningitidis 8013

48.182

94.017

0.453
  pilT Pseudomonas aeruginosa PAK

49.226

92.023

0.453
  pilT Acinetobacter baylyi ADP1

48.16

92.877

0.447
  pilT Acinetobacter baumannii strain A118

47.273

94.017

0.444
  pilT Acinetobacter baumannii D1279779

47.273

94.017

0.444
  pilT Pseudomonas stutzeri DSM 10701

47.988

92.023

0.442
  pilT Acinetobacter nosocomialis M2

46.97

94.017

0.442
  pilT Legionella pneumophila strain Lp02

47.962

90.883

0.436
  pilT Legionella pneumophila strain ERS1305867

47.962

90.883

0.436
  pilU Vibrio cholerae strain A1552

41.127

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

44.073

93.732

0.413

Multiple sequence alignment