Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ECWSU1_RS18600 Genome accession   NC_016514
Coordinates   3913465..3914490 (-) Length   341 a.a.
NCBI ID   WP_041162845.1    Uniprot ID   -
Organism   Enterobacter ludwigii     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3908465..3919490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECWSU1_RS18570 (EcWSU1_03743) - 3909366..3909863 (+) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -
  ECWSU1_RS18575 (EcWSU1_03744) endA 3909958..3910665 (+) 708 WP_020883698.1 deoxyribonuclease I -
  ECWSU1_RS18580 (EcWSU1_03745) rsmE 3910718..3911449 (+) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ECWSU1_RS18585 (EcWSU1_03746) gshB 3911489..3912436 (+) 948 WP_020883696.1 glutathione synthase -
  ECWSU1_RS18590 (EcWSU1_03747) - 3912523..3913083 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  ECWSU1_RS18595 (EcWSU1_03748) ruvX 3913083..3913499 (+) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  ECWSU1_RS18600 (EcWSU1_03751) pilT 3913465..3914490 (-) 1026 WP_041162845.1 type IV pilus twitching motility protein PilT Machinery gene
  ECWSU1_RS18605 (EcWSU1_03750) - 3914508..3915209 (+) 702 WP_014171569.1 YggS family pyridoxal phosphate-dependent enzyme -
  ECWSU1_RS18610 (EcWSU1_03752) - 3915231..3915797 (+) 567 WP_014171570.1 YggT family protein -
  ECWSU1_RS18615 (EcWSU1_03753) yggU 3915794..3916090 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  ECWSU1_RS18620 (EcWSU1_03754) - 3916094..3916687 (+) 594 WP_014171572.1 XTP/dITP diphosphatase -
  ECWSU1_RS18625 (EcWSU1_03755) hemW 3916680..3917822 (+) 1143 WP_014171573.1 radical SAM family heme chaperone HemW -
  ECWSU1_RS18630 (EcWSU1_03756) - 3917886..3918248 (+) 363 WP_041162846.1 endonuclease domain-containing protein -
  ECWSU1_RS18635 (EcWSU1_03757) - 3918316..3919032 (-) 717 WP_020883689.1 DUF2884 domain-containing protein -
  ECWSU1_RS18640 (EcWSU1_03758) - 3919089..3919415 (-) 327 WP_020883688.1 YggL family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37091.08 Da        Isoelectric Point: 5.8611

>NTDB_id=42824 ECWSU1_RS18600 WP_041162845.1 3913465..3914490(-) (pilT) [Enterobacter ludwigii]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLSALGVPQVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=42824 ECWSU1_RS18600 WP_041162845.1 3913465..3914490(-) (pilT) [Enterobacter ludwigii]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATCCGGTCGTCTTGAACCCGCGCCGTTTCCGCCTCCCGAAGTCGATACGCTATTAAAGGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTCACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCTCCGCGTTGGG
GGTGCCCCAGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGCTAATTCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGAGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.112

99.12

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

99.12

0.487
  pilT Neisseria meningitidis 8013

48.348

97.654

0.472
  pilT Neisseria gonorrhoeae MS11

46.784

100

0.469
  pilT Legionella pneumophila strain ERS1305867

49.068

94.428

0.463
  pilT Legionella pneumophila strain Lp02

49.068

94.428

0.463
  pilT Acinetobacter baylyi ADP1

46.988

97.361

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.706

95.894

0.457
  pilT Acinetobacter baumannii D1279779

47.706

95.894

0.457
  pilT Acinetobacter baumannii strain A118

47.706

95.894

0.457
  pilT Pseudomonas aeruginosa PAK

47.401

95.894

0.455
  pilT Acinetobacter nosocomialis M2

48.125

93.842

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.031

94.428

0.425
  pilU Pseudomonas stutzeri DSM 10701

40.303

96.774

0.39
  pilU Vibrio cholerae strain A1552

39.441

94.428

0.372

Multiple sequence alignment