Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GTQ41_RS22835 Genome accession   NZ_CP049737
Coordinates   5054532..5054969 (-) Length   145 a.a.
NCBI ID   WP_047296615.1    Uniprot ID   -
Organism   Pseudomonas sp. AN-B15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5049532..5059969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTQ41_RS22820 (GTQ41_22845) - 5050400..5053348 (+) 2949 WP_047296613.1 DUF748 domain-containing protein -
  GTQ41_RS22825 (GTQ41_22850) - 5053348..5053644 (+) 297 WP_041065375.1 DUF2845 domain-containing protein -
  GTQ41_RS22830 (GTQ41_22855) - 5053878..5054234 (-) 357 WP_007963876.1 BON domain-containing protein -
  GTQ41_RS22835 (GTQ41_22860) pilA 5054532..5054969 (-) 438 WP_047296615.1 pilin Machinery gene
  GTQ41_RS22840 (GTQ41_22865) pilB 5055198..5056898 (+) 1701 WP_047296617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTQ41_RS22845 (GTQ41_22870) pilC 5056901..5058118 (+) 1218 WP_047296619.1 type II secretion system F family protein Machinery gene
  GTQ41_RS22850 (GTQ41_22875) pilD 5058120..5058992 (+) 873 WP_047296621.1 A24 family peptidase Machinery gene
  GTQ41_RS22855 (GTQ41_22880) coaE 5058989..5059612 (+) 624 WP_047296622.1 dephospho-CoA kinase -
  GTQ41_RS22860 (GTQ41_22885) yacG 5059609..5059809 (+) 201 WP_007963861.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14570.61 Da        Isoelectric Point: 8.1087

>NTDB_id=427421 GTQ41_RS22835 WP_047296615.1 5054532..5054969(-) (pilA) [Pseudomonas sp. AN-B15]
MNNQKGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDTINQGTAPDITAVNGGTTTTSNCTV
AVTGTAATGAGSISCTLLNAPGPVLGKTITLTRSGAATGNTSGVWTCTTTVNPDYSPKGCTASGT

Nucleotide


Download         Length: 438 bp        

>NTDB_id=427421 GTQ41_RS22835 WP_047296615.1 5054532..5054969(-) (pilA) [Pseudomonas sp. AN-B15]
ATGAACAACCAAAAAGGTTTCACCCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGATTGCCCT
GCCGCAGTATTCGAAGTATCAGGCGCGATCAAAAGTGACTGCGGGGCTGGCGGAGATCAGTGCGATGAAGGTGCCGTTTG
AGGACACCATCAATCAAGGTACCGCTCCGGATATAACCGCTGTGAATGGCGGTACAACCACCACCTCCAACTGTACGGTG
GCGGTTACAGGCACAGCGGCGACGGGAGCGGGCAGCATCAGCTGCACGCTGCTCAACGCGCCGGGACCGGTACTGGGCAA
AACCATCACGCTTACTCGTTCGGGCGCTGCTACGGGTAATACCTCTGGTGTGTGGACCTGTACCACGACAGTCAATCCCG
ATTACTCGCCAAAAGGCTGCACTGCCAGCGGCACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.276

100

0.483
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.552

98.621

0.469
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.484

100

0.448
  pilA Vibrio cholerae strain A1552

42.484

100

0.448
  pilA Vibrio cholerae C6706

42.484

100

0.448
  comP Acinetobacter baylyi ADP1

41.83

100

0.441
  pilA Pseudomonas aeruginosa PAK

40.909

100

0.434
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.972

97.241

0.428
  pilA2 Legionella pneumophila str. Paris

40.714

96.552

0.393
  pilA2 Legionella pneumophila strain ERS1305867

40

96.552

0.386
  pilA Vibrio parahaemolyticus RIMD 2210633

39.007

97.241

0.379
  pilA Ralstonia pseudosolanacearum GMI1000

40.602

91.724

0.372