Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GTQ41_RS22840 Genome accession   NZ_CP049737
Coordinates   5055198..5056898 (+) Length   566 a.a.
NCBI ID   WP_047296617.1    Uniprot ID   A0A2A2PP88
Organism   Pseudomonas sp. AN-B15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5050198..5061898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTQ41_RS22820 (GTQ41_22845) - 5050400..5053348 (+) 2949 WP_047296613.1 DUF748 domain-containing protein -
  GTQ41_RS22825 (GTQ41_22850) - 5053348..5053644 (+) 297 WP_041065375.1 DUF2845 domain-containing protein -
  GTQ41_RS22830 (GTQ41_22855) - 5053878..5054234 (-) 357 WP_007963876.1 BON domain-containing protein -
  GTQ41_RS22835 (GTQ41_22860) pilA 5054532..5054969 (-) 438 WP_047296615.1 pilin Machinery gene
  GTQ41_RS22840 (GTQ41_22865) pilB 5055198..5056898 (+) 1701 WP_047296617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTQ41_RS22845 (GTQ41_22870) pilC 5056901..5058118 (+) 1218 WP_047296619.1 type II secretion system F family protein Machinery gene
  GTQ41_RS22850 (GTQ41_22875) pilD 5058120..5058992 (+) 873 WP_047296621.1 A24 family peptidase Machinery gene
  GTQ41_RS22855 (GTQ41_22880) coaE 5058989..5059612 (+) 624 WP_047296622.1 dephospho-CoA kinase -
  GTQ41_RS22860 (GTQ41_22885) yacG 5059609..5059809 (+) 201 WP_007963861.1 DNA gyrase inhibitor YacG -
  GTQ41_RS22865 (GTQ41_22890) - 5059816..5060031 (-) 216 WP_041065358.1 hypothetical protein -
  GTQ41_RS22870 (GTQ41_22895) - 5060097..5060786 (-) 690 WP_047296624.1 energy-coupling factor ABC transporter permease -
  GTQ41_RS22875 (GTQ41_22900) - 5060783..5061250 (-) 468 WP_047296911.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62560.49 Da        Isoelectric Point: 5.9441

>NTDB_id=427422 GTQ41_RS22840 WP_047296617.1 5055198..5056898(+) (pilB) [Pseudomonas sp. AN-B15]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEDMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCNHCKKPIEIPRETLIKEGFPP
EQIGNFTIYEPVGCDHCNGGYKGRVGIYEVVKNTSELQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=427422 GTQ41_RS22840 WP_047296617.1 5055198..5056898(+) (pilB) [Pseudomonas sp. AN-B15]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCTCACAGGTGG
CCGAGATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCTTGTGGCGCCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACCGAGGCCATCGAGAAATTCTTCGACACCCACGCCAGCGGCCTCGAAGACATGGCCGACGTCGATCTCGACGGC
GTAGACGTCGAGTCCGTCGACGACAGCCGCCAGGACGCCATCGGCGGGCTCGACGCCGATGACGCGCCGGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCAATCAAGAGTGGCTCCTCCGACCTGCACTTCGAACCCTACGAGAAAAATTTCCGGG
TGCGTGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAACCGCCGATCCAGTTGGCCGGGCGGATCGCCGCACGCCTG
AAGGTCATGGCCAGCCTGGATATCTCGGAACGCCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGACCAA
GTCGATCGACTTTCGGGTCAACACCCTGCCGACGCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAGATCGGCATCGATGCATTGGGTTACGAACCGGCGCAGAAAGACCTGTACATGGCGGCGCTCAAGCAGCCGCAGGGC
ATGATTCTGGTGACCGGGCCGACCGGCTCGGGGAAAACCGTGTCGCTGTACACCGGCCTGAATATTCTCAATACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GTCTGGATTTTGCCCAGGCCCTGCGTTCGTTTCTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACTGCCGAAATCGCGATCAAGGCCGCACAGACCGGCCACCTGGTGCTGTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGACCCGCCTGCACAACATGGGCATTCCCGGGTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAAACCGATCGAAATCCCCCGCGAAACGCTGATCAAGGAAGGTTTCCCTCCG
GAACAGATCGGCAATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACCTCCGAGCTGCAGCGGCTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACAGCCAGA
TGCGTCGCGACGGCTTCGACGACCTGCGCACCTCGGGCCTGCACAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2PP88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

51.155

99.47

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

36.738

98.587

0.362