Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G5S61_RS18345 Genome accession   NZ_CP049606
Coordinates   3633602..3634543 (-) Length   313 a.a.
NCBI ID   WP_072038732.1    Uniprot ID   -
Organism   Shigella boydii strain 600080     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3628602..3639543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S61_RS18315 (G5S61_18320) - 3629265..3629762 (+) 498 WP_005051770.1 SprT family zinc-dependent metalloprotease -
  G5S61_RS18320 (G5S61_18325) endA 3629857..3630564 (+) 708 WP_000286517.1 deoxyribonuclease I -
  G5S61_RS18325 (G5S61_18330) rsmE 3630644..3631375 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G5S61_RS18330 (G5S61_18335) gshB 3631388..3632338 (+) 951 WP_000593267.1 glutathione synthase -
  G5S61_RS18335 (G5S61_18340) - 3632447..3633010 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  G5S61_RS18340 (G5S61_18345) ruvX 3633010..3633426 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  G5S61_RS18345 (G5S61_18350) pilT 3633602..3634543 (-) 942 WP_072038732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G5S61_RS18350 (G5S61_18355) yggS 3634561..3635265 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  G5S61_RS18355 (G5S61_18360) yggT 3635283..3635849 (+) 567 WP_001094818.1 osmotic shock tolerance protein YggT -
  G5S61_RS18360 (G5S61_18365) yggU 3635846..3636136 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  G5S61_RS18365 (G5S61_18370) - 3636144..3636737 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  G5S61_RS18370 (G5S61_18375) hemW 3636730..3637866 (+) 1137 WP_032294388.1 radical SAM family heme chaperone HemW -
  G5S61_RS18375 (G5S61_18380) - 3638180..3639143 (+) 964 Protein_3648 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34614.57 Da        Isoelectric Point: 5.2305

>NTDB_id=426455 G5S61_RS18345 WP_072038732.1 3633602..3634543(-) (pilT) [Shigella boydii strain 600080]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETAQAVERLVDSFPAQEKDPVRNQL
AGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=426455 G5S61_RS18345 WP_072038732.1 3633602..3634543(-) (pilT) [Shigella boydii strain 600080]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGCGCAGGCAGTTGAGCGACTGGTGGATTCATTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTT
GCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGTGGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACT
GATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGAAAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGC
AGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGGGTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.789

100

0.489
  pilT Vibrio cholerae strain A1552

46.789

100

0.489
  pilT Neisseria meningitidis 8013

45.732

100

0.479
  pilT Neisseria gonorrhoeae MS11

45.427

100

0.476
  pilT Acinetobacter baylyi ADP1

44.037

100

0.46
  pilT Acinetobacter nosocomialis M2

43.731

100

0.457
  pilT Acinetobacter baumannii D1279779

43.731

100

0.457
  pilT Acinetobacter baumannii strain A118

43.731

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

43.731

100

0.457
  pilT Pseudomonas aeruginosa PAK

43.425

100

0.454
  pilT Legionella pneumophila strain ERS1305867

42.508

100

0.444
  pilT Legionella pneumophila strain Lp02

42.508

100

0.444
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.308

100

0.399
  pilU Vibrio cholerae strain A1552

37.082

100

0.39
  pilU Pseudomonas stutzeri DSM 10701

34.848

100

0.367