Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G5577_RS09495 Genome accession   NZ_CP049192
Coordinates   2024989..2025969 (-) Length   326 a.a.
NCBI ID   WP_164474552.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain Y2152     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2019989..2030969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5577_RS09465 (G5577_09470) - 2020866..2021363 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  G5577_RS09470 (G5577_09475) endA 2021458..2022165 (+) 708 WP_164474551.1 deoxyribonuclease I -
  G5577_RS09475 (G5577_09480) rsmE 2022217..2022948 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G5577_RS09480 (G5577_09485) gshB 2022980..2023927 (+) 948 WP_003860032.1 glutathione synthase -
  G5577_RS09485 (G5577_09490) - 2024002..2024562 (+) 561 WP_003860031.1 YqgE/AlgH family protein -
  G5577_RS09490 (G5577_09495) ruvX 2024562..2024978 (+) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  G5577_RS09495 (G5577_09500) pilT 2024989..2025969 (-) 981 WP_164474552.1 type IV pilus twitching motility protein PilT Machinery gene
  G5577_RS09500 (G5577_09505) - 2025987..2026685 (+) 699 WP_023314824.1 YggS family pyridoxal phosphate-dependent enzyme -
  G5577_RS09505 (G5577_09510) - 2026710..2027276 (+) 567 WP_003860024.1 YggT family protein -
  G5577_RS09510 (G5577_09515) yggU 2027273..2027569 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  G5577_RS09515 (G5577_09520) - 2027573..2028166 (+) 594 WP_003860021.1 XTP/dITP diphosphatase -
  G5577_RS09520 (G5577_09525) hemW 2028159..2029307 (+) 1149 WP_045332868.1 radical SAM family heme chaperone HemW -
  G5577_RS09525 (G5577_09530) - 2029364..2029726 (+) 363 WP_137048429.1 DUF559 domain-containing protein -
  G5577_RS09530 (G5577_09535) - 2029793..2030509 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  G5577_RS09535 (G5577_09540) - 2030567..2030893 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35636.63 Da        Isoelectric Point: 6.2335

>NTDB_id=425345 G5577_RS09495 WP_164474552.1 2024989..2025969(-) (pilT) [Enterobacter hormaechei strain Y2152]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQAGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=425345 G5577_RS09495 WP_164474552.1 2024989..2025969(-) (pilT) [Enterobacter hormaechei strain Y2152]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTTCTCAATCACCAGACGGACGGGCACATTCTGACCCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTAATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAACGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCTTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGCCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377