Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ENTAS_RS18305 Genome accession   NC_015968
Coordinates   3892645..3893652 (-) Length   335 a.a.
NCBI ID   WP_041162122.1    Uniprot ID   -
Organism   Enterobacter soli     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3887645..3898652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENTAS_RS18270 (Entas_3614) - 3887767..3888264 (+) 498 WP_014071691.1 SprT family zinc-dependent metalloprotease -
  ENTAS_RS18275 (Entas_3615) endA 3888359..3889066 (+) 708 WP_014071692.1 deoxyribonuclease I -
  ENTAS_RS18280 (Entas_3616) rsmE 3889119..3889850 (+) 732 WP_014071693.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ENTAS_RS18285 (Entas_3617) gshB 3889870..3890817 (+) 948 WP_014071694.1 glutathione synthase -
  ENTAS_RS18290 (Entas_3618) - 3890892..3891452 (+) 561 WP_014071695.1 YqgE/AlgH family protein -
  ENTAS_RS18295 (Entas_3619) ruvX 3891452..3891868 (+) 417 WP_014071696.1 Holliday junction resolvase RuvX -
  ENTAS_RS18300 (Entas_3620) - 3891862..3892584 (-) 723 WP_014071697.1 IclR family transcriptional regulator -
  ENTAS_RS18305 (Entas_3621) pilT 3892645..3893652 (-) 1008 WP_041162122.1 type IV pilus twitching motility protein PilT Machinery gene
  ENTAS_RS18310 (Entas_3622) - 3893670..3894374 (+) 705 WP_014071699.1 YggS family pyridoxal phosphate-dependent enzyme -
  ENTAS_RS18315 (Entas_3623) - 3894393..3894959 (+) 567 WP_014071700.1 YggT family protein -
  ENTAS_RS18320 (Entas_3624) yggU 3894956..3895252 (+) 297 WP_014071701.1 DUF167 family protein YggU -
  ENTAS_RS18325 (Entas_3625) - 3895256..3895849 (+) 594 WP_014071702.1 XTP/dITP diphosphatase -
  ENTAS_RS18330 (Entas_3626) hemW 3895842..3896990 (+) 1149 WP_014071703.1 radical SAM family heme chaperone HemW -
  ENTAS_RS18335 (Entas_3627) - 3897047..3897409 (+) 363 WP_014071704.1 endonuclease domain-containing protein -
  ENTAS_RS18340 (Entas_3628) - 3897470..3898189 (-) 720 WP_014071705.1 DUF2884 domain-containing protein -
  ENTAS_RS18345 (Entas_3629) - 3898243..3898569 (-) 327 WP_014071706.1 YggL family protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36728.85 Da        Isoelectric Point: 6.8754

>NTDB_id=42276 ENTAS_RS18305 WP_041162122.1 3892645..3893652(-) (pilT) [Enterobacter soli]
MVMEEMVALSVKHNVSDLHLCSDSPPRWRRAGTLEPAPFPPPDITQLLKTWLNDEQQGTWWANGQVDFAVTLASQQRLRG
SAFSHINGTSLTLRLLPANCPQLSALGAPRTLPVLLDKDSGLILVTGATGSGKSTTLAAMVDHLNRHYDKHILTLEDPVE
FIYQSDRCLIHQREIGQHCPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLCRDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSFAER
RAQGRLHERSVVASG

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=42276 ENTAS_RS18305 WP_041162122.1 3892645..3893652(-) (pilT) [Enterobacter soli]
GTGGTGATGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTGATTCACCGCCACG
CTGGCGCAGGGCTGGTACTCTTGAACCTGCGCCATTCCCGCCGCCGGATATTACGCAGCTATTAAAAACGTGGCTTAACG
ACGAACAACAGGGGACCTGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGCTCGCCAGCCAGCAGCGTTTACGCGGC
AGCGCATTCAGCCACATTAACGGTACCTCACTCACGCTCAGACTCTTGCCAGCAAACTGCCCGCAGCTTTCGGCGCTGGG
GGCGCCGCGTACGCTCCCGGTACTGCTGGACAAAGACAGCGGTCTGATTCTGGTGACGGGAGCAACCGGAAGTGGCAAAT
CCACTACGCTTGCGGCAATGGTGGACCATCTTAACCGCCACTACGATAAACACATTTTGACGCTGGAAGACCCGGTTGAG
TTTATTTATCAAAGCGATCGCTGTCTTATCCATCAGCGGGAGATAGGTCAGCACTGCCCTTCATTTGCAGAGGCGCTTCG
CGCCGCATTACGTGAAGATCCTGACGTGATTTTATTAGGGGAGCTTCGCGACAGCGAAACGATCAGGCTGGCCCTTACGG
CGGCGGAGACTGGCCATCTGGTACTGGCAACGCTGCATACGCGTGGGGCAGCGCAGGCAATAGAGCGCCTGGTCGATACC
TTTCCGGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTCTGCGCGCAGTGCTGGCGCAAAAGCTTTGTCG
GGACGTCCAGGGAGGACGCGTGGCGCTGTACGAACTGCTGGTGAATACCTCAGCGGCAGCAAACCTTATCCGCGAGGGCA
AAACCTGGCAGTTGCCCGGTATTATCCAGACCGGGCAACAGGCAGGGATGCAGAATTTTGACCAAAGCTTTGCTGAGCGC
AGAGCGCAGGGGCGGCTTCACGAAAGGTCAGTCGTTGCGTCGGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

97.91

0.493
  pilT Vibrio cholerae strain A1552

50.305

97.91

0.493
  pilT Pseudomonas stutzeri DSM 10701

47.095

97.612

0.46
  pilT Neisseria meningitidis 8013

46.951

97.91

0.46
  pilT Neisseria gonorrhoeae MS11

46.646

97.91

0.457
  pilT Legionella pneumophila strain ERS1305867

47.205

96.119

0.454
  pilT Legionella pneumophila strain Lp02

47.205

96.119

0.454
  pilT Acinetobacter baumannii strain A118

46.483

97.612

0.454
  pilT Acinetobacter baylyi ADP1

46.483

97.612

0.454
  pilT Acinetobacter baumannii D1279779

46.483

97.612

0.454
  pilT Pseudomonas aeruginosa PAK

46.177

97.612

0.451
  pilT Acinetobacter nosocomialis M2

46.875

95.522

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.188

95.522

0.403
  pilU Pseudomonas stutzeri DSM 10701

38.788

98.507

0.382
  pilU Vibrio cholerae strain A1552

38.037

97.313

0.37

Multiple sequence alignment