Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G0034_RS18995 Genome accession   NZ_CP048736
Coordinates   3969720..3970700 (-) Length   326 a.a.
NCBI ID   WP_071922901.1    Uniprot ID   -
Organism   Enterobacter sp. T2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3964720..3975700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0034_RS18965 (G0034_18965) - 3965609..3966106 (+) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -
  G0034_RS18970 (G0034_18970) endA 3966201..3966908 (+) 708 WP_014885187.1 deoxyribonuclease I -
  G0034_RS18975 (G0034_18975) rsmE 3966960..3967691 (+) 732 WP_014885188.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G0034_RS18980 (G0034_18980) gshB 3967711..3968658 (+) 948 WP_023331278.1 glutathione synthase -
  G0034_RS18985 (G0034_18985) - 3968733..3969293 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  G0034_RS18990 (G0034_18990) ruvX 3969293..3969709 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  G0034_RS18995 (G0034_18995) pilT 3969720..3970700 (-) 981 WP_071922901.1 type IV pilus twitching motility protein PilT Machinery gene
  G0034_RS19000 (G0034_19000) - 3970718..3971419 (+) 702 WP_045134813.1 YggS family pyridoxal phosphate-dependent enzyme -
  G0034_RS19005 (G0034_19005) - 3971441..3972007 (+) 567 WP_014885192.1 YggT family protein -
  G0034_RS19010 (G0034_19010) yggU 3972004..3972300 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  G0034_RS19015 (G0034_19015) - 3972304..3972897 (+) 594 WP_023331280.1 XTP/dITP diphosphatase -
  G0034_RS19020 (G0034_19020) hemW 3972890..3974032 (+) 1143 WP_014885195.1 radical SAM family heme chaperone HemW -
  G0034_RS19025 (G0034_19025) - 3974096..3974434 (+) 339 WP_179121193.1 endonuclease domain-containing protein -
  G0034_RS19030 (G0034_19030) - 3974516..3975232 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  G0034_RS19035 (G0034_19035) - 3975290..3975616 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35576.54 Da        Isoelectric Point: 6.4110

>NTDB_id=422603 G0034_RS18995 WP_071922901.1 3969720..3970700(-) (pilT) [Enterobacter sp. T2]
MDVEEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIDRLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=422603 G0034_RS18995 WP_071922901.1 3969720..3970700(-) (pilT) [Enterobacter sp. T2]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCTGATGTCGGGGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTTTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTCCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAGATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGATCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTTATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAAAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.334

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment