Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GVI76_RS20250 Genome accession   NZ_CP048696
Coordinates   4165130..4166110 (-) Length   326 a.a.
NCBI ID   WP_032619064.1    Uniprot ID   A0AA37AL66
Organism   Enterobacter hormaechei strain Eho-E4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4160130..4171110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI76_RS20220 (GVI76_20215) - 4161008..4161505 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  GVI76_RS20225 (GVI76_20220) endA 4161600..4162307 (+) 708 WP_003860034.1 deoxyribonuclease I -
  GVI76_RS20230 (GVI76_20225) rsmE 4162359..4163090 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GVI76_RS20235 (GVI76_20230) gshB 4163122..4164069 (+) 948 WP_015571835.1 glutathione synthase -
  GVI76_RS20240 (GVI76_20235) - 4164144..4164704 (+) 561 WP_032619065.1 YqgE/AlgH family protein -
  GVI76_RS20245 (GVI76_20240) ruvX 4164704..4165120 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  GVI76_RS20250 (GVI76_20245) pilT 4165130..4166110 (-) 981 WP_032619064.1 type IV pilus twitching motility protein PilT Machinery gene
  GVI76_RS20255 (GVI76_20250) - 4166128..4166829 (+) 702 WP_017692690.1 YggS family pyridoxal phosphate-dependent enzyme -
  GVI76_RS20260 (GVI76_20255) - 4166851..4167417 (+) 567 WP_015571838.1 YggT family protein -
  GVI76_RS20265 (GVI76_20260) yggU 4167414..4167710 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  GVI76_RS20270 (GVI76_20265) - 4167714..4168307 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  GVI76_RS20275 (GVI76_20270) hemW 4168300..4169448 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  GVI76_RS20280 (GVI76_20275) - 4169505..4169843 (+) 339 WP_023304405.1 endonuclease domain-containing protein -
  GVI76_RS20285 (GVI76_20280) - 4169927..4170643 (-) 717 WP_023304406.1 DUF2884 domain-containing protein -
  GVI76_RS20290 (GVI76_20285) - 4170701..4171027 (-) 327 WP_032619063.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35706.68 Da        Isoelectric Point: 6.4131

>NTDB_id=422438 GVI76_RS20250 WP_032619064.1 4165130..4166110(-) (pilT) [Enterobacter hormaechei strain Eho-E4]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=422438 GVI76_RS20250 WP_032619064.1 4165130..4166110(-) (pilT) [Enterobacter hormaechei strain Eho-E4]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCTCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment