Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GUY47_RS20070 Genome accession   NZ_CP047965
Coordinates   4164473..4165453 (-) Length   326 a.a.
NCBI ID   WP_017382924.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 189     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4159473..4170453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUY47_RS20040 (GUY47_20030) - 4160350..4160847 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  GUY47_RS20045 (GUY47_20035) endA 4160942..4161649 (+) 708 WP_003860034.1 deoxyribonuclease I -
  GUY47_RS20050 (GUY47_20040) rsmE 4161701..4162432 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GUY47_RS20055 (GUY47_20045) gshB 4162464..4163411 (+) 948 WP_015571835.1 glutathione synthase -
  GUY47_RS20060 (GUY47_20050) - 4163486..4164046 (+) 561 WP_032654982.1 YqgE/AlgH family protein -
  GUY47_RS20065 (GUY47_20055) ruvX 4164046..4164462 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  GUY47_RS20070 (GUY47_20060) pilT 4164473..4165453 (-) 981 WP_017382924.1 type IV pilus twitching motility protein PilT Machinery gene
  GUY47_RS20075 (GUY47_20065) - 4165471..4166172 (+) 702 WP_017382925.1 YggS family pyridoxal phosphate-dependent enzyme -
  GUY47_RS20080 (GUY47_20070) - 4166194..4166760 (+) 567 WP_015571838.1 YggT family protein -
  GUY47_RS20085 (GUY47_20075) yggU 4166757..4167053 (+) 297 WP_032654984.1 DUF167 family protein YggU -
  GUY47_RS20090 (GUY47_20080) - 4167057..4167650 (+) 594 WP_032654987.1 XTP/dITP diphosphatase -
  GUY47_RS20095 (GUY47_20085) hemW 4167643..4168791 (+) 1149 WP_032654990.1 radical SAM family heme chaperone HemW -
  GUY47_RS20100 (GUY47_20090) - 4168848..4169186 (+) 339 WP_032654993.1 endonuclease domain-containing protein -
  GUY47_RS20105 (GUY47_20095) - 4169270..4169986 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  GUY47_RS20110 (GUY47_20100) - 4170044..4170370 (-) 327 WP_023304407.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35665.59 Da        Isoelectric Point: 6.0405

>NTDB_id=418823 GUY47_RS20070 WP_017382924.1 4164473..4165453(-) (pilT) [Enterobacter hormaechei strain 189]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=418823 GUY47_RS20070 WP_017382924.1 4164473..4165453(-) (pilT) [Enterobacter hormaechei strain 189]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATTGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCCAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377

Multiple sequence alignment