Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynRS9907_RS00095 Genome accession   NZ_CP047944
Coordinates   21574..22650 (-) Length   358 a.a.
NCBI ID   WP_188021419.1    Uniprot ID   A0A7G8FH60
Organism   Synechococcus sp. RS9907     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16574..27650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynRS9907_RS00070 (SynRS9907_00014) - 17015..17527 (+) 513 WP_188021414.1 RNA-binding protein -
  SynRS9907_RS00075 (SynRS9907_00015) dusA 17541..18536 (-) 996 WP_188021415.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynRS9907_RS00080 (SynRS9907_00016) msrB 18577..19080 (+) 504 WP_188021416.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynRS9907_RS00085 (SynRS9907_00017) - 19052..20320 (+) 1269 WP_188021417.1 NAD(P)/FAD-dependent oxidoreductase -
  SynRS9907_RS00090 (SynRS9907_00018) - 20295..21554 (-) 1260 WP_188021418.1 type II secretion system F family protein -
  SynRS9907_RS00095 (SynRS9907_00019) pilT 21574..22650 (-) 1077 WP_188021419.1 type IV pilus twitching motility protein PilT Machinery gene
  SynRS9907_RS00100 (SynRS9907_00020) - 22661..24511 (-) 1851 WP_188021420.1 GspE/PulE family protein -
  SynRS9907_RS00105 (SynRS9907_00021) grpE 24602..25279 (+) 678 WP_188021421.1 nucleotide exchange factor GrpE -
  SynRS9907_RS00110 (SynRS9907_00022) dnaJ 25279..26415 (+) 1137 WP_114988830.1 molecular chaperone DnaJ -
  SynRS9907_RS00115 (SynRS9907_00023) - 26412..26639 (+) 228 WP_114988831.1 sulfurtransferase TusA family protein -
  SynRS9907_RS00120 (SynRS9907_00024) rsgA 26665..27546 (+) 882 WP_188022290.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39189.61 Da        Isoelectric Point: 5.2573

>NTDB_id=418625 SynRS9907_RS00095 WP_188021419.1 21574..22650(-) (pilT) [Synechococcus sp. RS9907]
MDLMIEDLMEQLVQGGGSDLHLATGQPPYGRFSGELRPMTDQPLAEEDCNKLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSVELLNLPPVVLETSARPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYQSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAVSAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFSQTLCRRQNPAEGQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGEQGM
QTLEKALANLVLNGDVSRAEAMGKASKPGELQRLIGEI

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418625 SynRS9907_RS00095 WP_188021419.1 21574..22650(-) (pilT) [Synechococcus sp. RS9907]
ATGGATCTGATGATCGAAGACCTGATGGAACAACTGGTGCAGGGCGGTGGCAGTGATCTGCACCTCGCCACCGGTCAACC
GCCCTACGGCCGCTTCAGCGGTGAACTGCGTCCGATGACGGACCAACCCCTGGCCGAGGAGGACTGCAACAAGCTGATCT
TCTCGATGTTGAACAACAGCCAGCGCAAGACGCTGGAGCAGACCTGGGAACTGGACTGCGCCTACGGCCTCAAGGGTGTT
GCACGCTTCCGCGTCAACGTCTACCGCCAGAAAGGCAGCTATGCCGCCTGCCTGCGGGCCCTTGGCAGCACGATTCCCAG
CGTTGAACTGCTGAACCTTCCTCCTGTTGTGCTGGAGACCAGCGCGCGGCCCCGGGGGCTTGTGCTGGTGACTGGTCCGA
CCGGATCCGGCAAGACCACCACCCTGGCGGCGCTGCTGGATCACATCAATCACACCCGCAGCGAACACATCCTCACGATC
GAAGACCCGATCGAGTTCGTGTACCAGAGCGACAAAAGCCTGGTGCACCAACGCCAGCTGAACGAAGACACCCGCAGCTT
CGCCAACGCCCTACGCGCCGCCCTGCGGGAGGACCCGGACGTGATCCTGGTGGGTGAAATGCGGGATCTGGAGACCATTC
AACTGGCGGTGAGTGCCGCCGAAACCGGACATCTCGTCTTCGGAACCCTGCACACGAGCTCCGCCGCCCAGACCGTGGAC
CGCATGGTCGACGTCTTCCCACCGGAACAACAGACGCAGATCCGTGTGCAGCTCTCAGGCAGTTTGGTCGCTGTGTTCTC
CCAGACCCTCTGCCGACGTCAGAACCCGGCAGAAGGACAGTTCGGTCGGGTGATGGCCCAAGAGATCCTGATCAACACAC
CCGCCACCGCCAACCTGATCCGCGAGGGAAAAACAGCCCAGCTCTATTCCCAAATCCAGACGGGGGGAGAACAGGGCATG
CAGACCCTGGAGAAAGCCTTGGCCAACCTTGTGCTCAACGGGGATGTGTCCCGCGCTGAAGCGATGGGCAAAGCCAGCAA
ACCAGGCGAATTGCAGCGCTTGATCGGTGAAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8FH60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.07

96.089

0.52
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.089

0.511
  pilT Legionella pneumophila strain Lp02

52.819

94.134

0.497
  pilT Legionella pneumophila strain ERS1305867

52.819

94.134

0.497
  pilT Acinetobacter baumannii D1279779

51.744

96.089

0.497
  pilT Acinetobacter baumannii strain A118

51.744

96.089

0.497
  pilT Acinetobacter nosocomialis M2

51.744

96.089

0.497
  pilT Neisseria meningitidis 8013

51.149

97.207

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.571

97.765

0.494
  pilT Acinetobacter baylyi ADP1

50.872

96.089

0.489
  pilT Neisseria gonorrhoeae MS11

51.02

95.81

0.489
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilU Pseudomonas stutzeri DSM 10701

38.462

98.045

0.377
  pilU Vibrio cholerae strain A1552

38.348

94.693

0.363

Multiple sequence alignment