Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CyaNS01_RS00090 Genome accession   NZ_CP047940
Coordinates   21652..22731 (-) Length   359 a.a.
NCBI ID   WP_186697881.1    Uniprot ID   A0A7G8EIP2
Organism   Cyanobium sp. NS01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16652..27731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CyaNS01_RS00070 (CyaNS01_00014) - 17504..17959 (+) 456 WP_186697874.1 RNA-binding protein -
  CyaNS01_RS00075 (CyaNS01_00015) dusA 17991..19016 (-) 1026 WP_186697876.1 tRNA dihydrouridine(20/20a) synthase DusA -
  CyaNS01_RS00080 (CyaNS01_00016) - 19147..20370 (+) 1224 WP_255460157.1 NAD(P)/FAD-dependent oxidoreductase -
  CyaNS01_RS00085 (CyaNS01_00017) - 20381..21637 (-) 1257 WP_186697880.1 type II secretion system F family protein -
  CyaNS01_RS00090 (CyaNS01_00018) pilT 21652..22731 (-) 1080 WP_186697881.1 type IV pilus twitching motility protein PilT Machinery gene
  CyaNS01_RS00095 (CyaNS01_00019) pilB/pilB1 22808..24658 (-) 1851 WP_186697883.1 GspE/PulE family protein Machinery gene
  CyaNS01_RS00100 (CyaNS01_00020) grpE 24757..25527 (+) 771 WP_225875708.1 nucleotide exchange factor GrpE -
  CyaNS01_RS00105 (CyaNS01_00021) dnaJ 25530..26654 (+) 1125 WP_186697885.1 molecular chaperone DnaJ -
  CyaNS01_RS00110 (CyaNS01_00022) - 26658..26912 (+) 255 WP_186697887.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39683.41 Da        Isoelectric Point: 5.9317

>NTDB_id=418581 CyaNS01_RS00090 WP_186697881.1 21652..22731(-) (pilT) [Cyanobium sp. NS01]
MAVMIEDLMTELVAAGGSDLHISSGLPPYGRFNGQLRPIVEERLDEEDCNKLIFSILNNSQRKRLEQDWELDCSYGLRGV
ARFRINVYRQRGTYAASLRALGSTIPSLESLNMPSIVEEMSRSPKGLVLVTGPTGSGKTTTLAAILNHINQTRTEHILTI
EDPIEFTYSSDKSMIHQRQLNDDTRSFSNALRSALREDPDVILVGEMRDLETIQLAITAAETGHLVLASLHTSSASQTVD
RMVDVFPAAQQSQVRVQLSGSLVAVFAQTLCKRHKPMPNQLGRVMAQEIMISTPAIANLIREGKTAQLYSQIQMGGELAM
QTLEKSLADLVLSNNILIDEAITKTSRPEELKRLLNQLG

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=418581 CyaNS01_RS00090 WP_186697881.1 21652..22731(-) (pilT) [Cyanobium sp. NS01]
ATGGCCGTGATGATCGAAGATCTGATGACTGAGCTGGTCGCTGCTGGTGGCAGTGACCTCCACATCAGTTCCGGGCTACC
GCCCTATGGGCGCTTCAACGGGCAGTTGAGACCCATCGTTGAGGAACGACTCGATGAGGAGGACTGCAACAAGCTGATTT
TCTCGATTCTCAATAATTCCCAGCGCAAGCGTCTTGAGCAAGACTGGGAACTTGACTGTTCCTACGGCCTCAGGGGTGTG
GCCAGGTTCAGGATCAATGTCTACCGGCAGCGCGGCACCTATGCCGCCTCTCTGAGGGCCCTGGGTAGCACCATACCCTC
GCTGGAGAGTCTGAACATGCCATCCATCGTCGAGGAGATGAGTCGTTCCCCTAAGGGTCTTGTACTCGTCACCGGGCCCA
CGGGATCAGGCAAGACCACCACCCTGGCAGCCATCCTGAATCACATCAATCAAACTCGCACTGAACATATTCTCACCATT
GAAGACCCGATTGAATTCACCTATTCTTCAGACAAGAGCATGATCCATCAGCGTCAGCTGAATGACGACACCCGCAGCTT
CTCCAATGCGCTGAGGTCAGCACTACGGGAAGATCCCGATGTGATTCTCGTTGGAGAAATGCGTGACCTTGAGACCATTC
AGCTGGCCATCACTGCCGCTGAAACAGGGCATTTGGTGCTCGCTTCGCTGCACACGAGTTCGGCCTCTCAGACCGTGGAC
CGCATGGTGGATGTGTTCCCCGCTGCCCAGCAGAGCCAGGTCAGGGTTCAGCTCAGCGGCAGCCTCGTAGCGGTGTTTGC
TCAGACCCTGTGTAAACGCCACAAGCCAATGCCCAATCAGCTGGGTAGGGTCATGGCCCAGGAGATCATGATCAGCACTC
CGGCCATCGCCAACCTGATCCGCGAAGGGAAAACAGCTCAGCTCTACTCCCAGATTCAGATGGGTGGTGAACTGGCCATG
CAGACTCTGGAGAAGTCCCTGGCTGATCTGGTGCTCAGCAACAACATTCTGATCGATGAAGCCATCACCAAAACCAGTCG
ACCCGAAGAGCTCAAGCGACTGCTGAACCAACTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8EIP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.602

96.657

0.518
  pilT Pseudomonas aeruginosa PAK

53.026

96.657

0.513
  pilT Legionella pneumophila strain Lp02

53.916

92.479

0.499
  pilT Legionella pneumophila strain ERS1305867

53.916

92.479

0.499
  pilT Acinetobacter baumannii D1279779

50.72

96.657

0.49
  pilT Acinetobacter baumannii strain A118

50.72

96.657

0.49
  pilT Acinetobacter nosocomialis M2

50.72

96.657

0.49
  pilT Neisseria meningitidis 8013

53.517

91.086

0.487
  pilT Acinetobacter baylyi ADP1

50.432

96.657

0.487
  pilT Neisseria gonorrhoeae MS11

53.211

91.086

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.294

91.086

0.476
  pilT Vibrio cholerae strain A1552

52.294

91.086

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.591

98.05

0.457
  pilU Pseudomonas stutzeri DSM 10701

37.183

98.886

0.368

Multiple sequence alignment