Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CyaNS01_RS00095 Genome accession   NZ_CP047940
Coordinates   22808..24658 (-) Length   616 a.a.
NCBI ID   WP_186697883.1    Uniprot ID   A0A7G8EIP3
Organism   Cyanobium sp. NS01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17808..29658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CyaNS01_RS00075 (CyaNS01_00015) dusA 17991..19016 (-) 1026 WP_186697876.1 tRNA dihydrouridine(20/20a) synthase DusA -
  CyaNS01_RS00080 (CyaNS01_00016) - 19147..20370 (+) 1224 WP_255460157.1 NAD(P)/FAD-dependent oxidoreductase -
  CyaNS01_RS00085 (CyaNS01_00017) - 20381..21637 (-) 1257 WP_186697880.1 type II secretion system F family protein -
  CyaNS01_RS00090 (CyaNS01_00018) pilT 21652..22731 (-) 1080 WP_186697881.1 type IV pilus twitching motility protein PilT Machinery gene
  CyaNS01_RS00095 (CyaNS01_00019) pilB/pilB1 22808..24658 (-) 1851 WP_186697883.1 GspE/PulE family protein Machinery gene
  CyaNS01_RS00100 (CyaNS01_00020) grpE 24757..25527 (+) 771 WP_225875708.1 nucleotide exchange factor GrpE -
  CyaNS01_RS00105 (CyaNS01_00021) dnaJ 25530..26654 (+) 1125 WP_186697885.1 molecular chaperone DnaJ -
  CyaNS01_RS00110 (CyaNS01_00022) - 26658..26912 (+) 255 WP_186697887.1 sulfurtransferase TusA family protein -
  CyaNS01_RS00115 (CyaNS01_00023) rsgA 26902..27963 (+) 1062 WP_186697888.1 ribosome small subunit-dependent GTPase A -
  CyaNS01_RS00120 (CyaNS01_00024) - 27969..28310 (-) 342 WP_186697890.1 YbaB/EbfC family nucleoid-associated protein -
  CyaNS01_RS00125 (CyaNS01_00025) murB 28356..29261 (-) 906 WP_186697892.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 616 a.a.        Molecular weight: 67467.61 Da        Isoelectric Point: 4.7092

>NTDB_id=418582 CyaNS01_RS00095 WP_186697883.1 22808..24658(-) (pilB/pilB1) [Cyanobium sp. NS01]
MPLRPFPPFPPLPPGPLPGDHSAAGGDQAAAGLLQQPLLDRGDLTPEEVRNPGLSFERWGEFGACPIAERPGVLVVAVPS
DWGREPRRAVQLALSALGLEADLLQARSRDITDALEQMALLTSLEQEESSRETAGKDEASDLMEPRSLTKDLELPSASGQ
LQEVLELEDLEVSEAETNSSANASPIVSLVDRILIEALSIGASDIHVEPYDQTLEIRFRIDGVLQKLFEDLPRSLVPAVT
SRLKIMAELDIAERRLPQDGRIRRLFRGRKMDFRVSTLPTRNGEKVVLRLLDSSSTELGLDTLITNPDARATLRELGSNP
YGMVLVTGPTGSGKSTTLYALLSERNNPDINISTVEDPIEYTLQGITQTQVNRDKGLDFPTTLRAFMRQDPDVLLVGETR
DLETAKTAIEAALTGHLVFTTLHCNDAASAITRLDEMGVEPFMVSASLLGIVSQRLVRRVCSQCRISYHPDPEELSRFGL
LVSEVSDVTFYRADTGHGRQKRACPNCNGNGYKGRVAAFEVLRINDAISTAIAQRQTTDVIRRLALESGMKTLLGYGLEL
VREGHTTLAEVQRILLTDTGLESERRSRALSTLTCKDCGAGLSDAWLECPYCLTPR

Nucleotide


Download         Length: 1851 bp        

>NTDB_id=418582 CyaNS01_RS00095 WP_186697883.1 22808..24658(-) (pilB/pilB1) [Cyanobium sp. NS01]
ATGCCGCTCAGACCGTTTCCCCCCTTCCCCCCGCTGCCTCCAGGGCCCCTGCCTGGAGACCACAGCGCTGCTGGAGGTGA
TCAGGCCGCCGCAGGGCTGCTGCAACAACCTCTGCTCGATCGGGGAGACCTCACGCCGGAGGAGGTACGCAATCCCGGCC
TGAGCTTCGAGCGCTGGGGCGAGTTTGGCGCCTGTCCCATCGCCGAACGCCCCGGAGTGCTGGTGGTGGCCGTACCGAGC
GACTGGGGCCGCGAACCGCGGAGGGCCGTGCAGCTTGCCTTGAGCGCGCTGGGCCTGGAGGCGGACTTACTCCAGGCCAG
GAGCAGGGATATCACCGATGCCCTCGAGCAAATGGCCCTGCTGACCTCCCTGGAACAGGAGGAGTCGTCTCGTGAAACGG
CTGGGAAGGACGAGGCCAGCGACCTGATGGAGCCACGCTCGCTCACGAAGGATCTTGAGCTTCCCAGCGCCAGCGGTCAG
CTGCAAGAGGTGCTGGAACTGGAAGACCTGGAGGTCAGCGAAGCCGAAACCAACTCCAGCGCCAATGCCTCACCGATCGT
GAGCCTGGTGGATCGGATCCTGATCGAAGCCCTCAGCATCGGCGCCAGCGACATCCACGTCGAGCCCTACGACCAGACCC
TTGAGATCCGCTTCCGGATCGACGGCGTGTTGCAGAAGCTGTTTGAGGATCTGCCCCGAAGTCTGGTGCCTGCCGTCACC
TCCAGGCTGAAGATCATGGCGGAACTGGACATCGCCGAACGACGGCTGCCTCAGGACGGCCGGATCCGACGCCTGTTCCG
GGGACGCAAGATGGACTTCCGCGTCAGCACCCTGCCGACACGCAATGGCGAAAAGGTGGTGCTGCGGCTGCTGGACAGCA
GCTCGACTGAGCTCGGCCTGGACACGCTGATCACCAATCCCGACGCCCGCGCAACCCTGCGGGAGCTCGGCAGCAATCCT
TACGGCATGGTCCTGGTCACGGGCCCCACGGGCTCCGGCAAATCCACCACGCTGTATGCGCTGCTGTCAGAGCGCAACAA
TCCAGACATCAACATCTCCACGGTGGAGGATCCCATTGAATACACCTTGCAGGGCATCACCCAGACCCAGGTGAACCGTG
ACAAGGGGCTGGACTTCCCCACCACCCTGCGGGCCTTCATGCGCCAGGACCCCGATGTGCTGCTGGTGGGAGAAACCCGC
GATCTGGAAACGGCCAAAACAGCCATCGAGGCCGCCCTCACCGGGCACCTGGTGTTCACCACCCTGCACTGCAACGATGC
GGCCAGTGCCATTACACGGCTGGATGAGATGGGGGTCGAACCCTTCATGGTGAGCGCTTCTCTGCTGGGCATCGTGTCGC
AGCGGCTGGTGCGCCGGGTCTGCAGCCAGTGCCGCATCAGCTACCACCCCGACCCAGAAGAACTGAGTCGATTCGGACTG
TTGGTAAGCGAAGTTTCCGATGTGACCTTCTATCGGGCCGACACGGGCCATGGGCGCCAGAAACGTGCCTGCCCCAACTG
CAATGGCAATGGCTACAAGGGGAGGGTGGCGGCGTTTGAAGTGCTGCGCATCAACGATGCGATCTCCACCGCCATCGCCC
AGCGCCAAACCACCGATGTGATCCGGCGCCTGGCCCTGGAAAGCGGTATGAAAACCCTGCTGGGCTATGGCCTTGAGCTG
GTGCGGGAAGGCCACACCACCCTGGCTGAAGTGCAGCGGATTCTGCTCACGGATACAGGGCTCGAGAGTGAGCGTCGCTC
CCGTGCCTTGAGCACCCTGACCTGCAAGGACTGCGGAGCCGGTCTCAGTGATGCTTGGCTGGAGTGTCCCTACTGCCTCA
CGCCCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8EIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.124

91.883

0.506

Multiple sequence alignment