Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PPS_RS25475 Genome accession   NC_015733
Coordinates   5611784..5612782 (-) Length   332 a.a.
NCBI ID   WP_013974616.1    Uniprot ID   -
Organism   Pseudomonas putida S16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5606784..5617782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPS_RS25455 (PPS_4934) argS 5607962..5609698 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  PPS_RS25460 (PPS_4935) - 5609700..5610398 (+) 699 WP_003257831.1 SPOR domain-containing protein -
  PPS_RS25465 (PPS_4936) - 5610435..5610854 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  PPS_RS25470 (PPS_4937) - 5611021..5611656 (+) 636 WP_013974615.1 C40 family peptidase -
  PPS_RS25475 (PPS_4938) pilT 5611784..5612782 (-) 999 WP_013974616.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PPS_RS25480 (PPS_4939) - 5612848..5613534 (+) 687 WP_013974617.1 YggS family pyridoxal phosphate-dependent enzyme -
  PPS_RS25485 (PPS_4940) proC 5613573..5614391 (+) 819 WP_013974618.1 pyrroline-5-carboxylate reductase -
  PPS_RS25490 (PPS_4941) - 5614402..5614992 (+) 591 WP_013974619.1 YggT family protein -
  PPS_RS25495 (PPS_4942) - 5615092..5616231 (+) 1140 WP_013974620.1 homoserine O-acetyltransferase -
  PPS_RS25500 (PPS_4943) metW 5616239..5616859 (+) 621 WP_013974621.1 methionine biosynthesis protein MetW -
  PPS_RS25505 (PPS_4944) - 5616882..5617316 (+) 435 WP_013974622.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35855.53 Da        Isoelectric Point: 6.3081

>NTDB_id=41680 PPS_RS25475 WP_013974616.1 5611784..5612782(-) (pilT) [Pseudomonas putida S16]
MDVTDLLARAVDAGASDLHLAAGQLPMLRLDGELQRMALPTLGPVALIEGMAPMLDEDQRRQWAQGDELDLALELPALGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCRDGLILVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=41680 PPS_RS25475 WP_013974616.1 5611784..5612782(-) (pilT) [Pseudomonas putida S16]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGTTACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGTCCGGTCGCCTTGATCGAGGGCATGGCCCCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGCGCTAGGGCGC
TTTCGGCTGAATCTGTTTCGCCAGTTGCACGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGCGATGTGTTTCAAGCTGTTGCGCAGTGCCGAGATGGCTTGATCCTCGTCGGTGGGCCGACCGGCA
GCGGCAAGTCCAGCACCTTGGCGACACTGCTTGACCAACTGAACCGCGATCGGGCTCTGCATATCATCACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGCCAAGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGTGCGGCTGAGACCGGGCACCTGGTACTGGCGACCGTGCATACCCGTTCGGCGGTGAGCAGTATTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTAGCGCAGGT
GCTGGTCAGGCGCGCGGGCGGAGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGCCAGCGGTGCGTAACCTGGTTC
GGGAAGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.424

99.398

0.521
  pilT Acinetobacter nosocomialis M2

51.515

99.398

0.512
  pilT Acinetobacter baumannii D1279779

51.515

99.398

0.512
  pilT Acinetobacter baumannii strain A118

51.515

99.398

0.512
  pilT Pseudomonas stutzeri DSM 10701

51.212

99.398

0.509
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

99.096

0.491
  pilT Vibrio cholerae strain A1552

49.544

99.096

0.491
  pilT Legionella pneumophila strain Lp02

49.091

99.398

0.488
  pilT Legionella pneumophila strain ERS1305867

49.091

99.398

0.488
  pilT Neisseria meningitidis 8013

48.338

99.699

0.482
  pilT Neisseria gonorrhoeae MS11

48.036

99.699

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.224

99.699

0.461
  pilU Pseudomonas stutzeri DSM 10701

37.16

99.699

0.37
  pilU Vibrio cholerae strain A1552

36.81

98.193

0.361

Multiple sequence alignment