Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GVI62_RS18230 Genome accession   NZ_CP047765
Coordinates   3795830..3796810 (-) Length   326 a.a.
NCBI ID   WP_060580281.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain Eho-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3790830..3801810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI62_RS18200 (GVI62_18165) - 3791711..3792208 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  GVI62_RS18205 (GVI62_18170) endA 3792303..3793010 (+) 708 WP_003860034.1 deoxyribonuclease I -
  GVI62_RS18210 (GVI62_18175) rsmE 3793062..3793793 (+) 732 WP_045345616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GVI62_RS18215 (GVI62_18180) gshB 3793821..3794768 (+) 948 WP_023323729.1 glutathione synthase -
  GVI62_RS18220 (GVI62_18185) - 3794843..3795403 (+) 561 WP_003860031.1 YqgE/AlgH family protein -
  GVI62_RS18225 (GVI62_18190) ruvX 3795403..3795819 (+) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  GVI62_RS18230 (GVI62_18195) pilT 3795830..3796810 (-) 981 WP_060580281.1 type IV pilus twitching motility protein PilT Machinery gene
  GVI62_RS18235 (GVI62_18200) - 3796828..3797529 (+) 702 WP_032610102.1 YggS family pyridoxal phosphate-dependent enzyme -
  GVI62_RS18240 (GVI62_18205) - 3797551..3798117 (+) 567 WP_003860024.1 YggT family protein -
  GVI62_RS18245 (GVI62_18210) yggU 3798114..3798410 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  GVI62_RS18250 (GVI62_18215) - 3798414..3799007 (+) 594 WP_022651831.1 XTP/dITP diphosphatase -
  GVI62_RS18255 (GVI62_18220) hemW 3799000..3800148 (+) 1149 WP_003860019.1 radical SAM family heme chaperone HemW -
  GVI62_RS18260 (GVI62_18225) - 3800205..3800567 (+) 363 WP_045337264.1 DUF559 domain-containing protein -
  GVI62_RS18265 (GVI62_18230) - 3800634..3801350 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  GVI62_RS18270 (GVI62_18235) - 3801408..3801734 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35670.69 Da        Isoelectric Point: 6.4125

>NTDB_id=415727 GVI62_RS18230 WP_060580281.1 3795830..3796810(-) (pilT) [Enterobacter hormaechei strain Eho-1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVYFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=415727 GVI62_RS18230 WP_060580281.1 3795830..3796810(-) (pilT) [Enterobacter hormaechei strain Eho-1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCTATTTTCTCAATCACCAGACGGACGGGCACATTCTGACCCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTAATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAACGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.75

98.16

0.38

Multiple sequence alignment