Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TOPB45_RS07780 Genome accession   NC_015682
Coordinates   1515558..1516649 (-) Length   363 a.a.
NCBI ID   WP_013910289.1    Uniprot ID   F8C3G2
Organism   Thermodesulfobacterium geofontis OPF15     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1510558..1521649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOPB45_RS07760 (TOPB45_1509) priA 1510692..1513046 (-) 2355 WP_013910285.1 primosomal protein N' -
  TOPB45_RS07765 (TOPB45_1510) - 1513046..1513927 (-) 882 WP_013910286.1 UTP--glucose-1-phosphate uridylyltransferase -
  TOPB45_RS07770 (TOPB45_1511) - 1513936..1514394 (-) 459 WP_013910287.1 hypothetical protein -
  TOPB45_RS07775 (TOPB45_1512) - 1514391..1515554 (-) 1164 WP_013910288.1 type IV pilus twitching motility protein PilT -
  TOPB45_RS07780 (TOPB45_1513) pilT 1515558..1516649 (-) 1092 WP_013910289.1 type IV pilus twitching motility protein PilT Machinery gene
  TOPB45_RS07785 (TOPB45_1514) - 1516703..1517137 (-) 435 WP_013910290.1 NIL domain-containing protein -
  TOPB45_RS07790 (TOPB45_1515) - 1517153..1518895 (-) 1743 WP_013910291.1 YcaO-like family protein -
  TOPB45_RS07795 (TOPB45_1516) mnmA 1518982..1520004 (+) 1023 WP_013910292.1 tRNA 2-thiouridine(34) synthase MnmA -
  TOPB45_RS07800 (TOPB45_1517) gatB 1520067..1521497 (+) 1431 WP_013910293.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40912.57 Da        Isoelectric Point: 6.3206

>NTDB_id=41442 TOPB45_RS07780 WP_013910289.1 1515558..1516649(-) (pilT) [Thermodesulfobacterium geofontis OPF15]
MAKLDPFFRLMVDTQASDLHLSAGNPPLLRVHGELQRVKYKVLEEDELREMLYEIASEEIIKKFEEEGEVDFGYEIPGLA
RFRINYFRQRKGIAAAFRLIPNKIKTVEELGLPPLLNKLALLPRGLVLVTGPTGAGKSTTLAAIIDYANRMRYDHIITIE
DPIEFVHEPKNCLINQREVGVHTKSFANALRAALREDPDIILVGEMRDLETIALAIEASLTGHLVFSTLHTIGAAQTVSR
IIDAFPPQERDQIRVSLSEALRAVVSQTMFKRIDKPGRIVAVEIMIATPAIRNLIRENKIHQIPSLIQTGKKYGMISLDD
CIMDYLNKGYISPEEAFIKAIDKSRFLPFIQDKEIADFTEIPG

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=41442 TOPB45_RS07780 WP_013910289.1 1515558..1516649(-) (pilT) [Thermodesulfobacterium geofontis OPF15]
ATGGCTAAACTTGATCCCTTTTTTAGATTAATGGTTGATACACAAGCTTCTGACCTTCATCTTTCTGCAGGGAATCCTCC
TCTTTTGAGGGTCCATGGTGAACTTCAAAGAGTTAAGTATAAAGTATTAGAGGAAGATGAATTAAGAGAAATGCTTTATG
AAATAGCTTCAGAAGAAATTATAAAAAAATTCGAAGAAGAAGGAGAAGTAGACTTTGGATATGAAATTCCTGGGCTTGCG
AGATTTAGAATTAACTATTTTCGTCAAAGAAAGGGTATTGCAGCAGCTTTTAGACTTATTCCAAATAAAATTAAAACCGT
AGAAGAATTAGGTCTTCCTCCACTTTTAAATAAACTTGCACTTTTACCAAGAGGACTAGTTTTAGTAACTGGTCCTACAG
GTGCTGGTAAGTCAACTACTCTTGCAGCGATTATAGATTACGCGAATAGAATGCGTTATGACCATATTATTACTATTGAA
GACCCTATTGAATTTGTACATGAACCTAAGAATTGTTTAATTAATCAAAGGGAAGTTGGAGTTCATACAAAAAGCTTTGC
CAATGCTTTAAGAGCAGCTTTAAGAGAAGATCCTGATATAATTCTTGTAGGTGAAATGCGTGATTTAGAAACTATAGCCC
TTGCTATTGAGGCTTCCCTTACTGGTCATTTAGTTTTTTCTACTTTACACACTATTGGTGCAGCTCAAACTGTAAGTAGA
ATAATAGATGCTTTTCCTCCCCAGGAAAGAGACCAAATAAGAGTATCTTTATCTGAAGCCTTAAGAGCAGTTGTTTCTCA
AACCATGTTTAAAAGAATTGATAAACCAGGAAGAATAGTTGCTGTAGAAATAATGATAGCCACCCCTGCCATTAGAAATC
TTATAAGAGAAAATAAAATTCATCAAATTCCATCTCTTATTCAAACAGGCAAAAAATATGGAATGATATCTTTAGATGAC
TGTATTATGGATTATTTAAATAAAGGATATATCTCTCCTGAAGAAGCTTTTATAAAAGCTATAGATAAAAGCAGGTTTTT
GCCTTTTATTCAAGATAAAGAAATTGCTGATTTTACTGAAATCCCAGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8C3G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

57.057

91.736

0.523
  pilT Acinetobacter nosocomialis M2

55.655

92.562

0.515
  pilT Acinetobacter baumannii D1279779

55.655

92.562

0.515
  pilT Acinetobacter baumannii strain A118

55.655

92.562

0.515
  pilT Legionella pneumophila strain ERS1305867

55.821

92.287

0.515
  pilT Legionella pneumophila strain Lp02

55.821

92.287

0.515
  pilT Pseudomonas aeruginosa PAK

57.538

89.532

0.515
  pilT Pseudomonas stutzeri DSM 10701

55.891

91.185

0.51
  pilT Neisseria meningitidis 8013

54.128

90.083

0.488
  pilT Neisseria gonorrhoeae MS11

53.988

89.807

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.16

89.256

0.466
  pilT Vibrio cholerae strain A1552

52.16

89.256

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.667

95.041

0.444
  pilU Pseudomonas stutzeri DSM 10701

39.941

93.113

0.372
  pilU Acinetobacter baylyi ADP1

39.053

93.113

0.364

Multiple sequence alignment