Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THEIN_RS10930 Genome accession   NC_015681
Coordinates   2221396..2222487 (-) Length   363 a.a.
NCBI ID   WP_013908730.1    Uniprot ID   F8ADH2
Organism   Thermodesulfatator indicus DSM 15286     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2216396..2227487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEIN_RS10915 (Thein_2140) galU 2218485..2219366 (-) 882 WP_013908727.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  THEIN_RS10920 (Thein_2141) - 2219625..2220230 (-) 606 WP_041434736.1 zinc ribbon domain-containing protein -
  THEIN_RS10925 (Thein_2142) - 2220224..2221399 (-) 1176 WP_013908729.1 PilT/PilU family type 4a pilus ATPase -
  THEIN_RS10930 (Thein_2143) pilT 2221396..2222487 (-) 1092 WP_013908730.1 type IV pilus twitching motility protein PilT Machinery gene
  THEIN_RS10935 (Thein_2144) ftsE 2222581..2223246 (+) 666 WP_013908731.1 cell division ATP-binding protein FtsE -
  THEIN_RS10940 (Thein_2145) - 2223243..2224118 (+) 876 WP_013908732.1 permease-like cell division protein FtsX -
  THEIN_RS10945 (Thein_2146) - 2224070..2225272 (+) 1203 WP_013908733.1 peptidoglycan DD-metalloendopeptidase family protein -
  THEIN_RS10950 (Thein_2147) - 2225280..2226638 (+) 1359 WP_013908734.1 S41 family peptidase -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40282.81 Da        Isoelectric Point: 6.9250

>NTDB_id=41434 THEIN_RS10930 WP_013908730.1 2221396..2222487(-) (pilT) [Thermodesulfatator indicus DSM 15286]
MAKIDAFLKLMVESGASDLHMASGNPPLMRIHGDLQRVKYKVLENDELKAMLYEITPEDRIKVFEETGDIDFGFELPGLA
RFRVNMFQQKNGVGAVFRLIPSQVKTAEELGLPPILTKLAMLPKGLVLVTGPTGSGKSTTLAAIIDYANKNRQDHILTIE
DPIEFVHKSYGCLVNQREVGVHTKSFAAALRSALREDPDIIMVGEMRDLETISLAIEAAMTGHLVLATLHTLNAPKTVDR
IIEIFPHEQQPQVRSTLADALRAVVSQTMFKRIDKPGRVVAFEILIATPAARNLIREGKTYQLPSVIQTGKRFGMRSLDD
SIMELLQKGYISPEEALNKAVDKARFLPLVNNKELSDFTEVPS

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=41434 THEIN_RS10930 WP_013908730.1 2221396..2222487(-) (pilT) [Thermodesulfatator indicus DSM 15286]
ATGGCCAAGATTGATGCCTTTTTAAAATTAATGGTAGAAAGTGGGGCCTCTGACCTGCACATGGCCTCGGGTAACCCCCC
GCTTATGCGGATTCACGGTGATCTTCAGCGGGTGAAATACAAGGTCCTTGAAAACGACGAGTTAAAGGCCATGCTTTACG
AAATTACCCCTGAAGATCGTATCAAGGTCTTTGAAGAAACTGGCGATATTGACTTTGGTTTTGAGCTACCAGGCCTGGCC
CGTTTTCGTGTAAATATGTTCCAGCAAAAAAACGGCGTGGGCGCGGTCTTCAGGCTTATTCCCTCTCAGGTAAAGACCGC
CGAAGAACTCGGGCTTCCGCCCATCCTTACCAAGCTCGCCATGCTGCCTAAAGGGCTTGTGCTGGTGACCGGGCCTACCG
GCTCAGGTAAATCAACAACTCTTGCGGCTATCATTGATTACGCCAACAAAAATCGTCAGGACCACATTTTAACCATAGAA
GACCCGATAGAATTTGTTCACAAGTCCTATGGCTGTCTGGTAAACCAGCGAGAGGTAGGAGTGCATACCAAAAGCTTTGC
CGCGGCCTTGCGCTCTGCCTTACGTGAAGACCCGGACATCATCATGGTGGGTGAAATGCGAGACCTTGAGACTATTTCTC
TCGCCATTGAAGCCGCTATGACCGGTCATTTGGTGCTGGCCACCCTTCATACCTTGAACGCTCCCAAAACCGTTGACCGT
ATCATTGAAATCTTTCCCCATGAACAACAACCCCAAGTTCGCTCAACTCTGGCCGATGCTTTACGAGCCGTGGTTTCACA
AACCATGTTTAAGCGCATAGATAAGCCCGGGCGTGTGGTGGCCTTTGAGATCCTTATAGCCACGCCAGCGGCTAGAAACT
TGATTCGCGAAGGCAAGACCTACCAGCTGCCTTCGGTTATCCAGACCGGAAAGAGGTTCGGCATGAGAAGCCTTGATGAT
TCTATCATGGAGTTGTTGCAAAAGGGTTATATTTCACCTGAGGAGGCCCTTAACAAGGCGGTGGATAAAGCCCGCTTCCT
GCCTTTGGTGAATAATAAAGAACTCAGCGATTTTACCGAGGTGCCCTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8ADH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

56.677

92.837

0.526
  pilT Pseudomonas stutzeri DSM 10701

55.685

94.49

0.526
  pilT Acinetobacter baumannii strain A118

54.651

94.766

0.518
  pilT Acinetobacter baumannii D1279779

54.651

94.766

0.518
  pilT Acinetobacter nosocomialis M2

54.651

94.766

0.518
  pilT Legionella pneumophila strain Lp02

54.81

94.49

0.518
  pilT Legionella pneumophila strain ERS1305867

54.81

94.49

0.518
  pilT Acinetobacter baylyi ADP1

56.535

90.634

0.512
  pilT Neisseria meningitidis 8013

53.55

93.113

0.499
  pilT Neisseria gonorrhoeae MS11

53.254

93.113

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

96.143

0.479
  pilT Vibrio cholerae strain A1552

51.19

92.562

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.19

92.562

0.474
  pilU Pseudomonas stutzeri DSM 10701

42.09

92.287

0.388
  pilU Vibrio cholerae strain A1552

39.655

95.868

0.38

Multiple sequence alignment