Detailed information NTG0409147

Bioinformatically predicted

Overview

• Name: comGC/cglC
• Type: Machinery gene
• Locus tag: GP482_RS08810
• Genome accession: NZ_CP046875
• Coordinates: 1769017..1769310 (+)
• Length: 97 a.a.
• NCBI ID: WP_074561132.1
• Uniprot ID: A0A6G8I001
• Organism: Streptococcus ruminicola strain CNU_77-61
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1764017..1774310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GP482_RS08795 (GP482_08795)	-	1766668..1767036 (+)	369	WP_074564836.1	DUF1033 family protein	-
GP482_RS08800 (GP482_08800)	comYA	1767109..1768050 (+)	942	WP_074603320.1	competence type IV pilus ATPase ComGA	Machinery gene
GP482_RS08805 (GP482_08805)	comYB	1767974..1769017 (+)	1044	WP_174235044.1	competence type IV pilus assembly protein ComGB	Machinery gene
GP482_RS08810 (GP482_08810)	comGC/cglC	1769017..1769310 (+)	294	WP_074561132.1	competence type IV pilus major pilin ComGC	Machinery gene
GP482_RS08815 (GP482_08815)	comYD	1769294..1769725 (+)	432	WP_158914695.1	competence type IV pilus minor pilin ComGD	Machinery gene
GP482_RS08820 (GP482_08820)	comYE	1769679..1769972 (+)	294	WP_158914697.1	competence type IV pilus minor pilin ComGE	Machinery gene
GP482_RS08825 (GP482_08825)	comYF	1769956..1770393 (+)	438	WP_158914699.1	competence type IV pilus minor pilin ComGF	Machinery gene
GP482_RS08830 (GP482_08830)	comGG	1770365..1770715 (+)	351	WP_158914701.1	competence type IV pilus minor pilin ComGG	-
GP482_RS08835 (GP482_08835)	comYH	1770769..1771725 (+)	957	WP_158914703.1	class I SAM-dependent methyltransferase	Machinery gene
GP482_RS08840 (GP482_08840)	-	1771779..1772978 (+)	1200	WP_158914705.1	acetate kinase	-
GP482_RS08845 (GP482_08845)	-	1773137..1773334 (+)	198	WP_074561137.1	helix-turn-helix transcriptional regulator	-
GP482_RS08850 (GP482_08850)	-	1773350..1773550 (+)	201	WP_074965270.1	hypothetical protein	-
GP482_RS08855 (GP482_08855)	-	1773551..1774006 (+)	456	WP_234787223.1	ABC transporter permease	-

Sequence

Protein

• Length: 97 a.a.
• Molecular weight: 10995.12 Da
• Isoelectric Point: 9.6932

>NTDB_id=409147 GP482_RS08810 WP_074561132.1 1769017..1769310(+) (comGC/cglC) [Streptococcus ruminicola strain CNU_77-61]
MKKIWKKLRKKSVEAFTLVEMLIVLLIIGVLMLLFVPNLSKQKDVVHEKGDAAVVKVVESQMDLYEVKTGDKASVDDLVD
IGYITKEQAKTYNEAKK

Nucleotide

• Length: 294 bp

>NTDB_id=409147 GP482_RS08810 WP_074561132.1 1769017..1769310(+) (comGC/cglC) [Streptococcus ruminicola strain CNU_77-61]
ATGAAAAAAATCTGGAAAAAACTACGTAAAAAGTCTGTGGAAGCTTTCACACTTGTGGAAATGCTGATTGTTTTGCTAAT
CATTGGTGTCTTGATGCTATTATTTGTGCCAAACTTGAGTAAGCAAAAGGATGTTGTGCATGAAAAAGGAGATGCTGCTG
TTGTCAAAGTTGTCGAAAGTCAGATGGACCTTTATGAGGTGAAGACAGGCGATAAGGCAAGTGTTGATGATTTAGTAGAC
ATTGGCTACATTACGAAAGAGCAAGCCAAAACCTATAATGAAGCTAAAAAATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (13-34)

3D structure

• AlphaFold DB: A0A6G8I001

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGC/cglC	Streptococcus mitis SK321	62.766	96.907	0.608
comYC	Streptococcus suis isolate S10	64.13	94.845	0.608
comGC/cglC	Streptococcus pneumoniae Rx1	61.702	96.907	0.598
comGC/cglC	Streptococcus mitis NCTC 12261	61.702	96.907	0.598
comGC/cglC	Streptococcus pneumoniae R6	61.702	96.907	0.598
comGC/cglC	Streptococcus pneumoniae D39	61.702	96.907	0.598
comGC/cglC	Streptococcus pneumoniae TIGR4	61.702	96.907	0.598
comYC	Streptococcus gordonii str. Challis substr. CH1	65.517	89.691	0.588
comYC	Streptococcus mutans UA140	60.92	89.691	0.546
comYC	Streptococcus mutans UA159	60.92	89.691	0.546
comGC	Lactococcus lactis subsp. cremoris KW2	53.333	92.784	0.495
comGC	Staphylococcus aureus MW2	45.122	84.536	0.381
comGC	Staphylococcus aureus N315	45.122	84.536	0.381
comGC	Latilactobacillus sakei subsp. sakei 23K	43.023	88.66	0.381