Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   GP482_RS08800 Genome accession   NZ_CP046875
Coordinates   1767109..1768050 (+) Length   313 a.a.
NCBI ID   WP_074603320.1    Uniprot ID   -
Organism   Streptococcus ruminicola strain CNU_77-61     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1762109..1773050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP482_RS08790 (GP482_08790) rpoC 1762874..1766512 (+) 3639 WP_074603316.1 DNA-directed RNA polymerase subunit beta' -
  GP482_RS08795 (GP482_08795) - 1766668..1767036 (+) 369 WP_074564836.1 DUF1033 family protein -
  GP482_RS08800 (GP482_08800) comYA 1767109..1768050 (+) 942 WP_074603320.1 competence type IV pilus ATPase ComGA Machinery gene
  GP482_RS08805 (GP482_08805) comYB 1767974..1769017 (+) 1044 WP_174235044.1 competence type IV pilus assembly protein ComGB Machinery gene
  GP482_RS08810 (GP482_08810) comGC/cglC 1769017..1769310 (+) 294 WP_074561132.1 competence type IV pilus major pilin ComGC Machinery gene
  GP482_RS08815 (GP482_08815) comYD 1769294..1769725 (+) 432 WP_158914695.1 competence type IV pilus minor pilin ComGD Machinery gene
  GP482_RS08820 (GP482_08820) comYE 1769679..1769972 (+) 294 WP_158914697.1 competence type IV pilus minor pilin ComGE Machinery gene
  GP482_RS08825 (GP482_08825) comYF 1769956..1770393 (+) 438 WP_158914699.1 competence type IV pilus minor pilin ComGF Machinery gene
  GP482_RS08830 (GP482_08830) comGG 1770365..1770715 (+) 351 WP_158914701.1 competence type IV pilus minor pilin ComGG -
  GP482_RS08835 (GP482_08835) comYH 1770769..1771725 (+) 957 WP_158914703.1 class I SAM-dependent methyltransferase Machinery gene
  GP482_RS08840 (GP482_08840) - 1771779..1772978 (+) 1200 WP_158914705.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35658.77 Da        Isoelectric Point: 5.3172

>NTDB_id=409145 GP482_RS08800 WP_074603320.1 1767109..1768050(+) (comYA) [Streptococcus ruminicola strain CNU_77-61]
MIQEKAKQLIKDAVEKNAQDIYIVPKKEHYEIYQRVGDERQFIQELASDEMTSVISHFKFVAGMNVGEKRRSQLGSCDYE
FAEGEEISLRLSTVGDYRGRESLVIRLLYSGRHDLQYWFDGMKNILEAIDSRGLYLFSGPVGSGKTTLMYQLVREKFPDK
QVITIEDPVEIKQDNMLQLQLNDEIGMTYDNLIKLSLRHRPDILIIGEIRDSETARAVIRASLTGVLVFSTIHAKSIPGV
YARLLELGVSKQELENSLRAIIYQRLIGGGGVVDFAKGDFEQYPPTRWNEQIESLARDGHISLEEAKIEKIAL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=409145 GP482_RS08800 WP_074603320.1 1767109..1768050(+) (comYA) [Streptococcus ruminicola strain CNU_77-61]
ATGATTCAGGAAAAAGCAAAGCAGCTGATCAAGGATGCTGTTGAAAAGAATGCGCAAGATATTTACATTGTTCCTAAGAA
GGAGCACTATGAAATTTATCAACGTGTTGGTGATGAACGTCAGTTTATTCAGGAGCTTGCTAGTGATGAGATGACAAGTG
TGATTAGTCACTTTAAATTTGTGGCTGGGATGAATGTTGGTGAGAAACGTCGTAGTCAGCTAGGCTCTTGTGATTATGAA
TTTGCTGAGGGTGAAGAGATTTCACTGCGTTTATCCACTGTTGGTGATTATCGAGGGCGTGAGAGCCTGGTCATCAGACT
TCTTTATTCAGGTCGTCATGATTTGCAATATTGGTTCGATGGGATGAAGAATATTTTAGAGGCGATTGATAGTCGAGGTC
TTTATCTTTTTTCCGGGCCGGTAGGGAGTGGAAAGACTACTCTCATGTATCAATTGGTTCGTGAAAAGTTCCCTGATAAG
CAGGTCATTACGATAGAAGATCCTGTTGAGATTAAGCAGGACAATATGTTGCAGCTGCAGCTTAATGATGAGATTGGCAT
GACTTATGATAATCTTATCAAGCTCTCGCTTCGTCATAGACCTGATATTTTGATTATTGGTGAGATTCGTGATAGTGAGA
CAGCGCGTGCGGTTATTAGAGCTAGTCTGACAGGTGTGCTCGTCTTTTCAACCATTCATGCTAAGAGTATCCCGGGTGTC
TATGCAAGATTATTAGAATTGGGAGTTAGTAAGCAGGAACTTGAAAATAGTTTGCGAGCTATTATTTATCAGCGATTAAT
TGGCGGTGGAGGTGTTGTGGATTTTGCCAAAGGAGATTTTGAACAGTATCCCCCAACAAGGTGGAATGAGCAAATTGAAA
GCCTTGCTAGGGACGGACATATTAGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

71.061

99.361

0.706
  comYA Streptococcus mutans UA140

70.74

99.361

0.703
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.419

99.042

0.668
  comYA Streptococcus gordonii str. Challis substr. CH1

65.595

99.361

0.652
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.064

99.681

0.649
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.103

99.681

0.639
  comGA Lactococcus lactis subsp. cremoris KW2

54.633

100

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

43.431

87.54

0.38

Multiple sequence alignment