Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GP482_RS08805 Genome accession   NZ_CP046875
Coordinates   1767974..1769017 (+) Length   347 a.a.
NCBI ID   WP_174235044.1    Uniprot ID   -
Organism   Streptococcus ruminicola strain CNU_77-61     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1762974..1774017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP482_RS08795 (GP482_08795) - 1766668..1767036 (+) 369 WP_074564836.1 DUF1033 family protein -
  GP482_RS08800 (GP482_08800) comYA 1767109..1768050 (+) 942 WP_074603320.1 competence type IV pilus ATPase ComGA Machinery gene
  GP482_RS08805 (GP482_08805) comYB 1767974..1769017 (+) 1044 WP_174235044.1 competence type IV pilus assembly protein ComGB Machinery gene
  GP482_RS08810 (GP482_08810) comGC/cglC 1769017..1769310 (+) 294 WP_074561132.1 competence type IV pilus major pilin ComGC Machinery gene
  GP482_RS08815 (GP482_08815) comYD 1769294..1769725 (+) 432 WP_158914695.1 competence type IV pilus minor pilin ComGD Machinery gene
  GP482_RS08820 (GP482_08820) comYE 1769679..1769972 (+) 294 WP_158914697.1 competence type IV pilus minor pilin ComGE Machinery gene
  GP482_RS08825 (GP482_08825) comYF 1769956..1770393 (+) 438 WP_158914699.1 competence type IV pilus minor pilin ComGF Machinery gene
  GP482_RS08830 (GP482_08830) comGG 1770365..1770715 (+) 351 WP_158914701.1 competence type IV pilus minor pilin ComGG -
  GP482_RS08835 (GP482_08835) comYH 1770769..1771725 (+) 957 WP_158914703.1 class I SAM-dependent methyltransferase Machinery gene
  GP482_RS08840 (GP482_08840) - 1771779..1772978 (+) 1200 WP_158914705.1 acetate kinase -
  GP482_RS08845 (GP482_08845) - 1773137..1773334 (+) 198 WP_074561137.1 helix-turn-helix transcriptional regulator -
  GP482_RS08850 (GP482_08850) - 1773350..1773550 (+) 201 WP_074965270.1 hypothetical protein -
  GP482_RS08855 (GP482_08855) - 1773551..1774006 (+) 456 WP_234787223.1 ABC transporter permease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39588.98 Da        Isoelectric Point: 9.6038

>NTDB_id=409146 GP482_RS08805 WP_174235044.1 1767974..1769017(+) (comYB) [Streptococcus ruminicola strain CNU_77-61]
MSKLKALLGTDILALRKQKSKKLPFKKQQKVIQLFNNLFNSGFNLTEIVSFLRRSQLLAEVYVDSMQESLLSGASLADMM
AKLGFSDTIVTQIALADVHGNCQQSLLKIDGYLASMSVVRKKLIEVATYPLILLSFLILIMLGLKNYLLPQLENQNFATQ
IISHFPMIFLGGFALLALGILLGVLYAKRLSPIYLYSQLSRLPIFGRYVRLYLTAYYAREWGNLIGQGIELMEIVELMQR
QKSRLFQEIGKDMQEALLSGQSFHQKVLDYPFFLRELSLMIEYGEVKSKLGRELDIYAEETWQNFFSQLTQATQLIQPLV
FVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=409146 GP482_RS08805 WP_174235044.1 1767974..1769017(+) (comYB) [Streptococcus ruminicola strain CNU_77-61]
ATGAGCAAATTGAAAGCCTTGCTAGGGACGGACATATTAGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAAAAA
ACAGCAGAAGGTGATTCAATTATTTAACAATCTCTTTAACAGTGGCTTTAATTTAACAGAAATTGTTTCTTTTTTGAGAC
GAAGTCAATTGTTGGCAGAAGTTTATGTGGATAGTATGCAAGAATCTTTGCTAAGCGGGGCAAGTTTGGCAGATATGATG
GCTAAATTAGGCTTTTCAGATACAATTGTCACTCAGATTGCTTTGGCAGATGTTCATGGAAATTGTCAGCAGAGTTTGTT
AAAAATTGATGGCTACTTGGCCAGTATGTCAGTTGTTCGAAAGAAATTGATTGAAGTGGCGACTTATCCTTTGATTTTGC
TCAGCTTTTTAATTCTGATTATGCTGGGCTTGAAAAATTATTTGCTACCGCAGTTGGAAAATCAAAATTTTGCGACTCAG
ATTATCAGCCATTTTCCTATGATTTTTTTAGGTGGCTTTGCTCTTTTAGCTTTAGGAATTCTGCTTGGGGTTCTGTATGC
CAAGCGCTTATCGCCAATTTATCTGTATAGTCAGTTGAGTCGTTTGCCAATCTTTGGGCGGTACGTGCGTCTTTATTTGA
CCGCCTATTATGCGCGTGAGTGGGGAAATCTCATCGGGCAAGGTATTGAGCTTATGGAGATTGTAGAGCTCATGCAGCGG
CAAAAATCACGCCTTTTTCAGGAAATTGGAAAAGACATGCAGGAAGCTTTATTGTCAGGTCAATCTTTTCATCAGAAAGT
GCTGGATTACCCTTTCTTTTTGCGTGAACTTAGCTTAATGATTGAATACGGCGAGGTTAAGTCAAAATTAGGTCGTGAGT
TAGATATTTATGCCGAAGAGACTTGGCAGAATTTCTTTAGTCAATTGACACAGGCAACGCAGCTGATTCAGCCTTTGGTC
TTTGTCTTTGTTGCTTTAATCATCGTTTTAATTTATGTGGCAATGCTTTTGCCAATGTATCAAAATATGGGAGGAAATTT
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

65.506

91.066

0.597
  comYB Streptococcus mutans UA159

65.506

91.066

0.597
  comYB Streptococcus gordonii str. Challis substr. CH1

58.529

97.983

0.573
  comGB/cglB Streptococcus mitis NCTC 12261

58.457

97.118

0.568
  comGB/cglB Streptococcus mitis SK321

57.567

97.118

0.559
  comGB/cglB Streptococcus pneumoniae Rx1

56.973

97.118

0.553
  comGB/cglB Streptococcus pneumoniae D39

56.973

97.118

0.553
  comGB/cglB Streptococcus pneumoniae R6

56.973

97.118

0.553
  comGB/cglB Streptococcus pneumoniae TIGR4

56.973

97.118

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

51.786

96.83

0.501

Multiple sequence alignment