Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GO174_RS10660 Genome accession   NZ_CP046857
Coordinates   2265195..2265617 (+) Length   140 a.a.
NCBI ID   WP_158125403.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0233     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2260195..2270617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO174_RS10635 dsbC 2261016..2261780 (-) 765 WP_158125400.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GO174_RS10640 xerD 2261797..2262651 (-) 855 WP_230857653.1 site-specific tyrosine recombinase XerD -
  GO174_RS10645 fldB 2262846..2263367 (+) 522 WP_020430452.1 flavodoxin FldB -
  GO174_RS10650 ampD 2263372..2263923 (-) 552 WP_158125401.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GO174_RS10655 nadC 2264076..2264963 (+) 888 WP_158125402.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GO174_RS10660 pilA 2265195..2265617 (+) 423 WP_158125403.1 pilin Machinery gene
  GO174_RS10665 pilB 2265624..2267312 (+) 1689 WP_158125404.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GO174_RS10670 pilC 2267348..2268574 (+) 1227 WP_158125405.1 type II secretion system F family protein Machinery gene
  GO174_RS10675 pilD 2268626..2269495 (+) 870 WP_047459100.1 A24 family peptidase Machinery gene
  GO174_RS10680 coaE 2269498..2270106 (+) 609 WP_158125406.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14585.77 Da        Isoelectric Point: 9.4843

>NTDB_id=408998 GO174_RS10660 WP_158125403.1 2265195..2265617(+) (pilA) [Vibrio fluvialis strain 2015AW-0233]
MNANQRKQQQGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEASAALASITGQRVNVESYVITEGVFPTTTQVPVPSSA
LGTITYANAASAAGDIIFTFKSSGVSPDVVSKKVELKRTSAGAWSCSTTVATDLKPKGCS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=408998 GO174_RS10660 WP_158125403.1 2265195..2265617(+) (pilA) [Vibrio fluvialis strain 2015AW-0233]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTAGCAATTATTGGGGTGTT
GGCAGCAATCGCTGTTCCTCAGTATCAAAAGTATGTTGCAAAAAGTGAGGCTTCAGCAGCACTAGCATCTATAACAGGAC
AGCGAGTTAATGTTGAGTCTTATGTAATAACAGAAGGCGTATTTCCTACAACAACCCAGGTACCCGTACCTTCCTCTGCA
TTGGGAACAATAACCTATGCTAATGCAGCATCTGCTGCTGGGGATATCATATTTACATTCAAATCTTCAGGTGTAAGCCC
AGATGTTGTCAGTAAAAAAGTGGAACTAAAGAGAACAAGTGCTGGTGCATGGAGCTGTAGTACAACTGTAGCGACAGACT
TGAAGCCTAAAGGCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.669

100

0.536
  pilA Vibrio cholerae strain A1552

49.669

100

0.536
  pilA Vibrio cholerae C6706

49.669

100

0.536
  pilA Pseudomonas aeruginosa PAK

43.919

100

0.464
  pilA Acinetobacter baumannii strain A118

43.151

100

0.45
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.357

100

0.443
  pilA Vibrio parahaemolyticus RIMD 2210633

39.041

100

0.407
  comP Acinetobacter baylyi ADP1

34.81

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

35.333

100

0.379
  pilA2 Legionella pneumophila str. Paris

34.667

100

0.371
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.806

95.714

0.371
  pilA Acinetobacter nosocomialis M2

40.8

89.286

0.364

Multiple sequence alignment