Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GO174_RS10665 Genome accession   NZ_CP046857
Coordinates   2265624..2267312 (+) Length   562 a.a.
NCBI ID   WP_158125404.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0233     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2260624..2272312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO174_RS10635 dsbC 2261016..2261780 (-) 765 WP_158125400.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GO174_RS10640 xerD 2261797..2262651 (-) 855 WP_230857653.1 site-specific tyrosine recombinase XerD -
  GO174_RS10645 fldB 2262846..2263367 (+) 522 WP_020430452.1 flavodoxin FldB -
  GO174_RS10650 ampD 2263372..2263923 (-) 552 WP_158125401.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GO174_RS10655 nadC 2264076..2264963 (+) 888 WP_158125402.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GO174_RS10660 pilA 2265195..2265617 (+) 423 WP_158125403.1 pilin Machinery gene
  GO174_RS10665 pilB 2265624..2267312 (+) 1689 WP_158125404.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GO174_RS10670 pilC 2267348..2268574 (+) 1227 WP_158125405.1 type II secretion system F family protein Machinery gene
  GO174_RS10675 pilD 2268626..2269495 (+) 870 WP_047459100.1 A24 family peptidase Machinery gene
  GO174_RS10680 coaE 2269498..2270106 (+) 609 WP_158125406.1 dephospho-CoA kinase -
  GO174_RS10685 zapD 2270139..2270879 (+) 741 WP_020332196.1 cell division protein ZapD -
  GO174_RS10690 yacG 2270934..2271131 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62054.90 Da        Isoelectric Point: 6.2647

>NTDB_id=408999 GO174_RS10665 WP_158125404.1 2265624..2267312(+) (pilB) [Vibrio fluvialis strain 2015AW-0233]
MNTNLLAILRQADLLSPTQEQAVMEHIHASGTNVPEALIDLAIFHHHELTEHLSQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQVLTLAVSDPTHLQVEDEFRFATGLQVELVLADHKALQAAIRRLYGRAITSHSTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=408999 GO174_RS10665 WP_158125404.1 2265624..2267312(+) (pilB) [Vibrio fluvialis strain 2015AW-0233]
ATGAACACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTGATGGAGCATAT
TCACGCTTCCGGAACCAATGTTCCGGAAGCGTTGATCGATTTGGCGATCTTCCATCACCATGAACTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCGTTTTGACTTCGCGCCACTGTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAATAGCAACGGTCAGGTACTGACTCTTGCGGTATCCGATCCGACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAGTAGAACTGGTATTAGCTGATCATAAAGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCACGTCACATAGCACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTTATTAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCCAGCCATTTAAGCCGTCGACTGGCCGCGCGGATTAAGATT
TTGTCGAAGCTCGATATCGCCGAGCGACGTTTACCGCAAGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTTTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCGTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACCCTGCACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTTAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GACGCCTTTGCCCTCACTGCAAAGTCGCCACATCACCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAATGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.74

100

0.827
  pilB Vibrio campbellii strain DS40M4

72.776

100

0.728
  pilB Vibrio parahaemolyticus RIMD 2210633

72.064

100

0.721
  pilB Legionella pneumophila strain ERS1305867

52.611

91.993

0.484
  pilB Acinetobacter baumannii D1279779

50.096

92.349

0.463
  pilB Acinetobacter baylyi ADP1

48.767

93.772

0.457
  pilF Neisseria gonorrhoeae MS11

45.15

100

0.456
  pilF Thermus thermophilus HB27

37.057

100

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368

Multiple sequence alignment