Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GMX10_RS10915 Genome accession   NZ_CP046377
Coordinates   2377850..2378866 (+) Length   338 a.a.
NCBI ID   WP_039486299.1    Uniprot ID   A0AAP9IGW8
Organism   Pectobacterium parvum strain PZ1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2372850..2383866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMX10_RS10885 (GMX10_10905) hemW 2373574..2374710 (-) 1137 WP_039486306.1 radical SAM family heme chaperone HemW -
  GMX10_RS10890 (GMX10_10910) rdgB 2374703..2375296 (-) 594 WP_039486304.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GMX10_RS10895 (GMX10_10915) yggU 2375328..2375618 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  GMX10_RS10900 (GMX10_10920) - 2375615..2376169 (-) 555 WP_039486302.1 YggT family protein -
  GMX10_RS10905 (GMX10_10925) proC 2376219..2377040 (-) 822 WP_161547152.1 pyrroline-5-carboxylate reductase -
  GMX10_RS10910 (GMX10_10930) - 2377116..2377829 (-) 714 WP_039486301.1 YggS family pyridoxal phosphate-dependent enzyme -
  GMX10_RS10915 (GMX10_10935) pilT 2377850..2378866 (+) 1017 WP_039486299.1 type IV pilus twitching motility protein PilT Machinery gene
  GMX10_RS10920 (GMX10_10940) - 2378835..2380667 (-) 1833 WP_039486297.1 diguanylate cyclase -
  GMX10_RS10925 (GMX10_10945) - 2381320..2382891 (-) 1572 WP_039486296.1 methyl-accepting chemotaxis protein -
  GMX10_RS10930 (GMX10_10950) nsrR 2383218..2383643 (+) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36649.80 Da        Isoelectric Point: 6.4958

>NTDB_id=403622 GMX10_RS10915 WP_039486299.1 2377850..2378866(+) (pilT) [Pectobacterium parvum strain PZ1]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLEALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LVDVFPGEEKAYVRSQLATCLQAAVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLTSHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=403622 GMX10_RS10915 WP_039486299.1 2377850..2378866(+) (pilT) [Pectobacterium parvum strain PZ1]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCAGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGCGCGCTAATGCTGCCCGATGGGCAA
CGCCTGCGGGTGAATGTGTTTCGTCAGCGGGAAGGGTTGTCTGCGGCGCTGCGTATTATCCCGTCCGCTCAGCCTTCATT
GGAAGCGCTACAGGCGCCACCCGTTCTATCGACGCTGTTGGAGAAACCGAACGGATTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCATTAAACGATAGCAGCGATCGCCATGTGATAACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGCGCGTCGTTTGC
ACAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGTCATCTGGTGTTGTCGACGCTGCATACACGTAGCGCGTCGCAGGCAGTTGATCGT
TTGGTTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCCGCCGTGACGCA
AAAATTATTACCCGCCGCACAAGGTGGGCGAATCGCACTGTATGAAGTGCTGACTGCGACTGCGGCAGTCAGTAACCTCA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAACGCTGTCGGGATGGGCTTACTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50.909

97.633

0.497
  pilT Neisseria gonorrhoeae MS11

50.606

97.633

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.338

97.929

0.473
  pilT Legionella pneumophila strain ERS1305867

48.338

97.929

0.473
  pilT Acinetobacter baylyi ADP1

48.338

97.929

0.473
  pilT Acinetobacter baumannii D1279779

48.036

97.929

0.47
  pilT Acinetobacter nosocomialis M2

48.036

97.929

0.47
  pilT Acinetobacter baumannii strain A118

48.036

97.929

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.26

94.379

0.408
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

41.121

94.97

0.391

Multiple sequence alignment