Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAHAQ_RS17600 Genome accession   NC_015061
Coordinates   3834274..3835296 (-) Length   340 a.a.
NCBI ID   WP_013576829.1    Uniprot ID   A0A0H3FEB0
Organism   Rahnella aceris     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3829274..3840296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAHAQ_RS17570 (Rahaq_3539) - 3830054..3830575 (+) 522 WP_041673163.1 SprT family zinc-dependent metalloprotease -
  RAHAQ_RS17575 (Rahaq_3540) endA 3830669..3831382 (+) 714 WP_013576824.1 deoxyribonuclease I -
  RAHAQ_RS17580 (Rahaq_3541) rsmE 3831470..3832201 (+) 732 WP_013576825.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RAHAQ_RS17585 (Rahaq_3542) gshB 3832213..3833163 (+) 951 WP_013576826.1 glutathione synthase -
  RAHAQ_RS17590 (Rahaq_3543) - 3833292..3833855 (+) 564 WP_013576827.1 YqgE/AlgH family protein -
  RAHAQ_RS17595 (Rahaq_3544) ruvX 3833855..3834283 (+) 429 WP_013576828.1 Holliday junction resolvase RuvX -
  RAHAQ_RS17600 (Rahaq_3545) pilT 3834274..3835296 (-) 1023 WP_013576829.1 type IV pilus twitching motility protein PilT Machinery gene
  RAHAQ_RS17605 (Rahaq_3546) - 3835319..3836038 (+) 720 WP_013576830.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAHAQ_RS17610 (Rahaq_3547) proC 3836111..3836932 (+) 822 WP_013576831.1 pyrroline-5-carboxylate reductase -
  RAHAQ_RS17615 (Rahaq_3548) - 3837019..3837573 (+) 555 WP_013576832.1 YggT family protein -
  RAHAQ_RS17620 (Rahaq_3549) yggU 3837558..3837863 (+) 306 WP_013576833.1 DUF167 family protein YggU -
  RAHAQ_RS17625 (Rahaq_3550) - 3837885..3838493 (+) 609 WP_013576834.1 XTP/dITP diphosphatase -
  RAHAQ_RS17630 (Rahaq_3551) hemW 3838486..3839622 (+) 1137 WP_013576835.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37043.55 Da        Isoelectric Point: 6.6137

>NTDB_id=39832 RAHAQ_RS17600 WP_013576829.1 3834274..3835296(-) (pilT) [Rahnella aceris]
MDIDGLVGRSVKQNASDLHLCTGYPPVLRIDGELCRCEDLPVVEASQMLGLIHAWLDATEQHTLQLAGQVDKALTLGDGV
RMRLNIFQQLHGLSAALRPVPARCPELAALRVPDILQHVIMQEDGLILVTGATGSGKSTTLAALIDHLNRHAKRHIITLE
DPIELIHTGRHCLIQQRELGLHTRSFGEAVRGALREDPDVLLLGELRDTDSIRLALTAAETGHLVLATLHTRNATQAIDR
LVDVFPAEEKAFVRAQLAGSLRAVIAQKLAVTEEKDGRKAGRVALFEVLTSTPAVCNLIREGKTHQLADILQTGAQAGMQ
TFAQSRQQRQRAGLLAEDQD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=39832 RAHAQ_RS17600 WP_013576829.1 3834274..3835296(-) (pilT) [Rahnella aceris]
ATGGATATTGATGGATTAGTGGGACGTAGTGTAAAGCAAAATGCCTCCGATCTGCACCTTTGTACCGGCTATCCGCCTGT
GTTGCGCATTGACGGGGAATTATGTCGCTGTGAAGATCTGCCGGTGGTGGAGGCTTCGCAGATGCTGGGGCTGATCCATG
CGTGGCTGGATGCCACTGAACAACATACTTTGCAGCTGGCCGGACAGGTGGATAAAGCGCTGACATTGGGTGATGGCGTG
CGGATGCGGCTCAATATCTTTCAGCAACTGCACGGGTTGTCTGCGGCGCTGAGACCGGTGCCTGCCCGTTGTCCCGAGCT
GGCTGCGTTGAGGGTGCCGGATATCTTGCAGCATGTCATTATGCAGGAAGACGGCCTGATTTTGGTCACCGGTGCGACGG
GCAGCGGGAAATCCACCACGCTGGCGGCGCTGATTGATCATCTCAACCGCCATGCGAAGCGCCATATTATTACGCTGGAA
GACCCCATTGAGCTTATCCATACTGGCAGGCACTGTCTGATTCAGCAGCGCGAACTCGGGCTGCATACGCGTTCATTTGG
TGAAGCGGTGCGCGGTGCTCTGCGTGAAGATCCGGATGTATTGTTACTGGGCGAACTGCGTGACACTGACAGTATCCGGC
TGGCGCTGACGGCGGCAGAAACCGGCCATCTGGTTCTCGCGACACTGCATACGCGCAACGCCACACAGGCCATTGACCGG
CTGGTTGATGTGTTTCCGGCAGAAGAAAAAGCGTTTGTCCGCGCGCAACTGGCGGGTAGCCTGCGGGCCGTGATCGCGCA
AAAACTGGCGGTGACTGAAGAAAAGGACGGTAGAAAAGCGGGAAGGGTAGCGCTTTTTGAAGTGCTGACTTCCACACCGG
CGGTGTGCAATTTAATCCGTGAAGGGAAAACTCATCAGCTGGCGGATATCCTGCAAACCGGCGCGCAGGCCGGCATGCAG
ACGTTCGCACAAAGCCGTCAGCAGCGGCAACGTGCGGGATTACTGGCGGAAGATCAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3FEB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.305

96.471

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

96.471

0.485
  pilT Neisseria meningitidis 8013

47.041

99.412

0.468
  pilT Neisseria gonorrhoeae MS11

46.746

99.412

0.465
  pilT Acinetobacter baylyi ADP1

47.164

98.529

0.465
  pilT Pseudomonas stutzeri DSM 10701

45.723

99.706

0.456
  pilT Pseudomonas aeruginosa PAK

47.826

94.706

0.453
  pilT Acinetobacter baumannii D1279779

47.077

95.588

0.45
  pilT Acinetobacter baumannii strain A118

47.077

95.588

0.45
  pilT Acinetobacter nosocomialis M2

47.077

95.588

0.45
  pilT Legionella pneumophila strain ERS1305867

46.296

95.294

0.441
  pilT Legionella pneumophila strain Lp02

46.296

95.294

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

94.706

0.403
  pilU Vibrio cholerae strain A1552

38.298

96.765

0.371
  pilU Acinetobacter baylyi ADP1

37.015

98.529

0.365

Multiple sequence alignment