Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBT00_RS09035 Genome accession   NZ_CP045611
Coordinates   1910201..1911181 (+) Length   326 a.a.
NCBI ID   WP_022651830.1    Uniprot ID   A0AAE8X1F8
Organism   Enterobacter hormaechei strain AUH-ENM30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1905201..1916181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBT00_RS08995 - 1905231..1905557 (+) 327 WP_003862421.1 YggL family protein -
  GBT00_RS09000 - 1905615..1906331 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  GBT00_RS09005 - 1906444..1906806 (-) 363 WP_022651832.1 DUF559 domain-containing protein -
  GBT00_RS09010 hemW 1906863..1908011 (-) 1149 WP_003860019.1 radical SAM family heme chaperone HemW -
  GBT00_RS09015 - 1908004..1908597 (-) 594 WP_022651831.1 XTP/dITP diphosphatase -
  GBT00_RS09020 yggU 1908601..1908897 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  GBT00_RS09025 - 1908894..1909460 (-) 567 WP_003860024.1 YggT family protein -
  GBT00_RS09030 - 1909482..1910183 (-) 702 WP_003860026.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBT00_RS09035 pilT 1910201..1911181 (+) 981 WP_022651830.1 type IV pilus twitching motility protein PilT Machinery gene
  GBT00_RS09040 ruvX 1911191..1911607 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  GBT00_RS09045 - 1911607..1912167 (-) 561 WP_003860031.1 YqgE/AlgH family protein -
  GBT00_RS09050 gshB 1912242..1913189 (-) 948 WP_003860032.1 glutathione synthase -
  GBT00_RS09055 rsmE 1913221..1913952 (-) 732 WP_022651829.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GBT00_RS09060 endA 1914004..1914711 (-) 708 WP_022651828.1 deoxyribonuclease I -
  GBT00_RS09065 - 1914806..1915303 (-) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35653.57 Da        Isoelectric Point: 6.2335

>NTDB_id=395670 GBT00_RS09035 WP_022651830.1 1910201..1911181(+) (pilT) [Enterobacter hormaechei strain AUH-ENM30]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFSEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=395670 GBT00_RS09035 WP_022651830.1 1910201..1911181(+) (pilT) [Enterobacter hormaechei strain AUH-ENM30]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTTCTCAATCACCAGACAGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTTCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGTGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGTGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAATCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377

Multiple sequence alignment