Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GYMC52_RS13355 Genome accession   NC_014915
Coordinates   2709751..2710806 (-) Length   351 a.a.
NCBI ID   WP_013524244.1    Uniprot ID   -
Organism   Geobacillus sp. Y412MC52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704751..2715806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYMC52_RS13325 (GYMC52_2658) - 2704893..2705612 (-) 720 WP_013524240.1 hypothetical protein -
  GYMC52_RS13330 (GYMC52_2659) - 2705609..2706184 (-) 576 WP_013144607.1 fimbrial protein -
  GYMC52_RS13335 (GYMC52_2660) pilM 2706174..2707112 (-) 939 WP_013144606.1 type IV pilus biogenesis protein PilM -
  GYMC52_RS13340 (GYMC52_2661) - 2707134..2707889 (-) 756 WP_013524241.1 prepilin peptidase -
  GYMC52_RS13345 (GYMC52_2662) - 2707949..2708467 (-) 519 WP_013524242.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GYMC52_RS13350 (GYMC52_2663) - 2708553..2709764 (-) 1212 WP_013524243.1 type II secretion system F family protein -
  GYMC52_RS13355 (GYMC52_2664) pilT 2709751..2710806 (-) 1056 WP_013524244.1 type IV pilus twitching motility protein PilT Machinery gene
  GYMC52_RS13360 (GYMC52_2665) - 2710819..2712480 (-) 1662 WP_013524245.1 GspE/PulE family protein -
  GYMC52_RS13365 (GYMC52_2666) - 2712514..2713842 (-) 1329 WP_013524246.1 VanW family protein -
  GYMC52_RS13370 (GYMC52_2667) - 2713859..2714638 (-) 780 WP_013144599.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38467.46 Da        Isoelectric Point: 6.7270

>NTDB_id=39383 GYMC52_RS13355 WP_013524244.1 2709751..2710806(-) (pilT) [Geobacillus sp. Y412MC52]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVNGELRAYGHERLHPEETEQMAKAVIPAPLWQQFQETGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPVRIPTLEELGLPDVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNRHKTGRTAALEILVNNAAVANLIRNEKEHQIINIMQTSRSIGMQTME
MSVKELAADGRIDPLAAEVYLAGERGDDGAV

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=39383 GYMC52_RS13355 WP_013524244.1 2709751..2710806(-) (pilT) [Geobacillus sp. Y412MC52]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCGGCCTTTGAATGCCGCGCTTCCGACGTGCATTTGACCGTCGGTGCGCC
GCCGATTTTTCGCGTGAACGGGGAGTTGAGAGCTTACGGCCATGAGCGCCTGCACCCGGAGGAAACAGAGCAAATGGCGA
AAGCCGTCATTCCGGCGCCGCTTTGGCAGCAGTTTCAGGAAACCGGAGAGCTTGATTTTTCGTATGGCATTGCCGGCGTT
TCCCGCTTTCGCTTAAACGTGTTTAAACAGCGGTCATGCGTGTCGCTCGCCGTGCGCCTCATCCCGGTGCGCATCCCGAC
GCTGGAGGAGCTTGGCCTGCCGGATGTTTTAAAGCGCATCGCCGAAAAGCCGCACGGACTCGTGCTTGTCACCGGACCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAACATATCATCACGCTG
GAAGATCCAATCGAGTATTTGCATAAACACGGGAAATCGATCATCGACCAACGCGAGGTGGGATTCGACACTGGCAGTTT
TGCCGCCGGATTGCGCGCTGCGCTTCGTCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACAATCC
AAACGGCAATCACCGCCGCGGAAACGGGCCATTTAGTGCTTGGCACGCTTCATACGGCGAGCGCGCCGGCGGCGATTGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCGCAAATCCGGCTGCAGCTTGCTTCCGTGCTCGTTGCCATCATCGC
TCAGCGCTTGTTTCCAAACAGACACAAAACCGGGCGGACGGCAGCGCTGGAAATTTTAGTGAACAATGCGGCCGTCGCCA
ACTTAATCCGCAACGAAAAAGAACATCAAATCATTAACATTATGCAAACGAGCCGCAGCATCGGCATGCAGACGATGGAA
ATGAGCGTTAAGGAGCTTGCAGCCGATGGACGCATCGATCCGCTGGCGGCTGAAGTGTATTTGGCGGGGGAGCGTGGGGA
CGATGGCGCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.994

89.174

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.08

92.877

0.456
  pilT Vibrio cholerae strain A1552

49.08

92.877

0.456
  pilT Neisseria gonorrhoeae MS11

48.182

94.017

0.453
  pilT Neisseria meningitidis 8013

48.182

94.017

0.453
  pilT Pseudomonas aeruginosa PAK

48.916

92.023

0.45
  pilT Acinetobacter baylyi ADP1

48.16

92.877

0.447
  pilT Acinetobacter baumannii strain A118

47.273

94.017

0.444
  pilT Acinetobacter baumannii D1279779

47.273

94.017

0.444
  pilT Acinetobacter nosocomialis M2

46.97

94.017

0.442
  pilT Pseudomonas stutzeri DSM 10701

47.678

92.023

0.439
  pilT Legionella pneumophila strain ERS1305867

47.962

90.883

0.436
  pilT Legionella pneumophila strain Lp02

47.962

90.883

0.436
  pilU Vibrio cholerae strain A1552

41.127

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

43.769

93.732

0.41

Multiple sequence alignment