Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GCS91_RS28650 Genome accession   NZ_CP045291
Coordinates   6227434..6228570 (+) Length   378 a.a.
NCBI ID   WP_016450725.1    Uniprot ID   A0AAX3SP23
Organism   Delftia tsuruhatensis strain TR1180     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6222434..6233570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCS91_RS28620 (GCS91_28620) - 6222637..6223920 (+) 1284 WP_154835985.1 branched-chain amino acid ABC transporter substrate-binding protein -
  GCS91_RS28625 (GCS91_28625) - 6223959..6224684 (-) 726 WP_154835988.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCS91_RS28630 (GCS91_28630) pilT 6224722..6225765 (+) 1044 WP_016450723.1 type IV pilus twitching motility protein PilT Machinery gene
  GCS91_RS28635 (GCS91_28635) - 6225797..6226429 (+) 633 WP_017406039.1 cyclic nucleotide-binding domain-containing protein -
  GCS91_RS28640 (GCS91_28640) - 6226435..6227242 (-) 808 Protein_5632 IS5 family transposase -
  GCS91_RS28650 (GCS91_28650) pilU 6227434..6228570 (+) 1137 WP_016450725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GCS91_RS28655 (GCS91_28655) - 6228671..6229582 (+) 912 WP_016450726.1 NAD(P)-dependent oxidoreductase -
  GCS91_RS28660 (GCS91_28660) - 6229782..6230513 (-) 732 WP_154835992.1 BON domain-containing protein -
  GCS91_RS28665 (GCS91_28665) - 6230510..6231118 (-) 609 WP_016451992.1 SIS domain-containing protein -
  GCS91_RS28670 (GCS91_28670) - 6231192..6231608 (-) 417 WP_013804432.1 YraN family protein -
  GCS91_RS28675 (GCS91_28675) rsmI 6231608..6232549 (+) 942 WP_012202992.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42077.62 Da        Isoelectric Point: 6.9079

>NTDB_id=393132 GCS91_RS28650 WP_016450725.1 6227434..6228570(+) (pilU) [Delftia tsuruhatensis strain TR1180]
MERDQATKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGRITKVSPQPLSPVHTLTLARSVMSDKQIADFERTKECNFAI
SPPGIGRFRVNAFIQQGRVGMVMRTIPLALPTIDSLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIVTIEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDLSLNLRAMVSQRLLPKQDSKGRVAAIEVMLNSPLISDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLGSQRAKSLDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=393132 GCS91_RS28650 WP_016450725.1 6227434..6228570(+) (pilU) [Delftia tsuruhatensis strain TR1180]
ATGGAAAGAGATCAGGCCACCAAGTTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACGGCGGAATTCCCGCCGGCGATCAAGATCGACGGGCGCATCACCAAGGTGTCGCCGCAGCCGCTGTCGCCGGTGCACA
CGCTGACGCTGGCGCGCTCCGTCATGAGCGACAAGCAGATCGCCGATTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCGCCGGGCATAGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGATGCGGACGATTCC
GCTGGCCCTGCCCACCATCGACAGCCTGGGCGTACCCCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAATTCCTACGGC
CACATCGTGACCATCGAGGACCCCATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAGGTGGGCCT
GGACACGGACAGCTGGGAAGCAGCGCTCAAGAACTCCCTGCGCCAGGCACCGGACGTGATCCTCATGGGCGAAATCCGGG
ACCGCGAGACCATGGAGCACGCCATCGCCTTCTCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGCCGCCCGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCCCAGCGCCTGCTGCCCAAGCAGGACAGCAAGGGGCGCGTGGCCGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTCACCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGTCCAACCTCATCACCTACGAGGATGCCCTGCGCAATGCGGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGTCGCTGGACCTGGCGGCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.574

84.392

0.368
  pilT Vibrio cholerae strain A1552

43.574

84.392

0.368
  pilT Acinetobacter baylyi ADP1

42.073

86.772

0.365

Multiple sequence alignment