Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GCS91_RS28630 Genome accession   NZ_CP045291
Coordinates   6224722..6225765 (+) Length   347 a.a.
NCBI ID   WP_016450723.1    Uniprot ID   -
Organism   Delftia tsuruhatensis strain TR1180     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6219722..6230765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCS91_RS28610 (GCS91_28610) - 6220130..6221728 (-) 1599 WP_154835983.1 AMP-binding protein -
  GCS91_RS28615 (GCS91_28615) - 6221888..6222610 (+) 723 WP_016446678.1 TetR/AcrR family transcriptional regulator -
  GCS91_RS28620 (GCS91_28620) - 6222637..6223920 (+) 1284 WP_154835985.1 branched-chain amino acid ABC transporter substrate-binding protein -
  GCS91_RS28625 (GCS91_28625) - 6223959..6224684 (-) 726 WP_154835988.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCS91_RS28630 (GCS91_28630) pilT 6224722..6225765 (+) 1044 WP_016450723.1 type IV pilus twitching motility protein PilT Machinery gene
  GCS91_RS28635 (GCS91_28635) - 6225797..6226429 (+) 633 WP_017406039.1 cyclic nucleotide-binding domain-containing protein -
  GCS91_RS28640 (GCS91_28640) - 6226435..6227242 (-) 808 Protein_5632 IS5 family transposase -
  GCS91_RS28650 (GCS91_28650) pilU 6227434..6228570 (+) 1137 WP_016450725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GCS91_RS28655 (GCS91_28655) - 6228671..6229582 (+) 912 WP_016450726.1 NAD(P)-dependent oxidoreductase -
  GCS91_RS28660 (GCS91_28660) - 6229782..6230513 (-) 732 WP_154835992.1 BON domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38323.05 Da        Isoelectric Point: 6.8352

>NTDB_id=393131 GCS91_RS28630 WP_016450723.1 6224722..6225765(+) (pilT) [Delftia tsuruhatensis strain TR1180]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRIAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=393131 GCS91_RS28630 WP_016450723.1 6224722..6225765(+) (pilT) [Delftia tsuruhatensis strain TR1180]
GTGGACATCACCCAATTGCTGGCTTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGGCTTCCGCCGAT
GATCCGCGTGCACGGCGATGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAGACGGTCCACGCCATGGTCTACGACA
TCATGAGCGACTCCCAGCGCAAGGCCTACGAGGAGTTCCTGGAGGTGGATTTCTCGTTCGAGATCGAGGGGCTGGCGCGC
TTCCGTGTCAACGCCTTCAACCAGTACCGGGGCGCGGCCGCCGTCTTCCGGACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCGCGCATCTTCGCCGACCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAGGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACACAGTCGTTCTCGGC
CGCACTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CCATGACCGCAGCCGAGACGGGCCACCTGGTCTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCGGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTTTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCATCGCGGCCCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCCCAGATGTACTCCACCATCCAGACGAGTCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGATCTGGTGCGGCGCAACCTGATCAGCCCGGCCGAAGCGCGCGCCAAGGCCAAGATACCGGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

68.235

97.983

0.669
  pilT Neisseria gonorrhoeae MS11

67.941

97.983

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment