Detailed information NTG0000659

Experimentally validated

Overview

• Name: hexB
• Type: Machinery gene
• Locus tag: SPR_RS00865
• Genome accession: NC_003098
• Coordinates: 168072..170021 (+)
• Length: 649 a.a.
• NCBI ID: WP_000018169.1
• Uniprot ID: P0A3R1
• Organism: Streptococcus pneumoniae R6
• Function: DNA mismatch repair Homologous recombination

Function

Generation of a mutator phenotype in S. pneumoniae through deletions of mutX, hexA or hexB enhanced the expression of competence.

Genomic Context

Location: 163072..175021

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS00840 (spr0155)	-	163875..164408 (+)	534	WP_000729030.1	ABC transporter permease	-
SPR_RS00845 (spr0156)	nrdI	164486..164956 (+)	471	WP_000849624.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
SPR_RS00850 (spr0157)	-	165249..166511 (-)	1263	WP_000075798.1	NRAMP family divalent metal transporter	-
SPR_RS00855 (spr0158)	-	166821..167279 (-)	459	WP_000745054.1	DUF3021 domain-containing protein	-
SPR_RS00860 (spr0159)	-	167276..167716 (-)	441	WP_000861965.1	LytTR family DNA-binding domain-containing protein	-
SPR_RS00865 (spr0160)	hexB	168072..170021 (+)	1950	WP_000018169.1	DNA mismatch repair endonuclease MutL	Machinery gene
SPR_RS00870 (spr0161)	ribE	170346..170813 (-)	468	WP_001099502.1	6,7-dimethyl-8-ribityllumazine synthase	-
SPR_RS00875 (spr0162)	-	170814..172019 (-)	1206	WP_000456640.1	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	-
SPR_RS00880 (spr0163)	ribE	172039..172674 (-)	636	WP_000493840.1	riboflavin synthase	-
SPR_RS00885 (spr0164)	ribD	172659..173759 (-)	1101	WP_001284101.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
SPR_RS00890 (spr0165)	ruvA	174163..174756 (+)	594	WP_000271489.1	Holliday junction branch migration protein RuvA	Machinery gene

Sequence

Protein

• Length: 649 a.a.
• Molecular weight: 73434.89 Da
• Isoelectric Point: 5.3001

>NTDB_id=659 SPR_RS00865 WP_000018169.1 168072..170021(+) (hexB) [Streptococcus pneumoniae R6]
MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQIIIEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSK
IKNQADLFRIRTLGFRGEALPSIASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSAKKMIEIENSDLDFEISGFVSLP
ELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKLMVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSE
AIANSLKEQTLIPDALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLTLFAKETLD
RLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELEFFGQMHGTYLFAQGRDGLYIIDQHAAQERV
KYEEYRESIGNVDQSQQQLLVPYIFEFPADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCD
MLLLTKEVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHFTKSDMEKMFRRIQENH
TSLRELGKY

Nucleotide

• Length: 1950 bp

>NTDB_id=659 SPR_RS00865 WP_000018169.1 168072..170021(+) (hexB) [Streptococcus pneumoniae R6]
ATGTCTCATATTATTGAATTGCCAGAGATGCTGGCAAACCAAATCGCAGCTGGAGAGGTCATTGAACGTCCTGCCAGTGT
GGTCAAAGAGTTGGTAGAAAATGCCATTGACGCGGGCTCTAGTCAGATTATTATTGAGATTGAGGAAGCTGGTCTCAAGA
AGGTTCAAATCACGGATAACGGTCATGGAATTGCCCACGATGAGGTGGAGTTGGCCCTGCGTCGCCATGCGACCAGTAAG
ATAAAAAATCAAGCAGATCTCTTTCGGATTCGGACGCTTGGTTTTCGTGGTGAAGCCTTGCCTTCTATTGCGTCTGTTAG
TGTCTTGACTCTGTTAACGGCGGTGGATGGTGCTAGTCATGGAACCAAGTTAGTCGCGCGTGGGGGTGAAGTTGAGGAAG
TCATCCCAGCGACTAGTCCTGTGGGAACCAAGGTTTGTGTGGAGGATCTCTTTTTCAACACGCCTGCCCGTCTCAAGTAT
ATGAAGAGCCAGCAAGCGGAGTTGTCTCATATCATTGATATTGTCAACCGTCTGGGCTTGGCCCATCCTGAGATTTCTTT
TAGCTTGATTAGTGATGGCAAGGAAATGACGCGGACAGCAGGGACTGGTCAATTGCGCCAAGCAATCGCAGGGATTTACG
GTTTGGTCAGTGCCAAGAAGATGATTGAAATTGAGAACTCTGACCTAGATTTCGAAATTTCAGGTTTTGTGTCCTTGCCT
GAGTTGACTCGGGCTAACCGCAATTATATCAGTCTCTTCATCAATGGCCGTTATATCAAGAACTTCCTGCTCAATCGTGC
TATTTTGGATGGTTTTGGAAGCAAGCTTATGGTTGGACGTTTTCCACTGGCTGTCATTCACATCCATATCGACCCTTATC
TAGCGGATGTCAATGTGCATCCAACTAAGCAAGAGGTGCGGATTTCCAAGGAAAAAGAACTGATGACTCTGGTTTCAGAA
GCTATTGCAAATAGTCTCAAGGAACAAACCTTGATTCCAGATGCCTTGGAAAATCTTGCCAAATCGACCGTGCGCAATCG
TGAGAAGGTGGAGCAAACTATTCTCCCACTCAAAGAAAATACGCTCTACTATGAGAAAACTGAGCCGTCAAGACCTAGTC
AAACTGAAGTAGCTGATTATCAGGTAGAATTGACTGATGAAGGGCAGGATTTGACCCTGTTTGCCAAGGAAACCTTGGAC
CGATTGACCAAGCCAGCAAAACTGCATTTTGCAGAGAGAAAGCCTGCTAACTACGACCAGCTAGACCATCCAGAGTTAGA
TCTTGCTAGCATCGATAAGGCTTATGACAAACTGGAGCGAGAAGAAGCATCCAGCTTCCCAGAATTGGAGTTTTTCGGAC
AAATGCACGGGACTTATCTCTTTGCCCAAGGGCGAGATGGACTTTACATCATAGATCAGCACGCTGCTCAGGAACGGGTC
AAGTACGAGGAGTACCGTGAAAGCATTGGCAATGTTGACCAGAGCCAGCAGCAACTCCTAGTGCCCTATATCTTTGAATT
TCCTGCGGATGATGCCCTGCGTCTCAAGGAAAGAATGCCTCTCTTAGAGGAAGTGGGCGTCTTTCTAGCAGAGTACGGAG
AAAATCAATTTATTCTACGTGAACATCCTATTTGGATGGCAGAAGAAGAGATTGAATCAGGCATCTATGAGATGTGCGAC
ATGCTCCTTTTGACCAAGGAAGTTTCTATCAAGAAATACCGAGCAGAGCTGGCTATCATGATGTCTTGCAAGCGATCTAT
CAAGGCCAATCATCGTATTGATGATCATTCAGCTAGACAACTCCTCTATCAGCTTTCTCAATGTGACAATCCCTATAACT
GTCCTCACGGACGTCCTGTTTTGGTGCATTTTACCAAGTCGGATATGGAAAAGATGTTCCGACGTATTCAGGAAAATCAC
ACCAGTCTCCGTGAGTTGGGGAAATATTAA

Domains

Predicted by InterProScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3) (25-107)

• MutL C terminal dimerisation domain (MutL_C) (450-592)

• DNA mismatch repair protein, C-terminal domain (DNA_mis_repair) (209-326)

3D structure

• AlphaFold DB: P0A3R1

References

• [1]: Alyssa L Gagne et al. (2013) Competence in Streptococcus pneumoniae is a response to an increasing mutational burden. PloS One 8(8):e72613. [PMID: 23967325]
• [2]: O Humbert et al. (1995) Homeologous recombination and mismatch repair during transformation in Streptococcus pneumoniae: saturation of the Hex mismatch repair system. Proceedings of The National Academy of Sciences of The United States of America 92(20):9052-6. [PMID: 7568071]
• [3]: H Prats et al. (1985) The hexB mismatch repair gene of Streptococcus pneumoniae: characterisation, cloning and identification of the product. Molecular & General Genetics : MGG 200(3):482-9. [PMID: 2995767]