Detailed information    

experimental Experimentally validated

Overview

Name   ruvA   Type   Machinery gene
Locus tag   SPR_RS00890 Genome accession   NC_003098
Coordinates   174163..174756 (+) Length   197 a.a.
NCBI ID   WP_000271489.1    Uniprot ID   Q8DRE8
Organism   Streptococcus pneumoniae R6     
Function   homologous recombination   
Homologous recombination

Function

The RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex.


Genomic Context

Location: 169163..179756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS00870 (spr0161) ribE 170346..170813 (-) 468 WP_001099502.1 6,7-dimethyl-8-ribityllumazine synthase -
  SPR_RS00875 (spr0162) - 170814..172019 (-) 1206 WP_000456640.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  SPR_RS00880 (spr0163) ribE 172039..172674 (-) 636 WP_000493840.1 riboflavin synthase -
  SPR_RS00885 (spr0164) ribD 172659..173759 (-) 1101 WP_001284101.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
  SPR_RS00890 (spr0165) ruvA 174163..174756 (+) 594 WP_000271489.1 Holliday junction branch migration protein RuvA Machinery gene
  SPR_RS00895 (spr0166) - 174766..175329 (+) 564 WP_000166586.1 DNA-3-methyladenine glycosylase I -
  SPR_RS00900 (spr0167) - 175326..176003 (+) 678 WP_000987422.1 CPBP family intramembrane glutamic endopeptidase -
  SPR_RS00905 (spr0168) - 176158..177189 (-) 1032 WP_000255098.1 S66 family peptidase -
  SPR_RS00910 (spr0169) - 177444..178127 (-) 684 WP_000078023.1 DUF1129 domain-containing protein -
  SPR_RS00915 (spr0170) - 178139..179083 (-) 945 WP_000815629.1 magnesium transporter CorA family protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21548.06 Da        Isoelectric Point: 7.3085

>NTDB_id=682 SPR_RS00890 WP_000271489.1 174163..174756(+) (ruvA) [Streptococcus pneumoniae R6]
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=682 SPR_RS00890 WP_000271489.1 174163..174756(+) (ruvA) [Streptococcus pneumoniae R6]
ATGTACGCATATTTAAAAGGAATCATTACCAAAATTACTGCCAAATACATTGTTCTTGAAACCAATGGTATTGGTTATAT
CCTGCATGTGGCCAATCCTTATGCCTATTCAGGTCAGGTTAATCAGGAGGCTCAGATTTATGTGCATCAGGTTGTGCGTG
AGGACGCCCATTTGCTTTATGGATTTCGCTCAGAGGATGAGAAAAAGCTCTTTCTTAGTCTAATTTCGGTCTCTGGGATT
GGTCCTGTATCAGCTCTTGCTATTATCGCTGCTGATGACAATGCTGGCTTGGTTCAAGCCATTGAAACCAAGAACATTAC
CTACTTGACCAAGTTCCCTAAAATTGGCAAGAAAACAGCCCAGCAGATGGTGCTGGACTTGGAAGGCAAGGTAGTAGTTG
CAGGAGATGGCCTTCCTGCCAAGGTCGCAGTGCAAGCAAGTGCTGAAAACCAAGAATTGGAAGAAGCTATGGAAGCCATG
TTGGCTCTGGGCTACAAGGCAACAGAGCTCAAGAAAATCAAGAAATTCTTTGAAGGAACGACAGATACAGCTGAGAACTA
TATCAAGTCGGCCCTTAAAATGTTGGTCAAATAG

Domains

Predicted by InterProScan.

Helix-hairpin-helix domain (HHH_5)

(71-127)

RuvA N terminal domain (RuvA_N)

(1-61)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DRE8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvA Streptococcus pneumoniae D39

100

100

1
  ruvA Streptococcus pneumoniae TIGR4

99.492

100

0.995
  ruvA Bacillus subtilis subsp. subtilis str. 168

39.806

100

0.416

References

[1] J Hoskins et al. (2001) Genome of the bacterium Streptococcus pneumoniae strain R6. Journal of Bacteriology 183(19):5709-17. [PMID: 11544234]