Detailed information    

experimental Experimentally validated

Overview

Name   ruvA   Type   Machinery gene
Locus tag   BSU_27740 Genome accession   NC_000964
Coordinates   2836165..2836770 (-) Length   201 a.a.
NCBI ID   NP_390652.1    Uniprot ID   O05392
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

The RuvAB and RecG branch migrating DNA helicases, the RecU Holliday junction (HJ) resolvase, and RecD bias HJ resolution towards noncrossovers.


Genomic Context

Location: 2831165..2841770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_27690 (BSU27690) yrzE 2831915..2832367 (+) 453 NP_390647.1 putative transporter -
  BSU_27700 (BSU27700) yrbF 2832424..2832690 (-) 267 NP_390648.2 component of the preprotein translocase -
  BSU_27710 (BSU27710) tgt 2832727..2833872 (-) 1146 NP_390649.1 tRNA-guanine transglycosylase -
  BSU_27720 (BSU27720) queA 2833899..2834927 (-) 1029 NP_390650.1 S-adenosylmethionine tRNA ribosyltransferase-isomerase -
  BSU_27729 (BSU27729) yrzS 2834957..2835157 (-) 201 YP_003097768.1 conserved membrane protein of unknown function -
  BSU_27730 (BSU27730) ruvB 2835150..2836154 (-) 1005 YP_054590.1 Holliday junction DNA helicase, ATP-dependent component Machinery gene
  BSU_27740 (BSU27740) ruvA 2836165..2836770 (-) 606 NP_390652.1 Holliday junction DNA helicase Machinery gene
  BSU_27750 (BSU27750) bofC 2836909..2837421 (-) 513 NP_390653.1 bypass of forespore C, intercompartmental signaling factor -
  BSU_27760 (BSU27760) csbX 2837469..2838776 (-) 1308 NP_390654.2 putative catecholate siderophose exporter -
  BSU_27770 (BSU27770) yrbE 2838847..2839872 (-) 1026 NP_390655.1 putative inositol-related oxidoreductase -
  BSU_27785 (BSU27785) yrzF 2840110..2840757 (+) 648 NP_390656.2 putative serine/threonine-protein kinase -
  BSU_27786 (BSU27786) - 2840803..2840925 (-) 123 YP_009513992.1 hypothetical protein -
  BSU_27800 (BSU27800) yrzH 2841010..2841300 (+) 291 NP_390658.2 hypothetical protein -
  BSU_27809 (BSU27809) yrzT 2841307..2841447 (+) 141 YP_003097769.1 hypothetical protein -

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 22584.08 Da        Isoelectric Point: 5.4072

>NTDB_id=632 BSU_27740 NP_390652.1 2836165..2836770(-) (ruvA) [Bacillus subtilis subsp. subtilis str. 168]
MIEFVKGTIDYVSPQYIVIENGGIGYQIFTPNPFIYKERSQETIFTYHHIREDAFSLYGFSTREEKALFTKLLNVTGIGP
KGALAILGSGDPGAVIQAIENEDEAFLVKFPGVGKKTARQIILDLKGKLADVVPEMIENLFNHEERLEKQTAETALEEAL
EALRVLGYAEKEIKKVLPHLKEEIGLTTDQYVKKALQKLLK

Nucleotide


Download         Length: 606 bp        

>NTDB_id=632 BSU_27740 NP_390652.1 2836165..2836770(-) (ruvA) [Bacillus subtilis subsp. subtilis str. 168]
GTGATTGAATTTGTTAAAGGGACGATTGATTATGTATCTCCCCAATATATTGTCATAGAGAACGGCGGCATCGGCTATCA
GATTTTCACGCCGAATCCGTTTATATATAAGGAGAGAAGCCAAGAAACGATTTTTACATACCATCATATCAGAGAGGATG
CTTTTTCGCTTTATGGTTTTTCAACAAGAGAGGAGAAGGCGCTCTTTACAAAGCTGTTAAACGTGACCGGAATTGGCCCG
AAAGGAGCACTTGCCATCTTGGGCTCGGGTGATCCCGGAGCGGTCATCCAAGCAATTGAAAATGAAGATGAAGCTTTTTT
GGTCAAGTTTCCTGGAGTAGGAAAGAAAACCGCACGCCAGATTATTCTAGATTTAAAAGGAAAGCTGGCAGATGTTGTGC
CTGAAATGATTGAAAATCTGTTTAATCATGAAGAAAGGCTTGAAAAGCAAACAGCGGAAACGGCTCTTGAAGAAGCACTT
GAAGCCCTCAGGGTTCTCGGATATGCTGAAAAAGAAATCAAAAAGGTGCTTCCTCACCTTAAAGAAGAAATTGGGCTGAC
AACAGACCAATATGTGAAAAAAGCATTACAAAAACTATTAAAGTAA

Domains

Predicted by InterProScan.

RuvA, C-terminal domain (RuvA_C)

(155-199)

RuvA N terminal domain (RuvA_N)

(1-59)

Helix-hairpin-helix domain (HHH_5)

(69-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O05392

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvA Streptococcus pneumoniae R6

39.806

100

0.416
  ruvA Streptococcus pneumoniae TIGR4

39.806

100

0.416
  ruvA Streptococcus pneumoniae D39

39.806

100

0.416

References

[1] Carolina Gándara et al. (2021) DisA Restrains the Processing and Cleavage of Reversed Replication Forks by the RuvAB-RecU Resolvasome. International Journal of Molecular Sciences 22(21):11323. [PMID: 34768753]
[2] Rubén Torres et al. (2017) Interplay between Bacillus subtilis RecD2 and the RecG or RuvAB helicase in recombinational repair. DNA Repair 55:40-46. [PMID: 28527403]
[3] Cristina Cañas et al. (2014) Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution. The Journal of Biological Chemistry 289(25):17634-46. [PMID: 24770420]
[4] Humberto Sanchez et al. (2005) The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. Genetics 171(3):873-83. [PMID: 16020779]
[5] Silvia Ayora et al. (2004) Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA. Proceedings of The National Academy of Sciences of The United States of America 101(2):452-7. [PMID: 14701911]
[6] Begoña Carrasco et al. (2004) Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation. Journal of Bacteriology 186(17):5557-66. [PMID: 15317759]