Detailed information    

experimental Experimentally validated

Overview

Name   ruvA   Type   Machinery gene
Locus tag   SP_RS00890 Genome accession   NC_003028
Coordinates   171843..172436 (+) Length   197 a.a.
NCBI ID   WP_000271487.1    Uniprot ID   Q97SY4
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

The RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex.


Genomic Context

Location: 166843..177436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS00870 (SP_0175) ribH 168025..168492 (-) 468 WP_001099502.1 6,7-dimethyl-8-ribityllumazine synthase -
  SP_RS00875 (SP_0176) - 168493..169698 (-) 1206 WP_001835921.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  SP_RS00880 (SP_0177) ribE 169718..170353 (-) 636 WP_000493842.1 riboflavin synthase -
  SP_RS00885 (SP_0178) ribD 170338..171438 (-) 1101 WP_001284098.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
  SP_RS00890 (SP_0179) ruvA 171843..172436 (+) 594 WP_000271487.1 Holliday junction branch migration protein RuvA Machinery gene
  SP_RS00895 (SP_0180) - 172446..173009 (+) 564 WP_000166604.1 DNA-3-methyladenine glycosylase I -
  SP_RS00900 (SP_0181) - 173006..173683 (+) 678 WP_000987408.1 CPBP family intramembrane glutamic endopeptidase -
  SP_RS00905 (SP_0182) - 173820..174851 (-) 1032 WP_000255096.1 S66 family peptidase -
  SP_RS00910 (SP_0184) - 175106..175789 (-) 684 WP_000078022.1 DUF1129 domain-containing protein -
  SP_RS00915 (SP_0185) - 175801..176745 (-) 945 WP_000815629.1 magnesium transporter CorA family protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21606.10 Da        Isoelectric Point: 6.7338

>NTDB_id=681 SP_RS00890 WP_000271487.1 171843..172436(+) (ruvA) [Streptococcus pneumoniae TIGR4]
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=681 SP_RS00890 WP_000271487.1 171843..172436(+) (ruvA) [Streptococcus pneumoniae TIGR4]
ATGTACGCATATTTAAAAGGAATCATTACCAAAATTACTGCCAAATACATTGTTCTTGAAACCAATGGTATTGGTTATAT
CCTGCATGTGGCCAATCCTTATGCCTATTCAGGTCAGGTTAATCAGGAGGCTCAGATTTATGTGCATCAGGTTGTGCGTG
AGGACGCCCATTTGCTTTATGGATTTCGCTCAGAGGATGAGAAAAAGCTCTTTCTTAGTCTGATTTCGGTCTCTGGGATT
GGTCCTGTATCAGCTCTTGCTATTATCGCTGCTGATGACAATGCTGGCTTGGTTCAAGCCATTGAAACCAAGAACATCAC
CTACTTGACCAAGTTCCCTAAAATTGGCAAGAAAACAGCCCAGCAGATGGTGCTGGACTTGGAAGGCAAGGTAGTAGTTG
CAGGAGATGACCTTCCTGCCAAGGTCGCAGTGCAAGCAAGTGCTGAAAACCAAGAATTGGAAGAAGCTATGGAAGCCATG
TTGGCTCTGGGCTACAAGGCAACAGAGCTCAAGAAAATCAAGAAATTCTTTGAAGGAACGACAGATACAGCTGAGAACTA
TATCAAGTCGGCCCTTAAAATGTTGGTCAAATAG

Domains

Predicted by InterProScan.

Helix-hairpin-helix domain (HHH_5)

(71-127)

RuvA N terminal domain (RuvA_N)

(1-61)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q97SY4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvA Streptococcus pneumoniae R6

99.492

100

0.995
  ruvA Streptococcus pneumoniae D39

99.492

100

0.995
  ruvA Bacillus subtilis subsp. subtilis str. 168

39.806

100

0.416

References

[1] H Tettelin et al. (2001) Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science (New York, N.Y.) 293(5529):498-506. [PMID: 11463916]