Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TMAR_RS03400 Genome accession   NC_014831
Coordinates   800268..801302 (+) Length   344 a.a.
NCBI ID   WP_013495084.1    Uniprot ID   E6SHT2
Organism   Thermaerobacter marianensis DSM 12885     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795268..806302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMAR_RS03375 (Tmar_0657) - 795463..796236 (+) 774 WP_013495080.1 NAD-dependent deacylase -
  TMAR_RS13100 (Tmar_0658) - 796356..796982 (+) 627 WP_013495081.1 hypothetical protein -
  TMAR_RS03385 (Tmar_0659) - 797249..797737 (+) 489 WP_013495082.1 DinB family protein -
  TMAR_RS03390 (Tmar_0660) - 798085..798348 (+) 264 WP_013495083.1 MoaD/ThiS family protein -
  TMAR_RS03395 (Tmar_0661) - 798402..799409 (+) 1008 WP_207635138.1 acetamidase/formamidase family protein -
  TMAR_RS13825 - 799453..799719 (+) 267 WP_207635139.1 hypothetical protein -
  TMAR_RS03400 (Tmar_0662) pilT 800268..801302 (+) 1035 WP_013495084.1 type IV pilus twitching motility protein PilT Machinery gene
  TMAR_RS03405 (Tmar_0663) - 801416..802339 (+) 924 WP_013495085.1 A24 family peptidase -
  TMAR_RS03410 (Tmar_0664) - 802538..804847 (+) 2310 WP_013495086.1 ATPase, T2SS/T4P/T4SS family -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37350.03 Da        Isoelectric Point: 6.7784

>NTDB_id=39266 TMAR_RS03400 WP_013495084.1 800268..801302(+) (pilT) [Thermaerobacter marianensis DSM 12885]
MKGPELAEWLRQAAEHGASDLHLTVESPPVVRVNGELADLPGPRLRPEDVQELVRPVLDARLQERLDREGQVDFAYSLPG
VGRFRANIYRQRGSLAAAFRLIPTTVPDLDELGLPPAVAGLCELSQGLVLVTGPTGSGKSTTLAAMIDRINRTRRVHIVT
LEDPIEYLHRHRLSLVHQREVGLDVPTFAAGLRAALREDPDVIMVGEMRDLETIAIALTAAETGHLVLATLHTPSAPQAV
DRIIDVFPAEQQNQVRVQLAGVLEGVVAQRLVPAADGRGRHVLAEVLLGTAAVRNLIREGKTAQLAAVMETSRQYGMVTL
RESAGQLLARGLVTREALLRVGVR

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=39266 TMAR_RS03400 WP_013495084.1 800268..801302(+) (pilT) [Thermaerobacter marianensis DSM 12885]
GTGAAAGGGCCCGAACTGGCCGAATGGTTGCGTCAGGCCGCCGAGCACGGCGCCTCCGACCTGCACCTGACGGTGGAATC
GCCCCCGGTGGTGCGGGTGAACGGGGAGCTGGCCGACCTGCCGGGACCTCGCCTGCGCCCGGAGGACGTCCAGGAGCTGG
TTCGTCCGGTGCTGGACGCTCGCCTCCAGGAGCGCCTGGACCGGGAGGGGCAGGTGGACTTCGCCTACAGCCTGCCCGGG
GTCGGCCGCTTCCGGGCTAACATCTACCGGCAGCGGGGCAGCCTGGCGGCCGCCTTCCGGCTGATCCCCACCACCGTTCC
CGACCTGGACGAGCTCGGCCTGCCGCCCGCCGTGGCCGGTCTCTGCGAGCTGTCCCAGGGGCTGGTGCTGGTCACCGGAC
CCACGGGCAGCGGGAAGTCGACCACCCTGGCGGCCATGATCGACCGGATCAACCGCACCCGCCGGGTTCACATCGTGACG
CTGGAAGACCCCATCGAGTACCTGCATCGCCACCGCCTGAGCCTGGTCCACCAGCGGGAGGTGGGCCTCGACGTGCCCAC
CTTCGCCGCCGGGCTGCGCGCGGCCCTGCGGGAAGACCCCGACGTGATCATGGTCGGGGAGATGCGGGACCTGGAGACCA
TCGCCATCGCCCTGACGGCGGCGGAGACGGGGCACCTGGTGCTGGCGACCCTGCACACGCCCAGCGCCCCCCAGGCGGTG
GACCGGATCATCGACGTCTTCCCCGCGGAGCAGCAGAACCAGGTGCGGGTGCAGCTGGCGGGGGTGCTGGAAGGCGTGGT
GGCCCAGCGGCTGGTGCCGGCGGCCGACGGGCGGGGCCGGCACGTCCTGGCCGAGGTGCTACTCGGGACGGCGGCGGTGC
GGAACCTGATCCGCGAGGGGAAGACGGCCCAGCTGGCGGCGGTGATGGAGACCTCCCGCCAGTACGGCATGGTGACCCTG
CGGGAGAGCGCCGGCCAGCTGCTGGCCCGGGGCCTGGTGACGCGGGAGGCGCTGCTGCGGGTGGGGGTGCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6SHT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.149

97.384

0.488
  pilT Vibrio cholerae strain A1552

48.328

95.64

0.462
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.328

95.64

0.462
  pilT Acinetobacter baylyi ADP1

47.879

95.93

0.459
  pilT Neisseria gonorrhoeae MS11

47.024

97.674

0.459
  pilT Acinetobacter baumannii D1279779

47.576

95.93

0.456
  pilT Acinetobacter baumannii strain A118

47.576

95.93

0.456
  pilT Pseudomonas aeruginosa PAK

47.289

96.512

0.456
  pilT Neisseria meningitidis 8013

47.289

96.512

0.456
  pilT Acinetobacter nosocomialis M2

47.273

95.93

0.453
  pilT Pseudomonas stutzeri DSM 10701

46.547

96.802

0.451
  pilT Legionella pneumophila strain Lp02

46.224

96.221

0.445
  pilT Legionella pneumophila strain ERS1305867

46.224

96.221

0.445
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

42.593

94.186

0.401

Multiple sequence alignment