Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SULKU_RS05895 Genome accession   NC_014762
Coordinates   1166281..1167351 (-) Length   356 a.a.
NCBI ID   WP_013460030.1    Uniprot ID   E4TWZ2
Organism   Sulfuricurvum kujiense DSM 16994     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1161281..1172351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SULKU_RS05870 (Sulku_1165) - 1162225..1162707 (-) 483 WP_013460025.1 Fur family transcriptional regulator -
  SULKU_RS05875 (Sulku_1166) - 1162711..1163262 (-) 552 WP_013460026.1 CvpA family protein -
  SULKU_RS05880 (Sulku_1167) - 1163262..1164188 (-) 927 WP_013460027.1 GGDEF domain-containing protein -
  SULKU_RS05885 (Sulku_1168) - 1164394..1165740 (+) 1347 WP_013460028.1 3-deoxy-7-phosphoheptulonate synthase class II -
  SULKU_RS05890 (Sulku_1169) - 1165773..1166177 (-) 405 WP_013460029.1 Fur family transcriptional regulator -
  SULKU_RS05895 (Sulku_1170) pilT 1166281..1167351 (-) 1071 WP_013460030.1 type IV pilus twitching motility protein PilT Machinery gene
  SULKU_RS05900 (Sulku_1171) - 1167348..1167692 (-) 345 WP_013460031.1 arsenate reductase family protein -
  SULKU_RS05905 (Sulku_1172) gatC 1167696..1167986 (-) 291 WP_013460032.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  SULKU_RS05910 (Sulku_1173) - 1168095..1168706 (+) 612 WP_049766960.1 hypothetical protein -
  SULKU_RS05915 (Sulku_1174) - 1168706..1169704 (+) 999 WP_013460034.1 hypothetical protein -
  SULKU_RS05920 (Sulku_1175) - 1169704..1170525 (+) 822 WP_013460035.1 tyrosine-type recombinase/integrase -
  SULKU_RS05925 (Sulku_1176) - 1170590..1170931 (+) 342 WP_013460036.1 cupin domain-containing protein -
  SULKU_RS05930 (Sulku_1177) - 1170939..1171556 (+) 618 WP_013460037.1 type III pantothenate kinase -
  SULKU_RS05935 (Sulku_1178) hisG 1171550..1172167 (+) 618 WP_013460038.1 ATP phosphoribosyltransferase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39815.77 Da        Isoelectric Point: 6.6196

>NTDB_id=39129 SULKU_RS05895 WP_013460030.1 1166281..1167351(-) (pilT) [Sulfuricurvum kujiense DSM 16994]
MNTTLDIYKLLKSVVAYKASDLHLVSRSEPQIRIDGRLVALNLPVLTGQDIEEMGYSLLTEKQKKAFEENNELDFSIVIP
DVGRFRANYYKTMDEVACAFRIIPVIIPSLDDLNAKKVFKELIKREKGLILVTGPTGSGKSTTLAAMLNEINMNEARHII
TVEDPVEFVHNNKKSLFSHRNVGTDTKSFSNALKYALREDPDVILIGEMRDRETISAALTAAETGHLVFGTLHTNSAPQT
INRIIDVFSGDEQPQVRAQLSTSLIAVISQALLPKIGGGRVAAQEIMITNPAIANLIREDKVHQLYSQMQLNQQETNMTT
QTQEIVDFLRKKLINKETALQYSNKPDELVRIINSL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=39129 SULKU_RS05895 WP_013460030.1 1166281..1167351(-) (pilT) [Sulfuricurvum kujiense DSM 16994]
ATGAATACCACGTTGGATATTTACAAGCTTCTTAAAAGCGTTGTGGCGTACAAGGCATCCGATTTACATCTTGTCAGCCG
TTCAGAACCGCAGATTCGCATCGACGGACGCCTTGTCGCCCTGAACCTCCCCGTACTAACCGGGCAGGATATCGAAGAGA
TGGGATATTCTCTCCTCACCGAGAAGCAAAAAAAAGCGTTTGAAGAGAATAATGAACTTGACTTTTCGATTGTCATTCCC
GATGTCGGCCGTTTCCGTGCCAACTACTACAAAACCATGGACGAAGTCGCCTGTGCGTTTCGTATCATCCCCGTCATTAT
CCCCTCTTTGGATGATTTGAACGCCAAAAAAGTATTTAAAGAGCTGATTAAACGCGAAAAAGGGCTAATCCTTGTCACCG
GACCGACGGGAAGTGGTAAATCGACCACCCTTGCGGCGATGCTGAACGAAATCAACATGAACGAAGCCCGCCATATCATC
ACGGTCGAAGATCCGGTCGAGTTTGTCCATAACAACAAAAAATCGCTCTTCTCCCATCGTAATGTCGGAACCGATACCAA
AAGCTTCTCGAACGCACTGAAATACGCTCTGCGTGAAGACCCGGACGTTATCCTGATCGGGGAGATGCGGGATCGCGAAA
CGATTTCAGCGGCGTTGACGGCGGCAGAAACGGGACACTTGGTCTTCGGAACACTCCACACCAACTCGGCACCGCAAACC
ATCAACCGTATCATCGACGTTTTCTCGGGTGATGAACAGCCGCAGGTACGGGCTCAATTGTCTACCTCCCTGATCGCCGT
CATATCCCAAGCCCTTTTGCCGAAAATCGGCGGCGGACGTGTCGCTGCGCAGGAGATTATGATCACCAATCCGGCGATTG
CCAATCTGATCCGCGAAGACAAAGTCCATCAGCTCTATTCGCAAATGCAGCTCAATCAGCAAGAGACCAATATGACGACC
CAGACACAGGAAATCGTCGACTTCCTGCGCAAAAAACTGATCAACAAAGAGACCGCTTTGCAATATTCCAACAAACCCGA
CGAATTGGTTCGTATCATCAATTCACTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TWZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.884

96.91

0.503
  pilT Acinetobacter nosocomialis M2

51.734

97.191

0.503
  pilT Acinetobacter baumannii D1279779

51.734

97.191

0.503
  pilT Acinetobacter baumannii strain A118

51.734

97.191

0.503
  pilT Legionella pneumophila strain Lp02

52.836

94.101

0.497
  pilT Legionella pneumophila strain ERS1305867

52.836

94.101

0.497
  pilT Pseudomonas stutzeri DSM 10701

51.603

96.348

0.497
  pilT Pseudomonas aeruginosa PAK

51.312

96.348

0.494
  pilT Neisseria gonorrhoeae MS11

50.893

94.382

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.882

85.955

0.48
  pilT Vibrio cholerae strain A1552

55.882

85.955

0.48
  pilT Neisseria meningitidis 8013

50.595

94.382

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.07

99.719

0.449
  pilU Acinetobacter baylyi ADP1

39.481

97.472

0.385
  pilU Pseudomonas stutzeri DSM 10701

38

98.315

0.374

Multiple sequence alignment