Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAAJ005_RS03180 Genome accession   NZ_CP044990
Coordinates   234605..235846 (-) Length   413 a.a.
NCBI ID   WP_151845850.1    Uniprot ID   A0A5J6YTD3
Organism   Deinococcus sp. AJ005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 229605..240846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAAJ005_RS03165 (DAAJ005_03165) clpP 230137..230742 (+) 606 WP_188971500.1 ATP-dependent Clp protease proteolytic subunit -
  DAAJ005_RS03170 (DAAJ005_03170) clpX 230739..231953 (+) 1215 WP_151845848.1 ATP-dependent Clp protease ATP-binding subunit ClpX -
  DAAJ005_RS03175 (DAAJ005_03175) lon 232102..234537 (+) 2436 WP_151845849.1 endopeptidase La -
  DAAJ005_RS03180 (DAAJ005_03180) pilT 234605..235846 (-) 1242 WP_151845850.1 type IV pilus twitching motility protein PilT Machinery gene
  DAAJ005_RS03185 (DAAJ005_03185) pilB 235917..238595 (-) 2679 WP_151845851.1 type II/IV secretion system protein Machinery gene
  DAAJ005_RS03190 (DAAJ005_03190) - 238697..239203 (-) 507 WP_151845852.1 YqeG family HAD IIIA-type phosphatase -
  DAAJ005_RS03195 (DAAJ005_03195) pgeF 239200..239970 (-) 771 WP_151845853.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 44724.97 Da        Isoelectric Point: 8.0696

>NTDB_id=391136 DAAJ005_RS03180 WP_151845850.1 234605..235846(-) (pilT) [Deinococcus sp. AJ005]
MTQPSADITDILRTAAEKGASDVILTVGMAPQFKIDGVYGPQGFADLVPTETRKLMYSMMNEKQQRTFEERRELDFSFAL
GDRARFRVNCFMQRGNVGGVMRLIPTKIKSAAEMGLPQSVVDIANAPRGLVLVTGPTGSGKSTTLAAMLDYINANKKLHI
VTIEDPIEFMHSHKQSIVNQREVGSDTMSFENALRAALRQAPDVILVGEMRDHETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRLDGQGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMV
TMDAFLANLYRRRMITFDVGASRSVDPKEFARLANDAGGASAGNQPQAGYSPPPVSSATGKSTNWAEPGRAAATSTPETG
SYGGAASLGRSKR

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=391136 DAAJ005_RS03180 WP_151845850.1 234605..235846(-) (pilT) [Deinococcus sp. AJ005]
ATGACCCAGCCCTCCGCCGACATCACCGACATCCTGCGAACCGCCGCCGAGAAGGGCGCGTCCGACGTGATCCTGACCGT
GGGCATGGCCCCGCAGTTCAAGATCGACGGCGTGTACGGCCCGCAAGGTTTCGCCGATCTGGTGCCCACCGAGACCCGCA
AGCTGATGTACTCGATGATGAACGAGAAACAGCAGCGCACCTTCGAGGAACGCCGCGAGCTGGACTTTTCTTTCGCGCTG
GGGGACCGCGCCCGCTTCCGCGTCAACTGCTTCATGCAGCGCGGCAACGTGGGCGGCGTGATGCGCCTGATTCCCACCAA
GATCAAGAGCGCCGCCGAGATGGGACTGCCACAGAGCGTCGTCGATATCGCCAACGCGCCGCGCGGGCTGGTGCTGGTCA
CCGGGCCGACGGGTTCGGGGAAAAGCACCACGCTGGCGGCCATGCTGGATTACATCAACGCCAACAAGAAACTGCATATC
GTGACCATCGAAGACCCCATCGAGTTCATGCACTCACACAAGCAGAGCATCGTCAACCAGCGCGAGGTCGGCTCGGACAC
CATGAGCTTTGAAAATGCCCTGCGCGCGGCGCTGCGTCAGGCCCCCGACGTGATCCTGGTGGGGGAAATGCGTGACCACG
AGACGATCAAGGCCGCCGTGACCGCTGCCGAAACCGGGCATCTGGTGATGGGCACGCTGCACACCAACAGTGCCCCGGAA
TCCATCGACCGAATCGTGGACGTGTTCCCCGAAGAGCAGCAGGAACAGATCCGGGTGCAGCTCGCCAACAACCTGGTGGC
GGTGATGACCCAGCAGTTGCTGCCGCGTCTGGACGGGCAGGGCCGTATCCTGGCCTACGAGCTGCTGATCGCCAATCCCG
CCGTGCGCGCCCTGATCCGCGAGGGCAAAACGTACCAGATCACCAGCGTGATGCAGACTGGCGCACGCGAGGGCATGGTG
ACGATGGACGCCTTTCTGGCCAACCTGTACCGCCGCCGTATGATCACCTTCGATGTGGGGGCCTCACGTTCCGTCGATCC
CAAGGAATTTGCCCGCCTTGCCAACGACGCCGGGGGGGCTTCCGCCGGGAACCAGCCACAGGCTGGCTACAGCCCGCCGC
CTGTCTCGTCTGCAACGGGCAAAAGTACCAACTGGGCCGAGCCGGGAAGGGCCGCAGCCACCAGCACGCCAGAAACCGGA
AGCTACGGCGGCGCGGCCAGTCTGGGCAGAAGCAAGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6YTD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

74.447

98.547

0.734
  pilT Acinetobacter nosocomialis M2

51.163

83.293

0.426
  pilT Acinetobacter baumannii D1279779

51.163

83.293

0.426
  pilT Acinetobacter baumannii strain A118

51.163

83.293

0.426
  pilT Neisseria meningitidis 8013

51.32

82.567

0.424
  pilT Pseudomonas stutzeri DSM 10701

50.581

83.293

0.421
  pilT Acinetobacter baylyi ADP1

50.581

83.293

0.421
  pilT Pseudomonas aeruginosa PAK

50

83.293

0.416
  pilT Neisseria gonorrhoeae MS11

51.829

79.419

0.412
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.986

83.535

0.409
  pilT Vibrio cholerae strain A1552

48.986

83.535

0.409
  pilT Legionella pneumophila strain Lp02

49.091

79.903

0.392
  pilT Legionella pneumophila strain ERS1305867

49.091

79.903

0.392
  pilU Pseudomonas stutzeri DSM 10701

44.034

85.23

0.375

Multiple sequence alignment