Detailed information NTG0391137

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DAAJ005_RS03185
• Genome accession: NZ_CP044990
• Coordinates: 235917..238595 (-)
• Length: 892 a.a.
• NCBI ID: WP_151845851.1
• Uniprot ID: A0A5J6YPY9
• Organism: Deinococcus sp. AJ005
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 230917..243595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DAAJ005_RS03175 (DAAJ005_03175)	lon	232102..234537 (+)	2436	WP_151845849.1	endopeptidase La	-
DAAJ005_RS03180 (DAAJ005_03180)	pilT	234605..235846 (-)	1242	WP_151845850.1	type IV pilus twitching motility protein PilT	Machinery gene
DAAJ005_RS03185 (DAAJ005_03185)	pilB	235917..238595 (-)	2679	WP_151845851.1	type II/IV secretion system protein	Machinery gene
DAAJ005_RS03190 (DAAJ005_03190)	-	238697..239203 (-)	507	WP_151845852.1	YqeG family HAD IIIA-type phosphatase	-
DAAJ005_RS03195 (DAAJ005_03195)	pgeF	239200..239970 (-)	771	WP_151845853.1	peptidoglycan editing factor PgeF	-
DAAJ005_RS03200 (DAAJ005_03200)	-	240101..240886 (+)	786	WP_151845854.1	enoyl-ACP reductase	-
DAAJ005_RS03205 (DAAJ005_03205)	-	241358..241963 (+)	606	WP_051655150.1	response regulator transcription factor	-

Sequence

Protein

• Length: 892 a.a.
• Molecular weight: 97042.34 Da
• Isoelectric Point: 4.8703

>NTDB_id=391137 DAAJ005_RS03185 WP_151845851.1 235917..238595(-) (pilB) [Deinococcus sp. AJ005]
MALSIGDRRLGAILLEQGYVNDGDLQKALVRHSEVGGRLAEILIDTGLVGEKRIARAIEEALGIPLVNLLAITPDPAAVA
AIPAQTALNLQAIPFALENGVLRVALVDPLSSLALETLEDDSNLPIEPYQALREQVLWAVATHYPELGLTPVAPADFGEE
GPGGQLGQALIARGLVTDAQLQVALDAQQQTGEALGNTLVAQKAITEDQLYELLAEQSDVIYLKNPRDFQPAEDVLGSML
RADALRLSAVPVDETDEHVVVVTSDPRKRDDIAALIGRPIQLILARPRDLEFLTERFYPQRGRLGEQMVQEGTLSRQQLR
EALQVQARAGKVKPLGEVILDLGFADASAIDSALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
AKVALLIPEPTARRYGVVPVRLQGESLVIAMKDPRNVFALDDLKLITGRDIIPAVMAEKDIIRLIERYFGNQGMADLNQR
LQTESLQREKDAKRGNDVDVNAGFDDNAVVKVVDGLIREAALQEASDIHIEPTEFSLRIRYRVDGILREQNELPKGAAQS
ILARIKIMGNLDISERRVPQDGRVRFKKGSIDIDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSDHNFQRYLDVID
KPNGIFLITGPTGSGKSFTSFSTLKRIAKPEKNTTTIEDPVEYEIPGIIQTQVNPVAGLTFARALRAFLRQDPDIIFVGE
IRDTETAKIAVEAALTGHLVLATLHTNDAPGAIVRLEEMGVEPFNISASVVGVLAQRLVRKVCPDCKAPTNADPDVLRRL
GITDRDLRGAQLMRGAGCARCGGTGYKGRMGIHELMVVDEPLRVAIGTGKPATEIRDVALEKSGMKTLRQDGIEKALKGI
TTLEEVLAVTSN

Nucleotide

• Length: 2679 bp

>NTDB_id=391137 DAAJ005_RS03185 WP_151845851.1 235917..238595(-) (pilB) [Deinococcus sp. AJ005]
ATGGCACTTTCTATCGGTGACCGCCGACTGGGCGCGATTCTGCTCGAACAGGGCTACGTCAACGACGGCGATCTGCAAAA
GGCGCTGGTCCGCCACTCCGAAGTCGGCGGACGGCTGGCTGAGATTTTAATCGACACCGGGCTGGTGGGGGAAAAACGCA
TTGCGCGGGCCATCGAGGAAGCGCTGGGCATTCCGCTGGTCAACCTGCTGGCGATCACGCCGGACCCGGCAGCGGTGGCG
GCGATCCCGGCCCAGACGGCGCTGAATTTGCAGGCCATCCCGTTTGCACTGGAAAACGGCGTGCTGCGTGTGGCCCTGGT
CGACCCGCTGTCCAGTCTGGCGCTGGAAACGCTGGAGGACGACAGCAACCTGCCCATCGAGCCGTATCAGGCGCTGCGCG
AGCAGGTACTGTGGGCGGTGGCCACACACTACCCAGAACTGGGACTGACCCCCGTCGCGCCCGCCGATTTTGGCGAGGAA
GGGCCGGGCGGGCAACTGGGGCAGGCACTGATCGCACGCGGGCTGGTCACCGACGCGCAGCTTCAGGTGGCCCTGGACGC
CCAGCAGCAGACTGGCGAGGCGCTGGGCAACACGCTGGTGGCCCAGAAGGCCATTACGGAAGACCAGCTCTATGAACTGC
TGGCCGAGCAAAGCGACGTCATCTACCTGAAAAATCCGCGCGACTTCCAGCCCGCAGAGGACGTGCTGGGCAGCATGCTG
CGGGCCGACGCTCTGCGCCTGTCCGCCGTGCCCGTCGACGAGACCGATGAACATGTGGTGGTGGTCACCAGTGACCCGCG
CAAGCGTGATGACATCGCCGCGCTGATCGGGCGGCCCATCCAGCTCATCTTAGCGCGGCCCCGCGATCTGGAATTCCTGA
CCGAGCGCTTTTACCCTCAGCGTGGCCGTCTGGGCGAGCAGATGGTGCAGGAAGGCACCCTGTCGCGCCAGCAACTGCGT
GAGGCGTTGCAGGTGCAGGCCCGCGCGGGCAAGGTCAAGCCGCTGGGCGAGGTCATTCTGGACCTGGGCTTTGCCGACGC
CAGCGCGATCGACAGCGCCCTGCAAAAGCAGAACGTGGGCGGAGGACGCCTGGAAGACACCCTGGTGCAGTCGGGCAAAC
TGTCGCCGGAGATGCTGGCGCGCTCGCTGGCCGCGCAACTGGGCTACGAATTCCTTGATCCAGTCCAGAATCCCCCAGAC
GCCAAGGTGGCCCTGCTGATCCCTGAACCCACCGCACGGCGCTACGGCGTGGTGCCGGTCCGGTTGCAGGGCGAGTCGCT
GGTCATCGCCATGAAGGACCCGCGCAACGTGTTTGCGCTGGACGATCTGAAATTGATCACCGGGCGCGACATCATTCCCG
CCGTGATGGCGGAAAAGGACATCATCCGGCTGATCGAGCGCTACTTCGGCAACCAGGGCATGGCGGACCTGAACCAGCGC
CTCCAGACCGAGTCCCTGCAACGCGAGAAGGATGCCAAACGCGGCAACGACGTCGACGTGAACGCCGGATTCGACGACAA
CGCCGTGGTCAAGGTGGTGGACGGCCTAATCCGCGAGGCGGCCTTGCAGGAAGCCAGCGACATCCACATCGAACCCACCG
AATTCAGCCTGCGTATCCGCTACCGCGTGGACGGCATCCTGCGCGAGCAGAACGAGCTGCCCAAGGGGGCCGCACAGAGC
ATCCTGGCACGCATCAAGATCATGGGTAACCTCGACATTTCCGAGCGGCGGGTGCCGCAGGACGGACGCGTGCGTTTCAA
GAAGGGCAGCATCGACATCGATCTGCGTCTGTCCACCCTGCCCACTGTCTACGGCGAGAAAGCCGTGATGCGACTGCTCC
AGAAGGCCAGCAACATCCCCGAAGTCGAACAACTGGGCTTCAGCGACCACAACTTCCAGCGCTACCTGGACGTGATCGAC
AAGCCCAACGGCATCTTCCTGATCACCGGGCCGACGGGTTCGGGCAAGTCGTTTACCAGTTTTTCCACCCTCAAGCGCAT
CGCCAAGCCCGAGAAGAACACCACCACCATCGAGGACCCGGTGGAATACGAGATTCCCGGCATCATCCAGACCCAGGTCA
ACCCGGTGGCGGGCCTGACGTTTGCCCGCGCCCTGCGCGCCTTTTTGCGGCAGGACCCCGACATTATCTTCGTGGGGGAA
ATCCGGGACACCGAAACGGCCAAGATTGCCGTGGAAGCCGCCCTGACTGGTCACCTGGTGTTGGCGACGCTGCACACCAA
CGACGCACCCGGTGCCATCGTGCGCCTGGAGGAAATGGGCGTGGAGCCGTTCAATATCAGCGCCTCGGTGGTGGGCGTGC
TGGCCCAGCGCCTGGTCCGCAAGGTCTGCCCCGACTGTAAGGCCCCCACCAACGCGGACCCGGACGTGCTGCGCCGACTG
GGCATCACCGACCGCGACCTGCGCGGCGCACAACTGATGCGCGGCGCGGGCTGTGCGCGTTGCGGCGGCACCGGCTACAA
GGGCCGCATGGGCATCCACGAGCTGATGGTGGTGGACGAACCGCTGCGTGTGGCCATCGGGACAGGCAAGCCTGCCACCG
AAATCCGCGACGTGGCCCTGGAGAAGAGCGGCATGAAAACCCTGCGCCAGGACGGGATCGAGAAGGCCCTCAAGGGCATC
ACCACCCTGGAAGAAGTGCTGGCTGTGACCAGCAATTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-473)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

• Type II/IV secretion system protein (T2SSE) (517-781)

3D structure

• AlphaFold DB: A0A5J6YPY9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	77.044	100	0.771
pilF	Thermus thermophilus HB27	56.425	100	0.566