Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D6T76_RS10420 Genome accession   NZ_CP044335
Coordinates   2215237..2216217 (-) Length   326 a.a.
NCBI ID   WP_169723101.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain AKB48     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2210237..2221217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6T76_RS10390 (D6T76_10535) - 2211115..2211612 (+) 498 WP_169723098.1 SprT family zinc-dependent metalloprotease -
  D6T76_RS10395 (D6T76_10540) endA 2211707..2212414 (+) 708 Protein_2049 deoxyribonuclease I -
  D6T76_RS10400 (D6T76_10545) rsmE 2212466..2213197 (+) 732 WP_169723099.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D6T76_RS10405 (D6T76_10550) gshB 2213229..2214176 (+) 948 WP_015571835.1 glutathione synthase -
  D6T76_RS10410 (D6T76_10555) - 2214251..2214811 (+) 561 WP_169723100.1 YqgE/AlgH family protein -
  D6T76_RS10415 (D6T76_10560) ruvX 2214811..2215227 (+) 417 WP_033487645.1 Holliday junction resolvase RuvX -
  D6T76_RS10420 (D6T76_10565) pilT 2215237..2216217 (-) 981 WP_169723101.1 type IV pilus twitching motility protein PilT Machinery gene
  D6T76_RS10425 (D6T76_10570) - 2216235..2216936 (+) 702 WP_017382925.1 YggS family pyridoxal phosphate-dependent enzyme -
  D6T76_RS10430 (D6T76_10575) - 2216958..2217524 (+) 567 WP_169723102.1 YggT family protein -
  D6T76_RS10435 (D6T76_10580) yggU 2217521..2217817 (+) 297 WP_169723103.1 DUF167 family protein YggU -
  D6T76_RS10440 (D6T76_10585) - 2217821..2218414 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  D6T76_RS10445 (D6T76_10590) hemW 2218407..2219555 (+) 1149 WP_169723104.1 radical SAM family heme chaperone HemW -
  D6T76_RS10450 (D6T76_10595) - 2219612..2219950 (+) 339 WP_047726774.1 endonuclease domain-containing protein -
  D6T76_RS10455 (D6T76_10600) - 2220033..2220749 (-) 717 WP_169723105.1 DUF2884 domain-containing protein -
  D6T76_RS10460 (D6T76_10605) - 2220806..2221132 (-) 327 WP_169723106.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35604.55 Da        Isoelectric Point: 6.5005

>NTDB_id=389490 D6T76_RS10420 WP_169723101.1 2215237..2216217(-) (pilT) [Enterobacter hormaechei strain AKB48]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRPALTAAETGHLVLATLHTRGAAQAIERPVDT
FPAQEKDPVRNQLAGSLRAVPAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTRQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=389490 D6T76_RS10420 WP_169723101.1 2215237..2216217(-) (pilT) [Enterobacter hormaechei strain AKB48]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAAGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTCGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCCGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCCGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCCGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCCGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment