Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DDA3937_RS17295 Genome accession   NC_014500
Coordinates   3937527..3938540 (-) Length   337 a.a.
NCBI ID   WP_013319311.1    Uniprot ID   E0SEF4
Organism   Dickeya dadantii 3937     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3932527..3943540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDA3937_RS17275 (Dda3937_02605) - 3934272..3935183 (+) 912 WP_013319308.1 PucR family transcriptional regulator -
  DDA3937_RS17280 (Dda3937_02604) codA 3935186..3936436 (+) 1251 WP_033112043.1 cytosine deaminase -
  DDA3937_RS17285 (Dda3937_02603) - 3936591..3937211 (+) 621 WP_013319310.1 Fic/DOC family protein -
  DDA3937_RS17290 - 3937190..3937381 (-) 192 WP_033112044.1 hypothetical protein -
  DDA3937_RS17295 (Dda3937_02602) pilT 3937527..3938540 (-) 1014 WP_013319311.1 type IV pilus twitching motility protein PilT Machinery gene
  DDA3937_RS17300 (Dda3937_02601) - 3938562..3939296 (+) 735 WP_013319312.1 YggS family pyridoxal phosphate-dependent enzyme -
  DDA3937_RS17305 (Dda3937_02600) proC 3939330..3940151 (+) 822 WP_013319313.1 pyrroline-5-carboxylate reductase -
  DDA3937_RS17310 (Dda3937_02599) - 3940195..3940749 (+) 555 WP_013319314.1 YggT family protein -
  DDA3937_RS17315 (Dda3937_02598) yggU 3940746..3941036 (+) 291 WP_033112045.1 DUF167 family protein YggU -
  DDA3937_RS17320 (Dda3937_02597) - 3941083..3941676 (+) 594 WP_013319316.1 XTP/dITP diphosphatase -
  DDA3937_RS17325 (Dda3937_02596) hemW 3941669..3942808 (+) 1140 WP_013319317.1 radical SAM family heme chaperone HemW -
  DDA3937_RS17330 (Dda3937_02595) - 3942933..3943061 (+) 129 WP_013319318.1 entericidin A/B family lipoprotein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 36737.84 Da        Isoelectric Point: 6.3642

>NTDB_id=38360 DDA3937_RS17295 WP_013319311.1 3937527..3938540(-) (pilT) [Dickeya dadantii 3937]
MDVSEWVRSSVKQNVSDLHLCSGHPPIWRIDGQLVADAAQPPLHNAELEQWCHSWLEPACEQQLRQSGQTDCALTLPEGQ
RLRANLFMQRHGLSAALRLIPSHQPDLQTLGAPPVVAELAERADGLILVTGATGSGKSTTLAAMIAALNQRSARHIITLE
DPIEFLHTSQRSLIQQREIGRHSEGFASALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRQAVQAVDR
LVDVFPAEEKAFVRAQLAASLQAVVAQKLLPGAQGGRVALFEVLLATPAVSNLIREGKTHQVPSLLQTGSQVGMQTFEQS
YQQRCREGVLRPEINAD

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=38360 DDA3937_RS17295 WP_013319311.1 3937527..3938540(-) (pilT) [Dickeya dadantii 3937]
ATGGATGTGAGTGAATGGGTGCGGTCTAGTGTAAAACAGAATGTGTCCGATCTGCACCTGTGCAGCGGTCATCCGCCGAT
TTGGCGTATCGACGGGCAACTGGTGGCGGATGCCGCGCAGCCGCCGTTGCACAACGCCGAACTGGAACAGTGGTGCCACA
GTTGGCTGGAGCCGGCCTGCGAGCAGCAGCTACGGCAAAGCGGGCAGACCGATTGCGCGCTGACGCTGCCGGAGGGCCAG
CGGTTACGGGCAAACCTGTTTATGCAGCGCCACGGCCTGTCGGCGGCGTTACGTCTGATCCCGTCACACCAACCCGACCT
GCAAACGCTGGGCGCGCCGCCGGTGGTGGCGGAGCTGGCCGAGCGCGCCGATGGCCTGATTCTGGTGACCGGCGCCACCG
GCAGCGGTAAATCCACCACCCTGGCGGCGATGATTGCCGCGCTTAATCAACGCAGCGCACGCCATATCATTACGCTGGAA
GACCCGATTGAGTTCCTGCATACCAGCCAGCGCAGCCTGATCCAGCAGCGGGAGATCGGCCGTCACAGCGAAGGATTCGC
CAGCGCGCTGCGGGCGGCGCTGCGCGAAGACCCGGACGTACTGTTGCTCGGCGAGTTGCGCGACAGCGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTACTGGCGACGCTGCATACCCGGCAGGCGGTGCAGGCGGTGGATCGG
CTGGTGGACGTCTTCCCCGCCGAGGAGAAAGCGTTTGTCCGCGCGCAACTGGCCGCCAGCCTGCAGGCGGTAGTGGCGCA
GAAACTGTTGCCGGGCGCGCAAGGCGGGCGCGTGGCGCTGTTTGAAGTGCTGCTCGCCACCCCGGCGGTCAGCAACTTGA
TCCGCGAAGGCAAAACCCACCAGGTGCCCAGCCTGCTGCAAACCGGCAGCCAGGTCGGTATGCAGACTTTTGAACAGAGT
TACCAGCAGCGGTGCCGGGAGGGGGTGCTGCGGCCTGAGATCAATGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0SEF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.254

99.407

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

97.923

0.49
  pilT Vibrio cholerae strain A1552

50

97.923

0.49
  pilT Neisseria gonorrhoeae MS11

49.102

99.11

0.487
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.923

0.475
  pilT Pseudomonas aeruginosa PAK

47.748

98.813

0.472
  pilT Acinetobacter baumannii strain A118

47.447

98.813

0.469
  pilT Acinetobacter baumannii D1279779

47.447

98.813

0.469
  pilT Acinetobacter nosocomialis M2

47.447

98.813

0.469
  pilT Acinetobacter baylyi ADP1

47.576

97.923

0.466
  pilT Legionella pneumophila strain ERS1305867

47.273

97.923

0.463
  pilT Legionella pneumophila strain Lp02

47.273

97.923

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.455

94.659

0.43
  pilU Pseudomonas stutzeri DSM 10701

37.725

99.11

0.374
  pilU Vibrio cholerae strain A1552

38.889

96.142

0.374

Multiple sequence alignment