Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HF908_RS04115 Genome accession   NZ_CP051171
Coordinates   859455..860594 (+) Length   379 a.a.
NCBI ID   WP_193027656.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2279     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 854455..865594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF908_RS04080 (HF908_04140) - 854505..854747 (+) 243 WP_011002597.1 hypothetical protein -
  HF908_RS04085 (HF908_04145) - 854906..855406 (+) 501 WP_003271865.1 Dps family protein -
  HF908_RS04090 (HF908_04150) ubiA 855480..856355 (+) 876 WP_193027651.1 4-hydroxybenzoate octaprenyltransferase -
  HF908_RS04095 (HF908_04155) - 856352..856630 (-) 279 WP_193027652.1 hypothetical protein -
  HF908_RS04100 (HF908_04160) proC 856653..857477 (-) 825 WP_193027653.1 pyrroline-5-carboxylate reductase -
  HF908_RS04105 (HF908_04165) - 857512..858234 (-) 723 WP_193027654.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF908_RS04110 (HF908_04170) pilT 858359..859402 (+) 1044 WP_193027655.1 type IV pilus twitching motility protein PilT Machinery gene
  HF908_RS04115 (HF908_04175) pilU 859455..860594 (+) 1140 WP_193027656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF908_RS04120 (HF908_04180) pilE 860789..861229 (+) 441 WP_193027657.1 type IV pilin protein Machinery gene
  HF908_RS04125 (HF908_04185) - 861233..861721 (+) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  HF908_RS04130 (HF908_04190) pilV 861718..862308 (+) 591 WP_193027658.1 type IV pilus modification protein PilV -
  HF908_RS04135 (HF908_04195) - 862305..863333 (+) 1029 WP_193027659.1 PilW family protein -
  HF908_RS04140 (HF908_04200) - 863337..863873 (+) 537 WP_193027660.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42235.85 Da        Isoelectric Point: 6.9273

>NTDB_id=383002 HF908_RS04115 WP_193027656.1 859455..860594(+) (pilU) [Ralstonia pseudosolanacearum strain RUN2279]
MLDRDAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTIVQAMGLVKAIMSEKQLREFEESFECNFA
ITAPATGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEIHELKEVMKKSREQ
GMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=383002 HF908_RS04115 WP_193027656.1 859455..860594(+) (pilU) [Ralstonia pseudosolanacearum strain RUN2279]
ATGCTGGACCGCGACGCCGCCACCAAATATATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACCGCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGCTGACCATCGTCC
AGGCCATGGGCCTGGTCAAGGCGATCATGAGCGAGAAGCAGCTGCGCGAGTTCGAAGAGTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCGCCACCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CACCACCAAGATCCCGACGCTCGCCGAACTGGACCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTGGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATATGTGCACGCACACCACAACTGCGTTGTGACGCAGCGCGAGGTGGG
CGTGGACACGGAATCCTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAGTACGCCATCCAGTACGCTGAGACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGTCAGCAACTGCTGATCGACCTGTCCCTGAA
CCTGCGCGCCATGATCGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGATACACGAGCTCAAGGAGGTCATGAAGAAGTCGCGCGAGCAG
GGCATGGTCTGTTTCGACCAAGCGCTGTTCGAGCTCTACGAGGCGGACAAGATCACCTATGAGGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTTTGCAGATCAAGCTGCACAGCAAGCGCGGCGGTCCGGTGGACCTGACGGCCGGCACCG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(87-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.04

93.14

0.578
  pilU Acinetobacter baylyi ADP1

55.71

94.723

0.528
  pilU Vibrio cholerae strain A1552

55.367

93.404

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Legionella pneumophila strain Lp02

43.844

87.863

0.385
  pilT Legionella pneumophila strain ERS1305867

43.844

87.863

0.385
  pilT Pseudomonas aeruginosa PAK

42.433

88.918

0.377
  pilT Vibrio cholerae strain A1552

45.11

83.641

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.11

83.641

0.377
  pilT Acinetobacter baylyi ADP1

44.099

84.96

0.375
  pilT Pseudomonas stutzeri DSM 10701

41.246

88.918

0.367
  pilT Acinetobacter baumannii D1279779

43.26

84.169

0.364
  pilT Acinetobacter baumannii strain A118

43.26

84.169

0.364
  pilT Acinetobacter nosocomialis M2

43.26

84.169

0.364

Multiple sequence alignment