Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HF908_RS04120 Genome accession   NZ_CP051171
Coordinates   860789..861229 (+) Length   146 a.a.
NCBI ID   WP_193027657.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2279     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 855789..866229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF908_RS04095 (HF908_04155) - 856352..856630 (-) 279 WP_193027652.1 hypothetical protein -
  HF908_RS04100 (HF908_04160) proC 856653..857477 (-) 825 WP_193027653.1 pyrroline-5-carboxylate reductase -
  HF908_RS04105 (HF908_04165) - 857512..858234 (-) 723 WP_193027654.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF908_RS04110 (HF908_04170) pilT 858359..859402 (+) 1044 WP_193027655.1 type IV pilus twitching motility protein PilT Machinery gene
  HF908_RS04115 (HF908_04175) pilU 859455..860594 (+) 1140 WP_193027656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF908_RS04120 (HF908_04180) pilE 860789..861229 (+) 441 WP_193027657.1 type IV pilin protein Machinery gene
  HF908_RS04125 (HF908_04185) - 861233..861721 (+) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  HF908_RS04130 (HF908_04190) pilV 861718..862308 (+) 591 WP_193027658.1 type IV pilus modification protein PilV -
  HF908_RS04135 (HF908_04195) - 862305..863333 (+) 1029 WP_193027659.1 PilW family protein -
  HF908_RS04140 (HF908_04200) - 863337..863873 (+) 537 WP_193027660.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15203.22 Da        Isoelectric Point: 8.6699

>NTDB_id=383003 HF908_RS04120 WP_193027657.1 860789..861229(+) (pilE) [Ralstonia pseudosolanacearum strain RUN2279]
MASKLACQRARSARGFTLIELMIVAAIVAILAVLAYPSYVQYIVRSNRAATESFMQEVAAAQERFLLDNRAYASDLATLQ
YASSVPASVAANYQFSFTAVTTAPPAYTLNATPAGSQASSDAACGTLGLSNTGSKTASGGASNCWK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=383003 HF908_RS04120 WP_193027657.1 860789..861229(+) (pilE) [Ralstonia pseudosolanacearum strain RUN2279]
GTGGCAAGCAAGCTTGCATGCCAGCGCGCGCGGTCAGCGCGGGGCTTCACGCTGATCGAGCTCATGATCGTGGCGGCTAT
TGTCGCCATTCTTGCGGTTCTGGCGTATCCCTCTTACGTTCAGTACATCGTGCGCTCCAACCGCGCGGCGACGGAATCGT
TCATGCAGGAAGTGGCTGCGGCCCAGGAGCGCTTCCTGCTCGATAACCGAGCGTATGCCTCGGACCTGGCGACGCTGCAA
TATGCCAGCAGCGTTCCGGCCAGCGTCGCCGCCAACTACCAGTTCAGCTTCACTGCCGTTACGACGGCTCCGCCGGCGTA
TACGCTGAATGCAACGCCGGCGGGCAGCCAGGCCTCCAGCGACGCCGCGTGCGGCACGCTCGGCCTGTCGAACACCGGCA
GCAAGACGGCCTCCGGCGGTGCCTCCAACTGCTGGAAATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-36)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(37-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

40.299

91.781

0.37
  comF Acinetobacter baylyi ADP1

40

92.466

0.37
  pilA Ralstonia pseudosolanacearum GMI1000

40.769

89.041

0.363

Multiple sequence alignment